Lake-Associated Outbreak of Escherichia coli O157:H7 in Clark County, Washington, August 1999

Bruce, Michael G.; Curtis, Michael G.; Payne, Melanie M.; Gautom, Romesh; Thompson, Eric C.; Bennett, Aimee L.; Kobayashi, John M.

doi:10.1001/archpedi.157.10.1016

Cited by 45 publications

(32 citation statements)

References 23 publications

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“…Escherichia coli O157:H7 is one of the most dangerous agents of food-borne diseases (1). Several of the reported outbreaks of E. coli O157:H7 have led to death, especially in cases involving children and the elderly (2)(3)(4)(5). Given the low infectious dose of E. coli O157:H7 (Ϸ10-100 cells), the presence of even a single bacterium in food may pose a serious health risk (1,6).…”

mentioning

confidence: 99%

A rapid bioassay for single bacterial cell quantitation using bioconjugated nanoparticles

Zhao

Hilliard

Mechery

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

530

356

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The rapid and sensitive determination of pathogenic bacteria is extremely important in biotechnology, medical diagnosis, and the current fight against bioterrorism. Current methods either lack ultrasensitivity or take a long time for analysis. Here, we report a bioconjugated nanoparticle-based bioassay for in situ pathogen quantification down to single bacterium within 20 min. The bioconjugated nanoparticle provides an extremely high fluorescent signal for bioanalysis and can be easily incorporated with biorecognition molecules, such as antibody. The antibody-conjugated nanoparticles can readily and specifically identify a variety of bacterium, such as Escherichia coli O157:H7, through antibody-antigen interaction and recognition. The single-bacterium-detection capability within 20 min has been confirmed by the plate-counting method and realized by using two independent optical techniques. The two detection methods correlated extremely well. Furthermore, we were able to detect multiple bacterial samples with high throughput by using a 384-well microplate format. To show the usefulness of this assay, we have accurately detected 1-400 E. coli O157 bacterial cells in spiked ground beef samples. Our results demonstrate the potential for a broad application of bioconjugated nanoparticles in practical biotechnological and medical applications in various biodetection systems. The ultimate power of integrating bionanotechnology into complex biological systems will emerge as a revolutionary tool for ultrasensitive detection of disease markers and infectious agents.

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mentioning

confidence: 99%

A rapid bioassay for single bacterial cell quantitation using bioconjugated nanoparticles

Zhao

Hilliard

Mechery

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

530

356

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“…Waterborne disease outbreaks caused by E. coli O157:H7 are primarily associated with recreational lake waters (Bruce et al 2003). This is due to the ingestion of untreated water during swimming.…”

Section: Surface and Ground Watersmentioning

confidence: 99%

“…Domestic and wild animals are believed to be the source of contamination in most recreational surface waters. For example, following a 1999 recreational outbreak in Battle Ground Lake, Washington, E. coli O157:H7 was isolated from duck feces as well as the water (Samadpour et al 2002;Bruce et al 2003). Kurokawa et al (1999) detected 10 2 to 10 5 E. coli O157:H7 per mL in river water heavily polluted by industrial and agricultural wastes using fluorescence antibodies.…”

Section: Surface and Ground Watersmentioning

confidence: 99%

Occurrence and persistence of Escherichia coli O157:H7 in water

Nwachuku

Gerba

2008

Rev Environ Sci Biotechnol

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“…GI illnesses are the most frequent diseases associated with activities in recreational waters but they are usually mild and self-limiting, which can lead to some difficulties in their monitoring. Nonetheless, epidemiological studies have shown positive associations between sporadic cases of GI illness and activities in recreational waters and between fecal contamination of these waters and GI outbreaks [4][5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Monitoring Recreational Waters: How to Integrate Environmental Determinants

Turgeon¹

2012

JEP

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Recreational waters are associated with a higher risk of disease for people engaged in activities that bring them into contact with these waters. The primary cause of contamination of recreational waters is fecal microorganisms, which may originate from various sources and involve several modulating factors, making it a complex public health and environmental issue. Monitoring recreational water quality should include two key components: Microbial water testing and monitoring environmental determinants associated with higher risks of contamination. Conducting both activities provides the foundation for a comprehensive assessment according to risk and the actual level of fecal pollution and thus could promote good management actions to ensure safe water quality. Nevertheless, monitoring of environmental determinants is rarely fully integrated in monitoring programs and is also harder to achieve, especially when water pollution is mainly associated with nonpoint sources. In order to achieve identification and monitoring of environmental determinants associated with fecal contamination of recreational waters, some specific steps should be followed and some questions must be answered. The objective of this review article is to present current knowledge on this topic and to suggest and discuss recommendations. Potential sources of contamination and factors able to modulate them should be identified and measured after the geographical area influencing fecal contamination of recreational water has been delineated. Statistical models have been developed to identify the relative importance of these environmental characteristics on fecal pollution of recreational waters but they do not allow for a full comprehension of the exact processes leading to this pollution, thus other methods should also be used to better understand these processes.

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Lake-Associated Outbreak of Escherichia coli O157:H7 in Clark County, Washington, August 1999

Cited by 45 publications

References 23 publications

A rapid bioassay for single bacterial cell quantitation using bioconjugated nanoparticles

A rapid bioassay for single bacterial cell quantitation using bioconjugated nanoparticles

Occurrence and persistence of Escherichia coli O157:H7 in water

Monitoring Recreational Waters: How to Integrate Environmental Determinants

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