Lactoperoxidase

Naidu, A. S.

doi:10.1201/9781420039368.ch3

Cited by 14 publications

(17 citation statements)

References 98 publications

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“…As opposed to oxidants like H 2 O 2 and superoxide (O 2 Ϫ ), OSCN Ϫ causes no DNA damage and is considered nontoxic for the host cells producing it (43). In fact, the LP system is thought to protect host cells by consuming the potentially harmful H 2 O 2 produced during oxidative burst (19).…”

mentioning

confidence: 99%

CorA Affects Tolerance of Escherichia coli and Salmonella enterica Serovar Typhimurium to the Lactoperoxidase Enzyme System but Not to Other Forms of Oxidative Stress

Sermon

Wevers

Jansen

et al. 2005

Appl Environ Microbiol

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The enzyme lactoperoxidase is part of the innate immune system in vertebrates and owes its antimicrobial activity to the formation of oxidative reaction products from various substrates. In a previous study, we have reported that, with thiocyanate as a substrate, the lactoperoxidase system elicits a distinct stress response in Escherichia coli MG1655. This response is different from but partly overlapping with the stress responses to hydrogen peroxide and to superoxide. In the current work, we constructed knockouts in 10 lactoperoxidase system-inducible genes to investigate their role in the tolerance of E. coli MG1655 to this antimicrobial system. Five mutations resulted in a slightly increased sensitivity, but one mutation (corA) caused hypersensitivity to the lactoperoxidase system. This hypersensitive phenotype was specific to the lactoperoxidase system, since neither the sensitivity to hydrogen peroxide nor to the superoxide generator plumbagin was affected in the corA mutant. Salmonella enterica serovar Typhimurium corA had a similar phenotype. Although corA encodes an Mg 2؉ transporter and at least three other inducible open reading frames belonged to the Mg 2؉ regulon, repression of the Mg stimulon by Mg 2؉ did not change the lactoperoxidase sensitivity of either the wild-type or corA mutant. Prior exposure to 0.3 mM Ni 2؉ , which is also transported by CorA, strongly sensitized MG1655 but not the corA mutant to the lactoperoxidase system. Furthermore, this Ni 2؉ -dependent sensitization was suppressed by the CorA-specific inhibitor Co(III) hexaammine. These results indicate that CorA affects the lactoperoxidase sensitivity of E. coli by modulating the cytoplasmic concentrations of transition metals that enhance the toxicity of the lactoperoxidase system.

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mentioning

confidence: 99%

CorA Affects Tolerance of Escherichia coli and Salmonella enterica Serovar Typhimurium to the Lactoperoxidase Enzyme System but Not to Other Forms of Oxidative Stress

Sermon

Wevers

Jansen

et al. 2005

Appl Environ Microbiol

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“…The most generally prescribed industrial utilization of LP systems is the preservation of raw fresh milk during transportation and storage in dairy plants [92,93]. It received a considerable attention as an optimum indicator of super-pasteurized milk [94].…”

Section: Lactoperoxidasesmentioning

confidence: 99%

Lactation Responses toward Milk Indigenous Enzymes

Hameed¹,

Hussain²,

Akhtar³

2017

Livestock Science

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Milk being a highly nutritious food in its natural form provides energy. There are various factors influencing the composition of milk: breed, stage of lactation, nutritional status, health, and milking intervals. A number of indigenous enzymes present in milk are being affected by stages of lactation period. Their concentration varies during early, mid and late lactation periods. This varied behavior ultimately affects the quality of dairy products. In this chapter, the level of milk enzymes: lipases and esterases, plasmin (PL), plasminogen (PLG) phosphatases (alkaline phosphatase ALP; acid phosphatase (ACP), lysozyme (LZ), lactoperoxidase (LP), xanthine oxidoreductase (XOR), and catalase (CAT) will be reviewed with respect to the stages of lactation periods.

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“…Lactoperoxidase (LP) is a glycoprotein that presents by nature in colostrums and milk (Kussendrager and van Hooijdonk, 2000;Conner et al, 2002). This LP system has been proven to be effective against both grampositive and gram-negative bacteria (Naidu, 2000;Marks et al, 2001). LP system becomes active with the presence of hydrogen peroxide and thiocyanate.…”

Section: Introductionmentioning

confidence: 99%

Use of Hydrogen Peroxide (H2O2) in raw cow's milk preservation

Arefin¹,

Sarker²,

Islam³

et al. 2017

J Adv Vet Anim Res

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Objective: Hydrogen peroxide (H2O2) was used for the activation of lactoperoxidase system on preservation of milk. Materials and methods: Milk samples were collected from Bangladesh Agricultural University dairy farm. The collected milk samples were added with 0.02, 0.04, 0.06, 0.08, 0.10, 0.12 and 0.14% of 10% H2O2 along with control one. All milk samples were kept at room temperature (28-31 0 C). Flavor, clot on boiling (COB), acidity %, and methylene blue reduction (MBR) test were observed every one hour interval. Results: The milk samples were acceptable in terms of flavor up to 18 h at 0.14% H2O2. Similarly, this milk sample took maximum time (19 h) to give COB positive test. Acidity % was within normal range for 0.02, 0.04, 0.06, 0.08, 0.10, 0.12 and 0.14% H2O2 treated milk samples up to 8,9,11,12,12,14,17 and 18 h, respectively. Significant difference (P<0.01) was found among the levels of H2O2 in acidity development at every observation hour except 0 hour. In MBR test, control sample was decolorized at 7 h and H2O2 treated milk samples were decolorized after 8 to 13 h depending on the level of added H2O2. Conclusion: Based on the results, it may be recommended that 0.14% H2O2 is suitable to extend the milk shelf-life where milk cooling facilities are not available.

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Lactoperoxidase

Cited by 14 publications

References 98 publications

CorA Affects Tolerance of Escherichia coli and Salmonella enterica Serovar Typhimurium to the Lactoperoxidase Enzyme System but Not to Other Forms of Oxidative Stress

CorA Affects Tolerance of Escherichia coli and Salmonella enterica Serovar Typhimurium to the Lactoperoxidase Enzyme System but Not to Other Forms of Oxidative Stress

Lactation Responses toward Milk Indigenous Enzymes

Use of Hydrogen Peroxide (H2O2) in raw cow's milk preservation

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