Lactoferrin and lactoferrin chimera inhibit damage caused by enteropathogenic Escherichia coli in HEp-2 cells

Flores-Villaseñor, Héctor; Canizalez-Román, Adrian; Garza, Mireya de la; Nazmi, Kamran; Bolscher, Jan G. M.; León-Sicairos, Nidia

doi:10.1016/j.biochi.2012.05.011

Cited by 17 publications

(7 citation statements)

References 43 publications

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“…In this context, BholoLf reduced the adhesion of enterotoxigenic E. coli (ETEC) to the JTC17 cell line and different portions of mouse intestine [56]. In addition, [57] reported that BholoLf (30% iron saturation) suppressed the adhesion of EPEC to HEp-2 cells by 88%. Other experiments are necessary to demonstrate the role that MhHM (OmpA) and MhMP (porin) play in M. haemolytica in their interaction with BholoLf.…”

Section: Discussionmentioning

confidence: 99%

Two outer membrane proteins are bovine lactoferrin-binding proteins in Mannheimia haemolytica A1

Samaniego-Barrón

Luna-Castro

Piña-Vázquez

et al. 2016

Vet Res

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Mannheimia haemolytica is a Gram negative bacterium that is part of the bovine respiratory disease, which causes important economic losses in the livestock industry. In the present work, the interaction between M. haemolytica A1 and bovine lactoferrin (BLf) was studied. This iron-chelating glycoprotein is part of the mammalian innate-immune system and is present in milk and mucosal secretions; Lf is also contained in neutrophils secondary granules, which release this glycoprotein at infection sites. It was evidenced that M. haemolytica was not able to use iron-charged BLf (BholoLf) as a sole iron source; nevertheless, iron-lacked BLf (BapoLf) showed a bactericidal effect against M. haemolytica with MIC of 4.88 ± 1.88 and 7.31 ± 1.62 μM for M. haemolytica strain F (field isolate) and M. haemolytica strain R (reference strain), respectively. Through overlay assays and 2-D electrophoresis, two OMP of 32.9 and 34.2 kDa with estimated IP of 8.18 and 9.35, respectively, were observed to bind both BapoLf and BholoLf; these OMP were identified by Maldi-Tof as OmpA (heat-modifiable OMP) and a membrane protein (porin). These M. haemolytica BLf binding proteins could be interacting in vivo with both forms of BLf depending on the iron state of the bovine.Electronic supplementary materialThe online version of this article (doi:10.1186/s13567-016-0378-1) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Two outer membrane proteins are bovine lactoferrin-binding proteins in Mannheimia haemolytica A1

Samaniego-Barrón

Luna-Castro

Piña-Vázquez

et al. 2016

Vet Res

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“…The underlying mechanism may be related to the type β secretion of bacteria. [9] Other data indicated that the production of tumor-necrosis factor-α (TNF-α), IL-6, IL-1B participated in the process of anti-inflammation. [10] The possible mechanism was that ALA produced by the body in immune disorder reacted with LF, which led to the inhibition of LF functions and increase of proinflammation factors, followed by the injury of liver after the exacerbation of inflammation.…”

Section: Discussionmentioning

confidence: 99%

Detection of Anti-Lactoferrin Antibodies and Anti-Myeloperoxidase Antibodies in Autoimmune Hepatitis: A Retrospective Study

Tan

Zhang

Peng

et al. 2014

Journal of Immunoassay and Immunochemistry

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Anti-lactoferrin antibodies (ALA) and anti-myeloperoxidase antibodies (AMPA) are specific serological markers for autoimmune hepatitis (AIH). The project aimed to detect ALA and AMPA and explore their clinical significances in AIH patients. 59 AIH patients, 217 non AIH patients, and 50 healthy controls were enrolled in this study. ALA and AMPA were detected by ELISA. Antineutropil cytoplasmic antibodies (ANCA) and anti-smooth muscle antibodies (ASMA) were examined by indirect immunofluorescence. Antimitochondrial antibody M2 subtype (AMA-M2), anti-liver kidney microsomal antibody Type 1 (LKM1), anti-liver cytosol antibody Type 1 (LC1), and anti-soluble liver antigen/liver-pancreas antibodies (SLA/LP) were tested by immunoblot. The positivity for ALA was 18.6% in AIH group, only one patient in non-AIH group was positive for ALA; the positivity for AMPA was 59.3% in AIH group, with significant differences (P < 0.01) compared with other groups. The specificities for ALA and AMPA were 99.63% and 97.75%; the sensitivities were 18.64% and 59.32%; and the accuracy rates were 84.97% and 90.80%, respectively. A certain correlation was observed between ALA and SLA/LP, AMPA and ANCA, ASMA in AIH group. ALA and AMPA were associated with AIH, and had high clinical diagnostic value. Co-detection with other relative autoantibodies could play an important role in differential diagnosis of AIH.

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“…Concerning EPEC strains, Lf inhibits the bacterial adherence to human epithelial cells (HeLa and HEp-2) [86,91] and actin polymerization, and therefore prevents the development of the A/E lesion [91]. It has been shown that Lf interacts with the structural subunits of the type III secretion system (TTSS) promoting the degradation of both EspA (the filament-forming subunit) and EspB (the pore-forming subunit) (Table 3) [87].…”

Section: Impairing Virulence By Targeting E Coli Adhesinsmentioning

confidence: 99%

“…It has been shown that Lf interacts with the structural subunits of the type III secretion system (TTSS) promoting the degradation of both EspA (the filament-forming subunit) and EspB (the pore-forming subunit) (Table 3) [87]. In addition, Lf reduces the gene expression of EspA about 50% during EPEC strains infection of epithelial cells [91]. Studies of EHEC strain O157:H7 in animal infection models (BALB/c mice), showed that Lf has a protective effect against gut colonization and the spreading toward kidneys [92].…”

Section: Impairing Virulence By Targeting E Coli Adhesinsmentioning

confidence: 99%

Adhesion of Diarrheagenic Escherichia coli and Inhibition by Glycocompounds Engaged in the Mucosal Innate Immunity

Pereira

Giugliano

2013

Biology

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Escherichia coli colonizes the human intestine shortly after birth, with most strains engaging in a commensal relationship. However, some E. coli strains have evolved toward acquiring genetic traits associated with virulence. Currently, five categories of enteroadherent E. coli strains are well-recognized, and are classified in regard to expressed adhesins and the strategy used during the colonization. The high morbidity associated with diarrhea has motivated investigations focusing on E. coli adhesins, as well on factors that inhibit bacterial adherence. Breastfeeding has proved to be the most effective strategy for preventing diarrhea in children. Aside from the immunoglobulin content, glycocompounds and oligosaccharides in breast milk play a critical role in the innate immunity against diarrheagenic E. coli strains. This review summarizes the colonization factors and virulence strategies exploited by diarrheagenic E. coli strains, addressing the inhibitory effects that oligosaccharides and glycocompounds, such as lactoferrin and free secretory components, exert on the adherence and virulence of these strains. This review thus provides an overview of experimental data indicating that human milk glycocompounds are responsible for the universal protective effect of breastfeeding against diarrheagenic E. coli pathotypes.

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Lactoferrin and lactoferrin chimera inhibit damage caused by enteropathogenic Escherichia coli in HEp-2 cells

Cited by 17 publications

References 43 publications

Two outer membrane proteins are bovine lactoferrin-binding proteins in Mannheimia haemolytica A1

Two outer membrane proteins are bovine lactoferrin-binding proteins in Mannheimia haemolytica A1

Detection of Anti-Lactoferrin Antibodies and Anti-Myeloperoxidase Antibodies in Autoimmune Hepatitis: A Retrospective Study

Adhesion of Diarrheagenic Escherichia coli and Inhibition by Glycocompounds Engaged in the Mucosal Innate Immunity

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