Laju Nitrifikasi pada Bioremediasi Air Limbah Organik Menggunakan Chlorella sp. dan Bakteri Nitrifikasi-Denitrifikasi

Hendrawan, Arya Ksatria Fernanda; Afiati, Norma; Rahman, Arif

doi:10.29244/jpsl.11.2.309-323

Cited by 4 publications

(4 citation statements)

References 28 publications

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“…According to (Ngibad 2019) stated that the started concentration of ammonium in the water Ngelom's river is 1.61-14.8 mg/L. According to the study (Hartono et al 2016), the ammonium concentration produced by bacteria in the soil is 256.7 μM up to 1027.77 μM (0.2567-1.0277 mg/L), while according to a study (Hendrawan et al 2021), in the freshwater of Rawa Pening Lake, it shows that the ammonium produced by bacteria through the nitrification process reaches 0.2 mg/L/hour. Ammonium can be synthesized by the nitrogen-fixing bacteria in the soil or the water.…”

Section: Ammonium Semi-quantitative and Quantitative Assaymentioning

confidence: 99%

Exploration non-symbiotic nitrogen-fixing bacteria from several lakes in East Java, Indonesia

et al. 2022

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Abstract. Nafisah W, Prabaningtyas S, Witjoro A, Saptawati RT, Rodiansyah A. 2022. Exploration non-symbiotic nitrogen-fixing bacteria from several lakes in East Java, Indonesia. Biodiversitas 23: 1752-1758. Potential microorganisms, mainly nitrogen-fixing bacteria spread in almost lake areas in East Java. The biogeochemical processes that occur between biotic-biotic or biotic-abiotic factors can accelerate the growth of autotrophic microorganisms in waters as like microalgae. Non-symbiotic nitrogen-fixing bacteria in the water can supply nitrogen to support the growth of microalgae. This study is aimed to explore and calculate the ammonium concentration produced by non-symbiotic nitrogen-fixing bacteria that isolated from several lakes in East Java. The highest ammonium producing bacteria were characterized based on morphology, physiology, and genetic with 16S rRNA gene sequencing. The bacterial isolates were screened with a nitrogen-free bromothymol blue medium to select bacterial isolates capable of fixing nitrogen. The content of NH3/NH4 was produced by isolates of nitrogen-fixing bacteria was measured by using the prodac test kit NH3/NH4. The ammonium concentration was calculated with Nessler’s reagent by using the spectrophotometry method at a wavelength of 425 nm. The result was obtained 31 isolates of non-symbiotic nitrogen-fixing bacteria. The bacterial isolate B2 exhibited highest activity of fixing nitrogen that produced ammonium (14.46 mg/L) among the other screened bacteria. The morphological and physiological characterization from that isolate was identified as genus Bacillus, whereas the genetic characterization similar to Bacillus paramycoides, that included in the Bacillus cereus group.

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Section: Ammonium Semi-quantitative and Quantitative Assaymentioning

confidence: 99%

Exploration non-symbiotic nitrogen-fixing bacteria from several lakes in East Java, Indonesia

et al. 2022

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“…Díez-Montero et al (2020) observed that a high pH, owing to photosynthetic suppression, resulted in the buildup of inorganic and organic contents in Chlorella media. Hendrawan et al (2021) also explained that ammonium (NH 4 + ) buildup might impede photosynthesis. Chlorella sp.…”

Section: Ph Valuementioning

confidence: 99%

Impact of disposable mask microplastics pollution on the aquatic environment and microalgae growth

Khoironi

Hadiyanto

Hartini

et al. 2023

Environ Sci Pollut Res

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The COVID-19 pandemic has mandated people to use medical masks to protect the public. However the improper management of disposable mask waste has led to the increase of marine pollution, in terms of water quality, and the decline in aquatic microorganisms. The aim of this research was to investigate the impact of disposable mask waste on fresh water and microalgae biomass quality. Disposable masks (untreated or treated with Enterococcus faecalis ) were placed in 10-L glass reactors containing fresh water or water containing algal Chlorella sp. and its growth supplements ( Chlorella medium) (four 10-L reactors in total) and kept in controlled conditions for 3 months. Water and biomass yield quality were evaluated using water quality analysis, spectroscopy, scanning electron microscopy (SEM), and proximate lipid and protein analysis. Disposable masks, incubated in either fresh water or Chlorella medium, affected several water quality parameters such as chemical oxygen demand (COD), biological oxygen demand (BOD), dissolved oxygen (DO), and pH. Microplastic identification revealed that some fibers were present in the water following a 100-day treatment process. Fourier transform–infrared spectroscopy (FTIR) analysis was used to determine the change in important, organic functional groups and highlighted the disappearance of a peak at 1530 cm −1 corresponding to the primary protein (C–N) and the appearance of new peaks at 1651 cm −1 and 1270 cm −1 corresponding to methyl alcohol (CH 2 OH) and ketone (C = O), respectively. This indicated the detrimental effect of disposable mask fragmentation on the biomass quality. The SEM investigation has shown a damage to the surface membrane of Chlorella sp. cells. Altogether, disposable masks decreased the water quality and damaged microalgae by inhibiting their growth. Therefore, the disposable mask contaminated by various microbes, after being used by a human, may be one of the most dangerous hazards to the environment.

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“…Gambar.2: Diagram rata-rata total kadar nitrogen (N) pada perlakuan penambahan MOL air beras Mikroorganisme lokal pada air cucian beras yang ditambahkan dari air cucian beras dapat berperan dalam siklus nitrogen pada pupuk organik. Mikroorganisme seperti bakteri nitrifikasi dapat membantu dalam konversi amonium (NH4+) menjadi nitrat (NO3-), yang merupakan bentuk nitrogen yang lebih mudah digunakan oleh tanaman (Pradipta, 2016).Selain itu, mikroorganisme lain seperti bakteri denitrifikasi dapat mengkatalisasi konversi nitrat menjadi nitrogen gas (N2), menghasilkan efek yang mengurangi kadar nitrogen dalam pupuk organik (Hendrawan et al, 2021). Proses denitrifikasi terjadi dalam kondisi anaerobik (tanpa oksigen) dan dapat terjadi dalam lingkungan tertentu, seperti lingkungan tanah yang lembab.…”

Section: Nitrogen (N)unclassified

Pengaruh Level Konsentrasi Penambahan Mikroorganisme Lokal Air Cucian Beras Terhadap Kualitas Pupuk Organik

Triasih,

Erni

2023

agribios

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Penelitian ini bertujuan untuk meneliti tentang pengaruh penambahan mikroorgasnisme lokal air cucian beras terhadap kadar karbon pupuk kotoran sapi. Penelitian ini dianalisis dengan ANOVA dan dilanjutkan dengan uji beda rataan menurut Duncan (DMRT). Diperoleh hasil penelitian bahwa penambahan mikroorgasnisme lokal air cucian beras berpengaruh nyata terhadap kadar kualitas karbon (C), Nitrogen (N), Fosfor (P), Kalium (K) dan pH pupuk organik kotoran sapi. Sementara itu, faktor warna dan bau pada pupuk organik kotoran sapi tidak mempengaruhi penambahan mikroorgasnisme lokal air cucian beras. Di sisi lain, pemberian mikroorgasnisme lokal (MOL) pada air cucian beras dengan kadar 20% memberikan kadar kualitas fisik kimia pada pupuk organik yang optimal apabila dibandingkan dengan pemberian MOL dengan kadar 0%, 5%, 10%, dan 15%.

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Laju Nitrifikasi pada Bioremediasi Air Limbah Organik Menggunakan Chlorella sp. dan Bakteri Nitrifikasi-Denitrifikasi

Cited by 4 publications

References 28 publications

Exploration non-symbiotic nitrogen-fixing bacteria from several lakes in East Java, Indonesia

Exploration non-symbiotic nitrogen-fixing bacteria from several lakes in East Java, Indonesia

Impact of disposable mask microplastics pollution on the aquatic environment and microalgae growth

Pengaruh Level Konsentrasi Penambahan Mikroorganisme Lokal Air Cucian Beras Terhadap Kualitas Pupuk Organik

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