Lactose hydrolysis by immobilized ?-galactosidase on nylon-6: A novel spin-basket reactor

Ortega‐López, Jaime; Morales-Ramos, Lilia H.; Montes, M. C.; Magaña-Plaza, I.

doi:10.1007/bf00153743

Cited by 7 publications

(1 citation statement)

References 12 publications

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“…Microbeads of Nylon-6 were activated with glutaraldehyde, using polyethylenimine (PEI) as a spacer [5,10,11]. The procedure was adapted and optimized for re-INVB as follows: 1.0 g of Nylon-6 microbeads was resuspended in 10 ml of 9.25% (v/v) glutaraldehyde in 200 mM sodium tetraborate, pH 9.3.…”

Section: Preparation Of Nylon-6mentioning

confidence: 99%

Immobilization of the recombinant invertase INVB from Zymomonas mobilis on Nylon-6

Vallejo-Becerra

Vásquez-Bahena

Santiago-Hernández

et al. 2008

J Ind Microbiol Biotechnol

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The recombinant invertase INVB (re-INVB) from Zymomonas mobilis was immobilized on microbeads of Nylon-6, by means of covalent bonding. The enzyme was strongly and successfully bound to the support. The activity of the free and immobilized enzyme was determined, using 10% (w/v) sucrose, at a temperature ranging between 15 and 60 degrees C and a pH ranging between 3.5 and 7. The optimal pH and temperature for the immobilized enzyme were 5.5 and 25 degrees C, respectively. Immobilization of re-INVB on Nylon-6 showed no significant change in the optimal pH, but a difference in the optimal temperature was evident, as that for the free enzyme was shown to be 40 degrees C. The values for kinetic parameters were determined as: 984 and 98 mM for Kappm of immobilized and free re-INVB, respectively. Kappcat values for immobilized and free enzymes were 6.1x10(2) and 1.2x10(4) s(-1), respectively, and immobilized re-INVB showed Vappmax of 158.73 micromol h min(-1) mg(-1). Immobilization of re-INVB on Nylon-6 enhanced the thermostability of the enzyme by 50% at 30 degrees C and 70% at 40 degrees C, when compared to the free enzyme. The immobilization system reported here may have future biotechnological applications, owing to the simplicity of the immobilization technique, the strong binding of re-INVB to the support and the effective thermostability of the enzyme.

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Section: Preparation Of Nylon-6mentioning

confidence: 99%