Lactoferrin Concentration During Involution of the Bovine Mammary Gland

Welty, Francine K.; Smith, Kenneth L.; Schanbacher, F.L.

doi:10.3168/jds.s0022-0302(76)84188-4

Cited by 107 publications

(74 citation statements)

References 17 publications

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“…Other alveoli had degenerated, such that the lumina were barely distinguishable and contained sloughed cells. (Goodman & Schanbacher, 1991), and consistent with increased secretion of this protein during the dry period (Welty, Smith & Schanbacher, 1976 (Fig. 3) indicating that, as in latelactating goats (Quarrie et al 1994), bovine mammary cells are removed by apoptosis in declining lactation.…”

Section: Dna Analysismentioning

confidence: 62%

Programmed cell death in bovine mammary tissue during lactation and involution

Wilde¹,

Addey²,

Li³

et al. 1997

Experimental Physiology

121

119

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SUMMARYCessation of milk removal causes mammary tissue involution, which in rodents is characterized by extensive tissue degeneration and loss of the majority of luminal epithelial cells by apoptosis. In contrast, bovine mammary tissue shows little histological evidence of tissue remodelling between lactations. In this study, we combined histology with molecular biology to examine the cellular and molecular changes in bovine mammary tissue on cessation of milking. Oligonucleosomal laddering of genomic DNA extracted from lactating tissue indicated that a proportion of cells were dying by apoptosis. This was confirmed by terminal deoxynucleotide transferase-mediated deoxyuridine nick end-labelling of apoptotic cells in lactating tissue sections (TUNEL). One week after cessation of milking, a-lactalbumin and a,5-casein messenger RNA (mRNA) abundance had decreased by 99 and 85 %, respectively, whereas lactoferrin mRNA had increased 20-fold. Drying off was also accompanied by an increase in oligonucleosomal laddering of genomic DNA, and by an increase in the proportion of TUNEL-positive cells, which were localized preferentially in regions where alveolar structure had deteriorated. Therefore, termination of lactation was associated with partial loss of the mammary cell population and dedifferentiation of the remainder.

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Section: Dna Analysismentioning

confidence: 62%

Programmed cell death in bovine mammary tissue during lactation and involution

Wilde¹,

Addey²,

Li³

et al. 1997

Experimental Physiology

121

119

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“…LF expression was shown to be significantly associ ated with the lactation stage (r = 0.557) and daily milk production (r = -0.472) (Cheng et al, 2008). Its concentration was shown to increase substantially (up to 100 mg/mL) during mammary gland involution (Welty et al, 1976). In the present study, LF may represent a positive acute-phase protein that is upregulated during inflammatory conditions such as diabetes.…”

Section: Discussionmentioning

confidence: 73%

Comparative proteomic changes of differentially expressed whey proteins in clinical mastitis and healthy yak cows

Li¹,

Ding²,

Wan³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Under the traditional grazing system on the QinghaiTibetan Plateau, the amount of milk in domesticated yak (Bos grunniens) with clinical mastitis decreases and the milk composition is altered. To understand the mechanisms of mammary gland secreted milk and disease infection, changes in the protein composition of milk during clinical mastitis were investigated using a proteomic approach. Milk whey from yak with clinical mastitis was compared to whey from healthy animals with two-dimensional gel electrophoresis using a mass spectrometer. Thirteen protein spots were identified to be four differentially expressed proteins. Increases in the concentrations of proteins of blood serum origin, including lactoferrin, were identified in mastitic whey compared to normal whey, while concentrations of the major whey proteins, casocidin-I, a-lactalbumin, and b-lactoglobulin, were downregulated in mastitic whey. These results indicated significant differences in protein expression between healthy yaks and those with clinical mastitis, and they may provide valuable information for finding new regulation markers and potential protein targets for the treatment of mastitis.

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“…Lactoferrin was shown to be significantly associated with the stage of lactation (r = 0.557) and daily milk production (r = -0.472) (Cheng et al, 2008). Its concentration increased many times (even to 100 mg/ml) during mammary gland involution (Welty et al, 1976).…”

Section: Lactoferrin In Different Speciesmentioning

confidence: 99%

Lactoferrin: a review

Adlerova¹,

Bartošková²,

Faldyna³

2008

Vet. Med.

261

217

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This review discusses the biological properties of the glycoprotein lactoferrin. Lactoferrin has been identified in secretions from exocrine glands and in specific granules of neutrophils. After degranulation, neutrophils become the main source of lactoferrin in blood plasma. Lactoferrin possesses various biological functions, including roles in iron metabolism, cell proliferation and differentiation, and antibacterial, antiviral, and antiparasitic activity. Many of these functions do not appear to be connected with its iron binding ability. Of late, lactoferrin concentrations have been measured mostly in humans but also in some other species. However, the relationship between its concentration and physiological or pathological effects on body functions is not yet well characterised.

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Lactoferrin Concentration During Involution of the Bovine Mammary Gland

Cited by 107 publications

References 17 publications

Programmed cell death in bovine mammary tissue during lactation and involution

Programmed cell death in bovine mammary tissue during lactation and involution

Comparative proteomic changes of differentially expressed whey proteins in clinical mastitis and healthy yak cows

Lactoferrin: a review

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