lacZ Transgenic mice to monitor gene expression in embryo and adult

Schmidt, Andrea; Tief, Kirsten; Foletti, Alessandro; Hunziker, Agnès; Penna, Doris; Hummler, Edith; Beermann, Friedrich

doi:10.1016/s1385-299x(98)00021-x

Cited by 32 publications

(28 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Genomic DNA from embryonic stem cell clones and from mouse tails was prepared as described (20). Southern blot analysis was performed on DNA digested with HincII according to standard procedures by using probe A (Fig.…”

Section: Methodsmentioning

confidence: 99%

Salt restriction induces pseudohypoaldosteronism type 1 in mice expressing low levels of the β-subunit of the amiloride-sensitive epithelial sodium channel

Pradervand

Barker

Wang

et al. 1999

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

128

109

View full text Add to dashboard Cite

The amiloride-sensitive epithelial sodium channel (ENaC) is a heteromultimer of three homologous subunits (␣-, ␤-, and ␥-subunits). To study the role of the ␤-subunit in vivo, we analyzed mice in which the ␤ENaC gene locus was disrupted. These mice showed low levels of ␤ENaC mRNA expression in kidney (Ϸ1%), lung (Ϸ1%), and colon (Ϸ4%). In homozygous mutant ␤ENaC mice, no ␤ENaC protein could be detected with immunof luorescent staining. At birth, there was a small delay in lung-liquid clearance that paralleled diminished amiloride-sensitive Na ؉ absorption in tracheal explants. With normal salt intake, these mice showed a normal growth rate. However, in vivo, adult ␤ENaC m͞m mice exhibited a significantly reduced ENaC activity in colon and elevated plasma aldosterone levels, suggesting hypovolemia and pseudohypoaldosteronism type 1. This phenotype was clinically silent, as ␤ENaC m͞m mice showed no weight loss, normal plasma Na ؉ and K ؉ concentrations, normal blood pressure, and a compensated metabolic acidosis. On low-salt diets, ␤ENaC-mutant mice developed clinical symptoms of an acute pseudohypoaldosteronism type 1 (weight loss, hyperkalemia, and decreased blood pressure), indicating that ␤ENaC is required for Na ؉ conservation during salt deprivation.

show abstract

Section: Methodsmentioning

confidence: 99%

Salt restriction induces pseudohypoaldosteronism type 1 in mice expressing low levels of the β-subunit of the amiloride-sensitive epithelial sodium channel

Pradervand

Barker

Wang

et al. 1999

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

128

109

View full text Add to dashboard Cite

show abstract

“…Fast Red substrate (Sigma, St. Louis, MO) was used as the final chromogen and hematoxylin was used as the nuclear counterstain. lacZ-stained cryostat sections (21) were counterstained with nuclear red.…”

Section: Introductionmentioning

confidence: 99%

Metastasizing Melanoma Formation Caused by Expression of Activated N-RasQ61K on an INK4a-Deficient Background

Ackermann¹,

Frutschi²,

Kaloulis³

et al. 2005

Cancer Research

Self Cite

269

303

View full text Add to dashboard Cite

show abstract

“…Tyrosinase genes have been described in numerous vertebrates (Inagaki et al 1994;reviewed in Ferguson and Kidson 1997) including the zebrafish (GenBank Accession No: AJ250302). Interestingly, while pigment rescue studies have been conducted using tyrosinase transgenes (Beermann et al 1990(Beermann et al , 1992Hyodo-Taguchi et al 1997) and tyrosinase gene promoter activity has been assayed using various marker genes (Kluppel et al 1991;Tief et al 1996Tief et al , 1997Schmidt et al 1998), the spatiotemporal pattern of endogenous tyrosinase gene transcription has not been analysed in whole embryos in a vertebrate. Here we describe the spatiotemporal pattern of the zebrafish tyrosinase gene by whole-mount in situ transcript hybridisation and we report the detection of active tyrosinase enzyme approximately 3 h before the onset of pigmentation.…”

mentioning

confidence: 99%

Tyrosinase gene expression in zebrafish embryos

Camp¹,

Lardelli²

2001

Development Genes and Evolution

View full text Add to dashboard Cite

The enzyme tyrosinase is required for the conversion of tyrosine into the pigment melanin. Thus, tyrosinase gene expression is a useful marker for studying the differentiation of melanin-expressing cells during embryogenesis. We describe the spatiotemporal pattern of transcription of the tyrosinase gene and the presence of active enzyme in whole embryos of the zebrafish, Danio rerio. At 16.5 h post-fertilisation the tyrosinase gene is transcribed in the dorsal extremity of the developing retinal pigment epithelium, approximately 7 h before visible pigmentation. Shortly thereafter, transcription in neural crest-derived melanocytes is first observed dorsolateral to the mesencephalon and diencephalon and the posterior hindbrain/anterior spinal cord. A wave of gene activation and cell migration is then observed moving towards the posterior of the animal. DOPA staining for tyrosinase activity shows the presence of active enzyme in embryos at least 3 h before visible pigmentation.

show abstract

lacZ Transgenic mice to monitor gene expression in embryo and adult

Cited by 32 publications

References 32 publications

Salt restriction induces pseudohypoaldosteronism type 1 in mice expressing low levels of the β-subunit of the amiloride-sensitive epithelial sodium channel

Salt restriction induces pseudohypoaldosteronism type 1 in mice expressing low levels of the β-subunit of the amiloride-sensitive epithelial sodium channel

Metastasizing Melanoma Formation Caused by Expression of Activated N-RasQ61K on an INK4a-Deficient Background

Tyrosinase gene expression in zebrafish embryos

Contact Info

Product

Resources

About