Lactose-Enhanced Cellulase Production by Microbacterium sp. Isolated from Fecal Matter of Zebra (Equus zebra)

Abstract

A cellulase-producing bacterial strain designated Z5 was isolated from the fecal matter of Zebra (Equus zebra). The strain was identified as Microbacterium sp. on the basis of 16S rDNA sequence analysis. The effect of substrates like CMC, avicel, starch, maltose, sucrose, glucose, fructose, galactose, and lactose on cellulase production was also determined. Lactose as the sole carbon source induced cellulase production in this bacterial strain and a positive synergistic effect of lactose and CMC was also observed with enhancement of 3-4 times in cellulase activity. The optimum cellulase production was recorded with 3% CMC and 1% lactose when added individually in the Omeliansky's medium. The optimum temperature and time for cellulase production by this bacterial strain was 37°C and 10 days, respectively. To our knowledge this is the first report on enhancement of cellulase production by lactose in the Microbacterium sp.

Optimization of Cultural Condition and Synergistic Effect of Lactose with Carboxymethyl Cellulose on Cellulase Production by <i>Bacillus</i> sp. Isolated from Fecal Matter of Elephant (<i>Elephas maximus</i>)

“…This result indicated that Lactose synergistically enhanced the cellulase synthesis. Similar results reported earlier with a fungus Hypocrea jecorina [37] and in bacteria Microbacterium [21]. However, the lase synthesis cannot be induced by galactose or glucose individually ( Table 1).…”

“…Reports from literature suggested that glucose inhibited cellulase synthesis [38] and lactose induces significantly higher cellulase levels than D-galactose in T. reesei [39]. The induction mechanism of cellulase formation is studied in fungus by many workers [40] and in bacteria [21]. We therefore consider that lactose is a water soluble disaccharide in the mixed medium with CMC and was fast taken up by Bacillus sp.…”

Optimization and strain improvement by mutation for enhanced cellulase production by Bacillus sp. (MTCC10046) isolated from cow dung

“…BRC1 and BRC2 (Chellapandi and Himanshu, 2008), A. flavithermus EHP1 (Ibrahim and Ahmed, 2007), Microbacterium sp. (Sadhu et al, 2011), Bacillus sp. (Sadhu et al, 2013).…”

“…(Chellapandi and Himanshu, 2008), Thermomonospora (George et al, 2001), Microbacterium sp. (Sadhu et al, 2011), Streptomyces transformant T3-1 (Jang and Chen, 2003), Streptomyces sp. F2621 (Tuncer et al, 2004), Clostridium papyrosolvens (Thirumale et al, 2001), Acidothermus cellulolyticus (Shiang et al, 1991), Bacillus sp.…”

Characterization of Novel Cellulase-producing Bacteria Isolated From Rotting Wood Samples

“…Other researchers also reported the maximum CMCase production by using lactose as a source of carbon by Microbacterium sp. [45], Aspergillus hortai [46] and Trichoderma reesei [47]. …”