Lactoferrin can supply iron for the growth of

Miller-Catchpole, Robin; Kot, Eva; Haloftis, George; Furmanov, Sergey Mikhailovich; Bezkorovainy, Anatoly

doi:10.1016/s0271-5317(96)00252-7

Cited by 19 publications

(18 citation statements)

References 14 publications

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“…Bifidobacteria, one of the predominant bacterial groups that exist in the human gut and play important roles in maintaining human health throughout the life span of the individual [10], are appeared to require iron for their growth and apparently shown to produce no siderophores [11]. Studies also have shown that Lf may indeed provide iron to bifidobacteria [12]. In contrast, Petschow et al [13] suggested that growth promotion of Bifidobacterium spp.…”

Section: Introductionmentioning

confidence: 99%

Bovine lactoferrin region responsible for binding to bifidobacterial cell surface proteins

et al. 2009

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Bovine lactoferrin (bLf) is a multifunctional iron-binding glycoprotein secreted mainly in milk and other secretory fluids. Bovine lactoferrin is reported to promote the growth of bifidobacteria and binding of bLf to bifidobacteria cell is thought to be involved. After separation of bLf half molecule and extraction of surface proteins from bifidobacteria, binding profiles were observed by immunoblotting. No binding was appeared when bLf C-lobe was being reacted with cell surface proteins on PVDF membrane. Conversely, a 50-kDa band was appeared when reacted with either intact bLf or nicked bLf. This result strongly suggests that binding region could be N-lobe.Moreover, blot, probed with nicked bLf, reacted with anti-lactoferricin antibody also produced a 50-kDa band that indicates the binding occurred at lactoferricin region of bLf molecule. Interestingly, despite absence of binding, bLf C-lobe can stimulate the growth of bifidobacteria.

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Section: Introductionmentioning

confidence: 99%

Bovine lactoferrin region responsible for binding to bifidobacterial cell surface proteins

et al. 2009

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“…Thus, it is quite likely that Lf has the ability to capture iron from the environment and then transfer it to the bifidobacteria for their growth. Studies have shown that Lf may indeed provide iron to bifidobacteria (18). In contrast, Petschow et al (24) suggested that growth of Bifidobacterium spp.…”

Section: Resultsmentioning

confidence: 97%

“…Lf has been indicated as a natural candidate for the growth of bifidobacteria, one of the predominant bacterial groups that exists in the human gut and plays important roles in maintaining human health throughout the life span of the individual (19). Several studies in vitro (13,14,18,23,24) and in vivo (12) have shown that Lf has the ability to promote the growth of bifidobacteria. Some researchers have also conducted clinical trials to study the effect of bovine Lf (bLf) on the growth of bifidobacteria.…”

Section: Introductionmentioning

confidence: 99%

Visualization of Bovine Lactoferrin Binding to Bifidobacteria

Rahman

Kim

Ito

et al. 2007

Bioscience Microflora

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In the present study, binding of bovine lactoferrin to bifidobacteria was demonstrated. This is the first report showing the binding of bovine lactoferrin to Bifidobactesrium spp. under a transmission electronic microscope using biotinylated bovine lactoferrin and gold-conjugated streptavidin. In addition, we confirmed that bovine lactoferrinbinding protein exists on the surface of bifidobacteria.

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“…was inhibited to a greater extent than with 76% iron‐saturated Lf. Miller‐Catchpole et al. (1997) also observed an inhibitory effect on the growth of B. breve by iron‐free human Lf, while growth was stimulated by the iron‐saturated form.…”

Section: Resultsmentioning

confidence: 99%

“…Several studies in vitro (Petschow & Talbott, 1991; Saito et al. , 1996; Miller‐Catchpole et al. , 1997; Petschow et al.…”

Section: Introductionmentioning

confidence: 99%

Screening of Bifidobacterium spp. based on in vitro growth responses to bovine lactoferrin

Rahman

Kim

Kumura

et al. 2010

Int J of Food Sci Tech

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In the present study, we investigated the in vitro growth responses of fourteen strains of four Bifidobacterium spp. (Bifidobacterium infantis, B. breve, B. bifidum, B. longum) against bovine lactoferrin (bLf) at various concentrations. Bacterial strains were grown in deMan, Rogosa and Sharpe (MRS) broth with or without bLf, and growth was monitored by measuring absorbance at 660 nm. A dose-dependent and straindependent growth response was observed. Bifidobacterium spp. were ranked into high, medium and low according to their calculated relative growth response levels against lactoferrin (Lf). Strains showed better growth responses against holo-type Lf. However, no inhibitory effects at high concentrations (4 mg mL )1 ) or with apo-type Lf were observed. These results strongly suggest that the growth response of Bifidobacterium spp. against bLf may be a selection criterion for their use in fermented products. In addition, use of holo-type Lf in fermented food products may be more effective for probiotic growth, and could also be used as a source of iron to the host.

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Lactoferrin can supply iron for the growth of

Cited by 19 publications

References 14 publications

Bovine lactoferrin region responsible for binding to bifidobacterial cell surface proteins

Bovine lactoferrin region responsible for binding to bifidobacterial cell surface proteins

Visualization of Bovine Lactoferrin Binding to Bifidobacteria

Screening of Bifidobacterium spp. based on in vitro growth responses to bovine lactoferrin

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