Lactose hydrolysis by immobilized β-galactosidase: the effect of the supports and the kinetics

Use of immobilised biocatalysts in the processing of cheese whey

“…Microorganism marxianus and applied in lactose hydrolysis processes. In spite of the fact that immobilisates showed good stability, the immobilisation yields were less than 5% [20]. ␤-Galactosidase from K. fragilis was covalently linked to silanised porous glass beads via amino groups, using glutaraldehyde.…”

Perspectives and applications of immobilised β-galactosidase in food industry - a review

“…β-Galactosidase is one of the most studied enzymes in term of its immobilisation. Although many studies described the effective immobilisation of β-galactosidase isolated from recombinant escherichia coli, its application in food industry is complicated, because this microorganism is not generally recognised as safe (GRAS) (Ladero et al 2001;Di Serio et al 2003). Currently, GRAS status is valid for A. niger, A. oryzae, K. lactis, K. fragilis, which are the main producers of β-galactosidase used in food industry.…”

“…Oxides materials such as alumina, silica, silicated alumina were used for covalent binding of β-galactosidase from K. marxianus and applied in lactose hydrolysis processes. In spite of the immobilisates showed good stability, the immobilisation yields were less than 5% (Di Serio et al 2003). β-Galactosidase from K. fragilis was covalently linked to silanised porous glass beads via amino groups, using glutaraldehyde.…”

Novel multienzyme oxidative biocatalyst for lignin bioprocessing

“…About 90% of both enzymes was successfully immobilized respect to starting material, in accord with previously reported results for the chemical immobilization of enzymes on alumina pellets under similar experimental conditions. [50][51][52] Figure 2 shows the immobilization efficiency as evaluated by the residual enzymatic activity after immobilization and coating. Under these conditions it was found always higher for HRP than for laccase.…”