Lactoferrin purification and whey protein isolate recovery from cheese whey using chitosan mini-spheres

Hirsch, Daniela Belén; Álvarez, Lauro Vladimir Valle; Urtasun, Nicolás; Baieli, María Fernanda; Lázaro-Martínez, Juan Manuel; Glisoni, Romina Julieta; Miranda, Magdalena; Cascone, Osvaldo; Wolman, Federico Javier

doi:10.1016/j.idairyj.2020.104764

Cited by 15 publications

(6 citation statements)

References 52 publications

(60 reference statements)

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“… 101 The effect of pH on the LF selectivity has been studied and it has been determined that pH 5 and pH 10 are the optimal conditions for absorbing and desorbing LF, respectively. 102 Other alternative stationary phases have been developed to test the feasibility of isolating LF, such as mixed matrix, 60 monolithic columns, and chromatographic membranes. 62 LF was isolated from sweet whey in a recent study using MF and UF followed by expanded-bed chromatography based on cationic exchange.…”

Section: Separation and Purification Technologies Of Milk Bioactivesmentioning

confidence: 99%

Bioactives in bovine milk: chemistry, technology, and applications

et al. 2021

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The significance of dairy in human health and nutrition is gaining significant momentum as consumers continue to desire wholesome, nutritious foods to fulfill their health and wellness needs. Bovine milk not only consists of all the essential nutrients required for growth and development, it also provides a broad range of bioactive components that play an important role in managing human homeostasis and immune function. In recent years, milk bioactives, including α-lactalbumin, lactoferrin, glycomacropeptide, milk fat globule membrane, and milk oligosaccharides, have been intensively studied because of their unique bioactivity and functionality. Challenges for the application of these bioactive components in food and pharmaceutical formulations are associated with their isolation and purification on an industrial scale and also with their physical and chemical instability during processing, storage, and digestion. These challenges can be overcome by advanced separation techniques and sophisticated nano- or micro-encapsulation technologies. Current knowledge about the chemistry, separation, and encapsulation technology of major bioactives derived from bovine milk and their application in the food industry is reviewed here.

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Section: Separation and Purification Technologies Of Milk Bioactivesmentioning

confidence: 99%

Bioactives in bovine milk: chemistry, technology, and applications

et al. 2021

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“…Fractogel TMAE [22] sc pDNA Capto TM adhere resin [23] Lactoferrin Sulfanilic acid-modified chitosan mini-spheres [24] Nucleic Acids Sepharose CL-6B treated with 1,4-butanediol diglycidyl ether [25] mcDNA Sephacryl S-1000 SF matrix [26] mcDNA Cadaverine modified monolith [27] pre-miRNA-29 L-arginine-Sepharose 4B gel [28] sc pDNA…”

Section: Adenoviral Vectormentioning

confidence: 99%

“…The authors isolated the sc pDNA isoform from other impurities, such as RNA and open circular pDNA isoform, with a recovery yield of over 90%. In a different study, Hirsch et al [ 24 ] evaluated a multi-modal chitosan-based chromatographic support for isolating lactoferrin directly from cheese whey without a previous clarification treatment. With sulfanilic acid-modified chitosan mini-spheres, the authors could capture 68% of lactoferrin, with a purity degree of 70%.…”

Section: Biomolecules Purification By Preparative Chromatographymentioning

confidence: 99%

Supported Ionic Liquids Used as Chromatographic Matrices in Bioseparation—An Overview

et al. 2022

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Liquid chromatography plays a central role in biomanufacturing, and, apart from its use as a preparative purification strategy, either in biopharmaceuticals or in fine chemicals industries, it is also very useful as an analytical tool for monitoring, assessing, and characterizing diverse samples. The present review gives an overview of the progress of the chromatographic supports that have been used in the purification of high-value products (e.g., small molecules, organic compounds, proteins, and nucleic acids). Despite the diversity of currently available chromatographic matrices, the interest in innovative biomolecules emphasizes the need for novel, robust, and more efficient supports and ligands with improved selectivity. Accordingly, ionic liquids (ILs) have been investigated as novel ligands in chromatographic matrices. Given herein is an extensive review regarding the different immobilization strategies of ILs in several types of supports, namely in silica, Sepharose, and polymers. In addition to depicting their synthesis, the main application examples of these supports are also presented. The multiple interactions promoted by ILs are critically discussed concerning the improved selectivity towards target molecules. Overall, the versatility of supported ILs is here considered a critical point to their exploitation as alternatives to the more conventional liquid chromatographic matrices used in bioseparation processes.

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“…An aptamer affinity column combined with high-performance liquid chromatography and novel multimodal chitosan mini-spheres were used for the purification of Lf from cheese whey without any pretreatment. 17,18 These developed processes and matrices offer potential application in industry.…”

Section: Purification From Milkmentioning

confidence: 99%