Lactoferrin: from the structure to the functional orchestration of iron homeostasis

Ianiro, Giusi; Rosa, Luigi; Patti, Maria Carmela Bonaccorsi di; Valenti, Piera; Musci, Giovanni; Cutone, Antimo

doi:10.1007/s10534-022-00453-x

Cited by 9 publications

(14 citation statements)

References 191 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since lactoferrin has similar antimicrobial activity to its AMP derivatives [ 15 ], it was also used in further wet experiments studying the catalytic and physical–chemical properties of combinations of the AMP with His 6 -OPH.…”

Section: Resultsmentioning

confidence: 99%

“…As a result of careful studies conducted over many years of mainly antimicrobial properties and the mechanism of action of lactoferrin isolated from various sources (human, cow, buffalo, deer, ship, mouse etc.) [ 13 , 14 , 15 , 16 ], it was determined that both lactoferrin itself and its polypeptide derivatives (lactoferricin (Lfcin), lactoferampin (Lfampin) and the short peptide Lf(1-11)) of bovine origin in most cases exhibit significantly higher antimicrobial activity against various yeasts, Gram-positive (G(+)) and Gram-negative (G(−)) bacterial cells ( Table 1 ). At the same time, the highest antimicrobial activity is noted for bovine Lfcin.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Synergistic Antimicrobial Action of Lactoferrin-Derived Peptides and Quorum Quenching Enzymes

Aslanli

Domnin

Stepanov

et al. 2023

IJMS

View full text Add to dashboard Cite

Combined use of various antimicrobial peptides (AMPs) with enzymes that hydrolyze the signaling molecules of the resistance mechanism of various microorganisms, quorum sensing (QS), to obtain effective antimicrobials is one of the leading approaches in solving the antimicrobial resistance problem. Our study investigates the lactoferrin-derived AMPs, lactoferricin (Lfcin), lactoferampin and Lf(1-11), as potential partners for combination with enzymes hydrolyzing lactone-containing QS molecules, the hexahistidine-containing organophosphorus hydrolase (His6-OPH) and penicillin acylase, to obtain effective antimicrobial agents with a scope of practical application. The possibility of the effective combination of selected AMPs and enzymes was first investigated in silico using molecular docking method. Based on the computationally obtained results, His6-OPH/Lfcin combination was selected as the most suitable for further research. The study of physical–chemical characteristics of His6-OPH/Lfcin combination revealed the stabilization of enzymatic activity. A notable increase in the catalytic efficiency of action of His6-OPH in combination with Lfcin in the hydrolysis of paraoxon, N-(3-oxo-dodecanoyl)-homoserine lactone and zearalenone used as substrates was established. Antimicrobial efficiency of His6-OPH/Lfcin combination was determined against various microorganisms (bacteria and yeasts) and its improvement was observed as compared to AMP without enzyme. Thus, our findings demonstrate that His6-OPH/Lfcin combination is a promising antimicrobial agent for practical application.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Synergistic Antimicrobial Action of Lactoferrin-Derived Peptides and Quorum Quenching Enzymes

Aslanli

Domnin

Stepanov

et al. 2023

IJMS

View full text Add to dashboard Cite

show abstract

“…Conversely, it has been reported that human transferrin and LF could modulate expression of streptococcal pyrogenic exotoxins (Spes) [ 117 ]. Down-regulation of SpeB may favor bacterial growth, preserving the integrity of M proteins and superantigens [ 118 , 119 ].…”

Section: Natural and Biological Products As An Alternative Treatment ...mentioning

confidence: 99%

Current Views about the Inflammatory Damage Triggered by Bacterial Superantigens and Experimental Attempts to Neutralize Superantigen-Mediated Toxic Effects with Natural and Biological Products

Santacroce,

Topi,

Charitos

et al. 2024

Pathophysiology

View full text Add to dashboard Cite

Superantigens, i.e., staphylococcal enterotoxins and toxic shock syndrome toxin-1, interact with T cells in a different manner in comparison to conventional antigens. In fact, they activate a larger contingent of T lymphocytes, binding outside the peptide-binding groove of the major histocompatibility complex class II. Involvement of many T cells by superantigens leads to a massive release of pro-inflammatory cytokines, such as interleukin (IL)-1, IL-2, IL-6, tumor necrosis factor-alpha and interferon-gamma. Such a storm of mediators has been shown to account for tissue damage, multiorgan failure and shock. Besides conventional drugs and biotherapeutics, experiments with natural and biological products have been undertaken to attenuate the toxic effects exerted by superantigens. In this review, emphasis will be placed on polyphenols, probiotics, beta-glucans and antimicrobial peptides. In fact, these substances share a common functional denominator, since they skew the immune response toward an anti-inflammatory profile, thus mitigating the cytokine wave evoked by superantigens. However, clinical applications of these products are still scarce, and more trials are needed to validate their usefulness in humans.

show abstract

“…Lactoferrin (LF) is an iron-binding glycoprotein present in bodily fluids such as mammalian breast milk, saliva, tears, and nasal secretions, exhibiting various biological functions [ 1 , 2 , 3 ]. The quantity of saliva and its components are important for maintaining oral health.…”

Section: Introductionmentioning

confidence: 99%

Effects of Lactoferrin on Oral and Throat Conditions under Low Humidity Environments: A Randomized, Double-Blind, and Placebo-Controlled Crossover Trial

Kubo,

Oda,

Tanaka

et al. 2023

Nutrients

View full text Add to dashboard Cite

To evaluate the effects of a single ingestion of bovine lactoferrin (bLF) on oral and throat conditions under a low-humidity environment. A randomized, double-blind, 2-sequence, 2-treatment, and 2-period placebo-controlled crossover trial was conducted. Healthy adult subjects orally ingested bLF dissolved in water, or placebo water, followed by exposure to low humidity (20 °C, 20% relative humidity (RH)) for 2 h. The primary endpoint was subjective oral and throat discomfort assessed by a visual analog scale (VAS), which positively correlated with the discomfort. Secondary endpoints were unstimulated whole salivary flow rate (UWSFR) and salivary immunoglobulin A (IgA) secretion rate. Overall, 40 subjects were randomly assigned to two sequences (20 each) and 34 were analyzed. The VAS values for oral and throat discomfort in the bLF treatment were significantly lower than in the placebo treatment, whereas UWSFR and IgA secretion rates were comparable between the two treatments. Adverse drug reactions were not observed. Subjective oral and throat discomfort associated with low humidity is suppressed by a single ingestion of bLF. Our findings demonstrate the novel use of bLF in a clinical situation that leverages its unique characteristics.

show abstract

Lactoferrin: from the structure to the functional orchestration of iron homeostasis

Cited by 9 publications

References 191 publications

Synergistic Antimicrobial Action of Lactoferrin-Derived Peptides and Quorum Quenching Enzymes

Synergistic Antimicrobial Action of Lactoferrin-Derived Peptides and Quorum Quenching Enzymes

Current Views about the Inflammatory Damage Triggered by Bacterial Superantigens and Experimental Attempts to Neutralize Superantigen-Mediated Toxic Effects with Natural and Biological Products

Effects of Lactoferrin on Oral and Throat Conditions under Low Humidity Environments: A Randomized, Double-Blind, and Placebo-Controlled Crossover Trial

Contact Info

Product

Resources

About