Lactoferrin in Relation to Biological Functions and Applications: A Review

El-Loly, M. M.; Mahfouz, M. B.

doi:10.3923/ijds.2011.79.111

Cited by 36 publications

(13 citation statements)

References 100 publications

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“…In both cLFchimera*LPS and cLFchimera*DNA interactions the same residues of peptide acted as active compounds. Based on our contribution analysis, LYS 5,9,13,18,21,24,25,34,35 and ARG16,22,27 have the negative binding energy, therefore, they are the key residues for these complex formations. According to different charge between peptide and targets these results are rational.…”

Section: Discussionmentioning

confidence: 93%

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Generation of an engineered food-grade Lactococcus lactis strain for production of an antimicrobial peptide: in vitro and in silico evaluation

et al. 2020

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Background: Foodborne pathogens and their biofilms are considered as one of the most serious problems in human health and food industry. Moreover, safety of foods is a main global concern because of the increasing use of chemical food additives. Ensuring food safety enhances interest in discovery of new alternative compounds such as antimicrobial peptides (AMPs), which can be used as bio-preservatives in the food industry. In this study, the most important antimicrobial peptides of camel milk lactoferrin (lactoferrampin and lactoferricin) were recombinantly expressed in the form of chimeric peptide (cLFchimera) in a food-grade L. lactis strain. P170 expression system was used to express secreted cLFchimera using pAMJ1653 expression vector which harbors a safe (non-antibiotic) selectable marker. Results: Peptide purification was carried out using Ni-NTA agarose column from culture medium with concentration of 0.13 mg/mL. The results of disk diffusion test revealed that cLFchimera had considerable antimicrobial activity against a number of major foodborne bacteria. Furthermore, this chimeric peptide showed strong and weak inhibitory effect on biofilm formation against P. aeruginosa, S. aureus E. faecalis, and E. coli, respectively. Antioxidant activity and thermal stability of the chimeric peptide was determined. The results showed that cLFchimera had antioxidant activity (IC 50 : 310 μ/mL) and its activity was not affected after 40 min of boiling. Finally, we evaluated the interaction of the peptide with LPS and DNA in bacteria using molecular dynamic simulation as two main intra and extra cellular targets for AMPs, respectively. Our in silico analysis showed that cLFchimera had strong affinity to both of these targets by positive charged residues after 50 ns molecular dynamic simulation. Conclusions: Overall, the engineered food-grade L. lactis generated in the present study successfully expressed a secreted chimeric peptide with antimicrobial properties and could be considered as a promising bio-preservative in the food industry.

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Section: Discussionmentioning

confidence: 93%

“…The thermal processing method is usually used for different stages of the food industry. For example, it can be important in sterilizing high resistance proteins such as lactoferrin [18]. The thermal stability of lactoferrin has already been reported [13].…”

Section: Discussionmentioning

confidence: 99%

Generation of an engineered food-grade Lactococcus lactis strain for production of an antimicrobial peptide: in vitro and in silico evaluation

et al. 2020

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“…The production of ROS in semen can be triggered by Fe 2+ and transition ion through the Fenton reaction which leads to lipid and protein oxidation (Aitken, Harkiss, & Buckingham, 1993;Sadrzadeh, Graf, Panter, Hallaway, & Eaton, 1984). The lactotransferrin also plays a natural antioxidant role and therefore can decrease the amount of free iron available to initiate lipid or protein oxidation (El-Loly & Mahfouz, 2011).…”

Section: Discussionmentioning

confidence: 99%

Lipid and protein oxidation levels in spermatozoa and seminal plasma of Asian Elephants (Elephas maximus) and their relationship with semen parameters

Satitmanwiwat

Promthep

Buranaamnuay

et al. 2017

Reprod Domestic Animals

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Peroxidation damage to spermatozoa and seminal plasma has an important role in sperm quality. Thus, the objective of this study was to determine the levels of lipid and protein oxidation in spermatozoa and seminal plasma of Asian elephants (Elephas maximus) with varying percentage of progressive motility. Lipid and protein oxidation was measured by the thiobarbituric acid-reactive species (TBARS) assay and the 2, 4-dinitrophenylhydrazine (DNPH) carbonyl groups assay, respectively. Fresh semen samples were collected from Asian elephants and classified according to the percentage of motile spermatozoa into good (>60%) and poor (≤20%) motility. Results revealed that seminal plasma malondialdehyde (MDA) and seminal plasma protein carbonyls (PCs) were significantly higher in poor motility than in good motility (p < .05). The MDA and PC levels in seminal plasma were negatively correlated with the percentages of progressive motility (p < .05). In addition, the negative correlation between sperm concentration and seminal plasma MDA level was investigated (p < .05). The sperm viability was also negatively correlated with sperm PC level (p < .05). This study indicated that lipid and protein oxidation has deleterious effect on semen quality of Asian elephants.

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“…It is particularly resistant to proteolytic degradation in the alimentary tract. Lf‐derived peptides possess potent antibacterial activities …”

Section: Whey Proteins As a Source Of Bioactive Componentsmentioning

confidence: 99%

Role of whey proteins in combating geriatric disorders

et al. 2013

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Ageing in humans is usually accompanied by deterioration in physical fitness and increased susceptibility to disease, adding to the economic burden of the person concerned. Inadequate food intake, reduced desire to eat, decline in food digestion or absorption, compromise in nutritional metabolic pathways, poor dental hygiene or preponderance of chronic diseases, which are commonly found in the elderly, can lead to an increased risk of under‐nutrition. Adequate dietary nutrition has an undisputed role in healthy ageing. Among the various nutrients, proteins play an important role in geriatric health. This review emphasizes the nutritional aspects of milk‐based whey proteins and their role in tackling health problems in the geriatric population. Nutritionally rich whey protein constituents such as β‐lactoglobulin, α‐lactalbumin, bovine serum albumin, lactoferrin and proteose peptone have all been reported to have a plethora of functional roles in various biological processes and organ systems in geriatrics and thus help in the management of geriatric health problems through proper nutrition. © 2013 Society of Chemical Industry

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Lactoferrin in Relation to Biological Functions and Applications: A Review

Cited by 36 publications

References 100 publications

Generation of an engineered food-grade Lactococcus lactis strain for production of an antimicrobial peptide: in vitro and in silico evaluation

Generation of an engineered food-grade Lactococcus lactis strain for production of an antimicrobial peptide: in vitro and in silico evaluation

Lipid and protein oxidation levels in spermatozoa and seminal plasma of Asian Elephants (Elephas maximus) and their relationship with semen parameters

Role of whey proteins in combating geriatric disorders

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