Lactofen induces isoflavone accumulation and glyceollin elicitation competency in soybean

Landini, Serena; Graham, Madge Y.; Graham, Terrence L.

doi:10.1016/s0031-9422(02)00709-4

Cited by 42 publications

(31 citation statements)

References 19 publications

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“…The isoflavone content in soybean seed is influenced by diverse environmental factors including temperature during the seedripening period (Tsukamoto et al 1995), nutritional status of the soil (Vyn et al 2002, Landini et al 2002, fungal infection Graham 1996, Dixon et al 1995) and UV irradiation . In contrast to these environmental factors, little information has been obtained about the genetic regulation mechanisms of the isoflavone content.…”

Section: Introductionmentioning

confidence: 99%

Transgressive segregation of isoflavone contents under the control of four QTLs in a cross between distantly related soybean varieties

et al. 2010

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Soybean (Glycine max (L) Merr.) isoflavones have attracted considerable attention for their diverse effects on human health. To determine the genetic factors that contribute to the high isoflavone contents of the soybean varieties 'Peking' and 'Tamahomare', we conducted QTL analyses for the total content of daidzein derivatives (DAC), genistein derivatives (GEC) and glycitein derivatives (GLC), and for the total content of isoflavones (TIC) using recombinant inbred lines (RILs) derived from the cross between 'Peking' and 'Tamahomare'. Ninety six RILs were planted in Kyoto, Osaka and Nagano in 2003 and in Osaka and Nagano in 2004. Transgressive segregation for TIC was detected in all the environments tested. Composite interval mapping for TIC revealed four QTLs: qIso1, qIso2, qIso3 and qIso4, located on LG-A1 (Chr.5), LG-A2 (Chr.8), LG-C1 (Chr.4) and LG-D2 (Chr.17), respectively. The high-isoflavone alleles were derived from Peking at qIso1 and qIso4 and from Tamahomare at qIso2 and qIso3. Several other groups have already reported the former two QTLs, but qIso2 and qIso3 are new discoveries. Our results indicate that the large variation in TIC measurements observed in the RILs could have resulted from the combined effects of alleles at the four QTLs derived from distantly related varieties.

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Section: Introductionmentioning

confidence: 99%

Transgressive segregation of isoflavone contents under the control of four QTLs in a cross between distantly related soybean varieties

et al. 2010

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“…Subsequently, the soybean isoflavonoid phytoalexin glyceollin was reported to accumulate in lactofen-treated leaves in the field (Nelson et al, 2002). This potential for disease protection invoked our interest, and subsequent work in our laboratory using metabolite profiling showed that lactofen induces multiple and dramatic effects on overall isoflavonoid metabolism in soybean (Landini et al, 2002). First, it induces massive accumulations of several isoflavones, including daidzein and genistein, both of which have been implicated in multiple roles in the deployment of soybean phenylpropanoid defenses (Graham and Graham, 1999).…”

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confidence: 99%

“…In addition, while the induction of glyceollin accumulation per se is not a major effect under laboratory conditions, lactofen activates elicitation competency, i.e. the tissue's capacity for glyceollin accumulation, when challenged with the wall glucan elicitor (WGE) from Phytophthora sojae (Landini et al, 2002). Interestingly, lactofen is unusual in that it predominantly induces the accumulation of free isoflavone aglycones rather than their conjugates (Landini et al, 2002).…”

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The Diphenylether Herbicide Lactofen Induces Cell Death and Expression of Defense-Related Genes in Soybean

Graham

2005

Plant Physiology

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Lactofen belongs to the diphenylether class of herbicides, which targets protoporphyrinogen oxidase, which in turn causes singlet oxygen generation. In tolerant plants like soybean (Glycine max), the chemical nonetheless causes necrotic patches called “bronzing” in contact areas. Here it is shown that such bronzing is accompanied by cell death, which was quantified from digital microscopic images using Assess Software. Cellular autofluorescence accompanied cell death, and a homolog of the cell death marker gene, Hsr203j, was induced by lactofen in treated soybean tissues. Thus, this form of chemically induced cell death shares some hallmarks of certain types of programmed cell death. In addition to the cell death phenotype, lactofen caused enhanced expressions of chalcone synthase and chalcone reductase genes, mainly in the exposed and immediately adjacent (proximal) cells. Furthermore, isoflavone synthase genes, which are wound inducible in soybean, were up-regulated by lactofen in both proximal and distal cell zones in minimally wounded cotyledons and further enhanced in wounded tissues. Moreover, if the wall glucan elicitor from Phytophthora sojae was present during lactofen treatment, the induction of isoflavone synthase was even more rapid. These results are consistent with the fact that lactofen triggers massive isoflavone accumulations and activates the capacity for glyceollin elicitation competency. In addition, lactofen induces late expression of a selective set of pathogenesis-related (PR) protein genes, including PR-1a, PR-5, and PR-10, mainly in treated proximal tissues. These various results are discussed in the context of singlet oxygen-induced responses and lactofen's potential as a disease resistance-inducing agent.

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“…While phytoalexins from the groups of glyceollins and coumestrol have been subject of many investigations [18][19][20][21], especially for their pharmacological effect in humans, there are few such reports for the isoflavones genistein, daidzein and glycitein when pathogen attack is involved [22][23][24]. On the other hand, accumulations of isoflavone conjugates and aglycones in soybean tissues were induced by lactofen, the active ingredient of the soybean disease resistance-inducing herbicide [25]. In order to determine elicitation of phytoalexin isoflavones, some authors exposed soybean plants to various phytopathogens and known elicitors derived from phytopathogens.…”

Section: Discussionmentioning

confidence: 99%

Changes in L-phenylalanine ammonia-lyase activity and isoflavone phytoalexins accumulation in soybean seedlings infected with Sclerotinia sclerotiorum

et al. 2013

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Soybean [Glycine max (L.) Merr.] cultivars (Meli, Alisa, Sava and 1511/99) were grown up to V1 phase (first trifoliate and one node above unifoliate) and then inoculated with Sclerotinia sclerotiorum (Lib.) de Bary under controlled conditions. Changes in L-phenylalanine ammonia-lyase (PAL) activity and isoflavone phytoalexins were recorded 12, 24, 48 and 72 h after the inoculation. Results showed an increase in PAL activity in all four examined soybean cultivars 48 h after the inoculation, being the highest in Alisa (2-fold higher). Different contents of total daidzein, genistein, glycitein and coumestrol were detected in all samples. Alisa and Sava increased their total isoflavone content (33.9% and 6.2% higher than control, respectively) as well as 1511/99, although 48 h after the inoculation its content decreased significantly. Meli exhibited the highest rate of coumestrol biosynthesis (72 h after the inoculation) and PAL activity (48 h after the inoculation). All investigated cultivars are invariably susceptible to this pathogen. Recorded changes could point to possible differences in mechanisms of tolerance among them.

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Lactofen induces isoflavone accumulation and glyceollin elicitation competency in soybean

Cited by 42 publications

References 19 publications

Transgressive segregation of isoflavone contents under the control of four QTLs in a cross between distantly related soybean varieties

Transgressive segregation of isoflavone contents under the control of four QTLs in a cross between distantly related soybean varieties

The Diphenylether Herbicide Lactofen Induces Cell Death and Expression of Defense-Related Genes in Soybean

Changes in L-phenylalanine ammonia-lyase activity and isoflavone phytoalexins accumulation in soybean seedlings infected with Sclerotinia sclerotiorum

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