Lactonase SsoPox modulates CRISPR-Cas expression in gram-negative proteobacteria using AHL-based quorum sensing systems

Mion, Sonia; Plener, Laure; Rémy, Benjamin; Daudé, David; Chabrière, Éric

doi:10.1016/j.resmic.2019.06.004

Cited by 17 publications

(16 citation statements)

References 20 publications

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“…SsoPox W263I variant reinforced antibiotics and bacteriophage treatments against P. aeruginosa in vitro and in vivo using an amoeba infection model with Acanthamoeba polyphaga (84) and was further shown to alter the regulation of the CRISPR-Cas system in P. aeruginosa clinical strains, thereby potentially modifying their ability to compete with phages. In fact, this finding is consistent with the previous observation that the CRISPR adaptation system is induced by QS at high population density (85)(86)(87). Finally, this variant also reduced biofilm formation and violacein production and down-regulated CRISPR-Cas genes of C. violaceum, a tropical aquatic bacterium responsible for rare but frequently fatal infections in animals and humans (85,88,89).…”

Section: Human Healthsupporting

confidence: 91%

“…In fact, this finding is consistent with the previous observation that the CRISPR adaptation system is induced by QS at high population density (85)(86)(87). Finally, this variant also reduced biofilm formation and violacein production and down-regulated CRISPR-Cas genes of C. violaceum, a tropical aquatic bacterium responsible for rare but frequently fatal infections in animals and humans (85,88,89). In vivo, SsoPox W263I was also proved to protect rats from P. aeruginosa infection in a pneumonia model.…”

Section: Human Healthsupporting

confidence: 90%

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Engineering acyl-homoserine lactone-interfering enzymes toward bacterial control

Billot

Plener

Jacquet

et al. 2020

Journal of Biological Chemistry

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Enzymes able to degrade or modify acyl-homoserine lactones (AHL) have drawn considerable interest for their ability to interfere with the bacterial communication process referred to as Quorum Sensing (QS). Many proteobacteria use AHL to coordinate virulence and biofilm formation in a cell density-dependent manner, thus AHL-interfering enzymes constitute new promising antimicrobial candidates. Among these, lactonases and acylases, have been particularly studied. These enzymes have been isolated from various bacterial, archaeal or eukaryotic organisms and have been evaluated for their ability to control several pathogens. Engineering studies on these enzymes were carried out and successfully modulated their capacity to interact with specific AHL, increase their catalytic activity and stability or enhance their biotechnological potential. In this review, special attention is paid to the screening, engineering and applications of AHL-modifying enzymes. Prospects and future opportunities are also discussed with view to developing potent candidates for bacterial control.

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Section: Human Healthsupporting

confidence: 91%

Section: Human Healthsupporting

confidence: 90%

Engineering acyl-homoserine lactone-interfering enzymes toward bacterial control

Billot

Plener

Jacquet

et al. 2020

Journal of Biological Chemistry

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“…The regulatory components of the LuxIR-like TCS quorum sensing system in Gram-negative bacteria, named SmaIR in Serratia and LasIR/RhlIR in Pseudomonas aeruginosa, induce the expression of cas operons and CRISPR arrays of multiple CRISPR-Cas systems of type I-E, I-F and III-A, thus affecting both interference and immunity acquisition functions of CRISPR-Cas [ 157 , 158 ]. The link between quorum sensing and CRISPR-Cas regulation was recently further confirmed by studies in P. aeruginosa clinical strains, demonstrating the potential of a quorum quenching enzyme degrading quorum-sensing signaling molecules to modulate CRISPR-Cas expression [ 159 ]. Quorum sensing was shown to act synergistically with another cue, low environmental temperature associated with slow growth, to control CRISPR-Cas function in this pathogen [ 162 ].…”

Section: Srnas Regulating Host-pathogen Interactionsmentioning

confidence: 99%

“…Thus, the regulation of defense systems including CRISPR-Cas on the one hand and new DNA acquisition mechanisms including natural competence systems on the other hand is extremely important for bacterial adaptation to changing environments with fitness cost considerations. Recent studies provided new examples of such regulatory mechanisms involving sRNAs under the control of TCS in major pathogens [ 64 , 157 , 158 , 159 , 160 , 161 ].…”

Section: Srnas Regulating Host-pathogen Interactionsmentioning

confidence: 99%

“…Together with many community-level behaviors, quorum sensing has been recently shown to control CRISPR-Cas-mediated immunity in bacteria, including pathogens [ 157 , 158 , 159 ]. The regulatory components of the LuxIR-like TCS quorum sensing system in Gram-negative bacteria, named SmaIR in Serratia and LasIR/RhlIR in Pseudomonas aeruginosa, induce the expression of cas operons and CRISPR arrays of multiple CRISPR-Cas systems of type I-E, I-F and III-A, thus affecting both interference and immunity acquisition functions of CRISPR-Cas [ 157 , 158 ].…”

Section: Srnas Regulating Host-pathogen Interactionsmentioning

confidence: 99%

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Interplay between Regulatory RNAs and Signal Transduction Systems during Bacterial Infection

Piattelli

Peltier

Soutourina

2020

Genes

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The ability of pathogenic bacteria to stably infect the host depends on their capacity to respond and adapt to the host environment and on the efficiency of their defensive mechanisms. Bacterial envelope provides a physical barrier protecting against environmental threats. It also constitutes an important sensory interface where numerous sensing systems are located. Signal transduction systems include Two-Component Systems (TCSs) and alternative sigma factors. These systems are able to sense and respond to the ever-changing environment inside the host, altering the bacterial transcriptome to mitigate the impact of the stress. The regulatory networks associated with signal transduction systems comprise small regulatory RNAs (sRNAs) that can be directly involved in the expression of virulence factors. The aim of this review is to describe the importance of TCS- and alternative sigma factor-associated sRNAs in human pathogens during infection. The currently available genome-wide approaches for studies of TCS-regulated sRNAs will be discussed. The differences in the signal transduction mediated by TCSs between bacteria and higher eukaryotes and the specificity of regulatory RNAs for their targets make them appealing targets for discovery of new strategies to fight against multi-resistant bacteria.

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Rapid Revealing of Quorum Sensing (QS)-Regulated PLA, Biofilm and Lysine Targets of Lactiplantibacillus plantarum L3

Chai,

Ma,

et al. 2024

Curr Microbiol

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Lactonase SsoPox modulates CRISPR-Cas expression in gram-negative proteobacteria using AHL-based quorum sensing systems

Cited by 17 publications

References 20 publications

Engineering acyl-homoserine lactone-interfering enzymes toward bacterial control

Engineering acyl-homoserine lactone-interfering enzymes toward bacterial control

Interplay between Regulatory RNAs and Signal Transduction Systems during Bacterial Infection

Rapid Revealing of Quorum Sensing (QS)-Regulated PLA, Biofilm and Lysine Targets of Lactiplantibacillus plantarum L3

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