Lactucin &amp; Lactucopicrin ameliorates FFA-induced steatosis in HepG2 cells via modulating lipid metabolism

Aibaidula, Yilizere; Aimaiti, Mutalifu; Tan, Huiwen; Chen, Bingting; Yang, Jingfa; Ma, Xiaoli

doi:10.1016/j.jphs.2022.07.007

Cited by 10 publications

(6 citation statements)

References 42 publications

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“…Compounds 1 — 5 and 18 — 24 were evaluated for their anti‐lipid accumulation effects in an FFAs (OA/PA‐BSA, 2 : 1 ratio, 1 mmol/L)‐treated HepG2 cell model, [ 37‐39 ] with rosiglitazone used as a positive control. The MTT assay demonstrated that compounds 1 — 5 and 18 — 24 showed no toxicity against HepG2 cells at the concentration of 40 μmol/L, and the relative cell viability of them exceeded 85% (Figure S3).…”

Section: Resultsmentioning

confidence: 99%

“…The HepG2 cell line viability was assayed using MTT. [ 37‐39 ] The cells were seeded in 96‐well plates at a density of 8 × 10 3 cells/well and incubated overnight at 37 °C. The cells were then treated with medium containing various concentrations of compounds 1 , 2 , and 18 (10, 20, 40 μmol/L) and RI (10 μmol/L) along with free fatty acids (FFAs) [oleic acid/palmitic acid‐bovine serum albumin (OA/PA‐BSA) (2 : 1 ratio, 1 mmol/L)] for 24 h. After incubation, MTT solution (0.5 mg·mL −1 , 100 μL) was added to each well and the plate was incubated for 4 h at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

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Chlorajaponins A—Q, Lindenane‐Related Sesquiterpenoid Dimers from Chloranthus japonicus and Their Biological Activities

Feng,

Gu,

Huang

et al. 2024

Chin. J. Chem.

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Comprehensive SummarySeventeen undescribed lindenane‐related sesquiterpenoid dimers, chlorajaponins A—Q (1—17), and 13 reported analogs (18—30) were isolated from Chloranthus japonicus Sieb. Compound 1 possesses an unprecedented 3/5/7/5/5/6/5/3 fused octacyclic scaffold, featuring a 6(5→4)‐abeo‐lindenane monomer, while 2 exhibits a 3/5/6/6/5/6/5/3 fused octacyclic scaffold. Their structures were determined through a combination of spectroscopic analyses and X‐ray crystallography. Compounds 1, 2, and 18 demonstrated significant inhibitory effects on lipid accumulation and effectively reduced the levels of triglycerides and total cholesterol, as well as the levels of aspartate aminotransferase and alanine aminotransferase in a HepG2 cell model. In addition, compounds 1, 2, and 18 significantly suppressed the protein expression of the fatty acid synthase (FASN) and the sterol regulatory element‐binding protein 1 (SREBP1). Moreover, the anti‐inflammatory assay showed that compounds 19—22 and 25 inhibited the NO production induced by lipopolysaccharide in RAW 264.7 macrophages with IC50 values of 7.89 ± 0.44, 6.25 ± 0.46, 2.98 ± 0.29, 10.77 ± 0.60, and 3.60 ± 0.28 μmol/L.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Chlorajaponins A—Q, Lindenane‐Related Sesquiterpenoid Dimers from Chloranthus japonicus and Their Biological Activities

Feng,

Gu,

Huang

et al. 2024

Chin. J. Chem.

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“…et Huet on FFA-induced HepG2 cells. 50 Lactucin and lactucopicrin could significantly reduce the level of TG accumulation in FFA-induced HepG2 cells and could improve their lipid degeneration. After further experiments, we learned that the mechanism may be through its effect on many key genes of lipid metabolism, such as HADHA, ADAM17, SQSTM1, and GBA, which affect the regulation of lipid metabolism.…”

Section: Regulation Of Lipid Metabolismmentioning

confidence: 94%

“…These include lactucin, lactucopicrin, and 11β,13-dihydrolactucopicrin, which is the main ingredient that contributes to bitterness, and it is also a characteristic related to the promotion of human and animal appetite and digestion. 34,50,51 The compound identified by spectroscopy as 2α,6β,7β,15-tetrahydroxy-1(10),4(5)-diene-guaian-9α,12-olide-7-O-β-caffoyl-15-O-β-D-glucoside was a new sesquiterpene glycoside isolated from the methanol extract of Compositae seeds. Through a study on the toxicity of carbon tetrachloride in Wistar rats, the serum glutamic acid transaminase (SGOT) and other liver enzymes were reduced.…”

Section: Terpenoidsmentioning

confidence: 99%

“…et Huet significantly increased SOD activity, reduced GSH content, and reduced malondialdehyde (MDA) content in FFA-induced HepG2 cells, thus acting as an antioxidant. 51 A study was conducted by feeding 30-week-old mice with an AIN-93G diet or supplementing them with 0.5% freeze-dried Brussels chicory for 20 weeks. The results showed that the administration of chicory could improve oxidative stress and promote the protection of chicory against atherosclerosis.…”

Section: Antioxidantmentioning

confidence: 99%

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Research Progress on the Homologous Effects of Cichorium glandulosum Boiss. et Huet on Medicine and Food: A Review

Yang,

Lei,

Yan

et al. 2024

Natural Product Communications

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Cichorium glandulosum Boiss. et Huet is a genus of chicory in the family of Asteraceae, and it is a “medicine food homology” herbal medicine, which has the effect of detoxifying heat, diuretic and swelling, and strengthening the stomach. This paper reviews the chemical composition, pharmacological effects, and practical applications of Cichorium glandulosum Boiss. et Huet and provides a comprehensive account of its role in human health and diet, pharmacological activities, and toxicity. Cichorium glandulosum Boiss. et Huet, “medicine food homology” herbal medicine, is often widely used in compound formulations with functions such as clearing the liver, promoting gallbladder, and enhancing immunity. Previous studies have found that multiple chemical components have been isolated and identified from Cichorium glandulosum Boiss. et Huet, mainly including 89 compounds such as flavonoids, terpenoids, phenolic acids, polysaccharides, and phenylpropanoids. Meanwhile, the extract has various biological activities, including hepatoprotective and hypoglycemic, regulating blood lipids, aiding digestion, anticancer, regulating intestinal microbiota and immune function, antioxidant, and has been proven in both in vivo and in vitro studies. As an important plant resource, the current research focuses on the traditional efficacy of Cichorium glandulosum Boiss. et Huet. The chemical composition of this genus of plants is complex and their pharmacological activities are diverse. Further in-depth studies on the molecular mechanisms and toxic side effects of its specific compounds in the organism are needed. Therefore, this review provides a reference for the development and utilization of Cichorium glandulosum Boiss. et Huet.

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Lactucopicrin promotes fatty acid β‐oxidation and attenuates lipid accumulation through adenosine monophosphate‐activated protein kinase activation in free fatty acid‐induced human hepatoblastoma cancer cells

Tan,

Mi,

Tong

et al. 2024

Food Science & Nutrition

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With its annually increasing prevalence, non‐alcoholic fatty liver disease (NAFLD) has become a serious threat to people's life and health. After a preliminary research, we found that Lactucopicrin has pharmacological effects, such as lowering blood lipids and protecting the liver. Further research showed its significant activation for fatty acid β‐oxidase hydroxyacyl‐coenzyme A (CoA) dehydrogenase trifunctional multienzyme complex subunit alpha (HADHA), so we hypothesized that Lactucopicrin could ameliorate lipid accumulation in hepatocytes by promoting fatty acid β‐oxidation. In this study, free fatty acid (FFA)‐induced human hepatoblastoma cancer cells (HepG2) were used to establish an in vitro NAFLD model to investigate the molecular basis of Lactucopicrin in regulating lipid metabolism. Staining with Oil red O and measurements of triglyceride (TG) content, fatty acid β‐oxidase (FaβO) activity, reactive oxygen species (ROS) content, mitochondrial membrane potential, and adenosine triphosphate (ATP) content were used to assess the extent to which Lactucopicrin ameliorates lipid accumulation and promotes fatty acid β‐oxidation. Quantitative real‐time polymerase chain reaction (qRT‐PCR) and Western blot methods were used to explore the regulatory effects of Lactucopicrin on factors related to fatty acid β‐oxidation. Results showed that Lactucopicrin downregulated phosphorylated mammalian target of rapamycin (P‐mTOR) by activating the adenosine monophosphate‐activated protein kinase (AMPK) pathway and upregulated the messenger RNA (mRNA) and protein expression levels of coactivators (peroxisome proliferator‐activated receptor gamma coactivator 1‐alpha (PGC1α)), transcription factors (peroxisome proliferator‐activated receptor α (PPARα) and peroxisome proliferator‐activated receptor γ (PPARγ)), and oxidative factors (carnitine palmitoyltransferase 1A (CPT1A) and HADHA). This phenomenon resulted in a significant increase in FaβO activity, ATP content, and JC‐1 and a significant decrease in ROS level, TG content, and intracellular lipid droplets. With the addition of Dorsomorphin, all the effects of Lactucopicrin intervention were suppressed. In summary, Lactucopicrin promotes fatty acid β‐oxidation by activating the AMPK pathway, thereby ameliorating FFA‐induced intracellular lipid accumulation in HepG2 cells.

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Lactucin & Lactucopicrin ameliorates FFA-induced steatosis in HepG2 cells via modulating lipid metabolism

Cited by 10 publications

References 42 publications

Chlorajaponins A—Q, Lindenane‐Related Sesquiterpenoid Dimers from Chloranthus japonicus and Their Biological Activities

Chlorajaponins A—Q, Lindenane‐Related Sesquiterpenoid Dimers from Chloranthus japonicus and Their Biological Activities

Research Progress on the Homologous Effects of Cichorium glandulosum Boiss. et Huet on Medicine and Food: A Review

Lactucopicrin promotes fatty acid β‐oxidation and attenuates lipid accumulation through adenosine monophosphate‐activated protein kinase activation in free fatty acid‐induced human hepatoblastoma cancer cells

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