Lactococcus garvieae strains from yellowtail Seriola quinqueradiata carry different lengths of fimbriae on their cell surface

Hirokawa, Yusuke; Irie, Takeshi; Ooyama, Tsuyoshi; Yasuda, Hiroshi; Nakamura, Atsushi; Jin, Dong Il; Kittigul, Chaivat; Yoshida, Terutoyo

doi:10.1111/j.1444-2906.2004.00834.x

Cited by 3 publications

(8 citation statements)

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“…A capsule present on the cell surface is believed to be one of the virulence factors in L. garvieae (Yoshida et al., 1996, 1997). Hirokawa et al. (2004) reported that the virulent L. garvieae cells isolated from yellowtail had well‐developed capsules, and when these cells were cultured in the presence of fish serum that fimbriae were readily found on the cell surface.…”

Section: Discussionmentioning

confidence: 99%

“…In order to produce the mimic in vivo conditions of the bacteria growth, the bacterial strains (MS93003V and MS93003A) were subcultured three times at 25°C for 48 h in 10 ml of filtered normal serum (0.22 μ m) from yellowtail S. quinqueradiata (Hirokawa et al., 2004). Transmission electron microscopy was used to confirm the presence of the capsule and/or fimbriae on the cell surface, in accordance with the method described by Ooyama et al.…”

Section: Methodsmentioning

confidence: 99%

“…Transmission electron microscopy was used to confirm the presence of the capsule and/or fimbriae on the cell surface, in accordance with the method described by Ooyama et al. (2002) and Hirokawa et al. (2004).…”

Section: Methodsmentioning

confidence: 99%

“…Previous reports (Ooyama et al., 1999, 2002; Hirokawa et al., 2004) described that a formalin‐killed attenuated strain (MS93003A) as well as a formalin‐killed virulent strain (MS93003V) conferred immune protection against capsulated virulent strains in fish. Therefore, the aim of this study was to demonstrate the efficacy of the live L. garvieae MS93003A strain as a live vaccine to protect yellowtail S. quinqueradiata against infection by virulent L. garvieae .…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, the capsule surrounding the bacterial cell is believed to play an important role in the pathogenicity of L. garvieae (Barnes et al., 2002a; Ooyama et al., 2002). Injectable or oral vaccines have been developed recently, and the efficacy of these vaccines for the prevention of L. garvieae infection was demonstrated in Japan (Hirokawa et al., 2004). In fish, although the precise mechanisms of protective immunity against L. garvieae infection remain unknown, humoral immunity in immunized fish is known to play an important role in protection against L. garvieae infection (Barnes et al., 2002b; Ooyama et al., 2002).…”

Section: Introductionmentioning

confidence: 99%

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Application of attenuated Lactococcus garvieae strain lacking a virulence-associated capsule on its cell surface as a live vaccine in yellowtail Seriola quinqueradiata Temminck and Schlegel

et al. 2006

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An attenuated Lactococcus garvieae strain lacking a virulenceassociated capsule on its cell surface was evaluated for application as a live vaccine. The attenuated strain (MS93003A) was obtained from the parent strain (MS93003V), which produced a well-developed capsule, by culturing on an agar medium supplemented with 2,3,5-triphenyltetrazolium chloride. When live cells of L. garvieae (MS93003A) or formalin-killed cells (MS93003A) were used as an injectable vaccine, protection against virulent L. garvieae (MS93003V) was conferred on Seriola quinqueradiata. The MS93003A cells did not recover their virulence even after in vivo passages in fish. MS93003A live cells also conferred long-lasting protective immunity to S. quinqueradiata against virulent L. garvieae infection.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Application of attenuated Lactococcus garvieae strain lacking a virulence-associated capsule on its cell surface as a live vaccine in yellowtail Seriola quinqueradiata Temminck and Schlegel

et al. 2006

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First report on genetic characterization, cell‐surface properties and pathogenicity ofLactococcus garvieae, emerging pathogen isolated from cage‐cultured cobia (Rachycentron canadum)

Rao

Pham

Poudyal

et al. 2021

Transbounding Emerging Dis

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The diseased cage‐cultured cobia (Rachycentron canadum) displayed clinical signs, haemorrhagic eyes, dorsal darkness and gross pathological lesions, enlargement of spleen and liver. Haemorrhages were found in brain, heart and liver with cumulative mortality rates ranging from 20% to 50%. Extensive congestion in the heart, liver, spleen, kidney and brain was observed histopathologically. Epicarditis and meningitis were also revealed in diseased cobia. All isolates recovered from the organs (liver, spleen, head kidney, posterior kidney, brain and muscle) of cobia were found to be gram‐positive, non‐motile, ovoid cocci, short‐chain–forming (diplococci) and α‐haemolytic. The API 32 strep system together with the polymerase chain reaction assay for species‐specific primers (pLG1 and pLG2) and the internal transcribed spacer (ITS) region (G1 and L1 primers) confirmed all four selected isolates as Lactococcus garvieae. Partial 16S rDNA nucleotide sequence (~1,100 bp) of one representative L. garvieae isolate AOD109191 (GenBank accession number, MW328528.1) shared 99.9% identities with the 16S rDNA nucleotide sequence of L. garvieae (GenBank accession numbers: MT604790.1). Transmission electron microscopy (TEM) evaluation of one representative L. garvieae isolate (AOD109191) and the results of multiplex PCR did not reveal the presence of the capsular gene cluster (CGC), thus categorizing the isolate as the KG+ phenotype. Capsule staining and TEM observations confirmed the presence of a hyaluronic acid‐like capsule, a possible virulence factor in KG+ phenotype L. garvieae isolates. The pathogenic potential of the representative isolate (AOD109191) was assessed through intraperitoneal injection challenges in cobia. The gross lesions and histopathological changes found in experimentally infected cobia were similar to those seen in naturally infected fish. This is the first report that confirms L. garvieae‐induced ‘warm water lactococcsis’ can cause outbreaks of diseases in cage‐cultured cobia.

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Pathogenicity and Immunogenicity of Non-agglutinating Lactococcus garvieae with Anti-KG– Phenotype Rabbit Serum in Seriola spp.

et al. 2015

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Lactococcus garvieae strains from yellowtail Seriola quinqueradiata carry different lengths of fimbriae on their cell surface

Cited by 3 publications

References 15 publications

Application of attenuated Lactococcus garvieae strain lacking a virulence-associated capsule on its cell surface as a live vaccine in yellowtail Seriola quinqueradiata Temminck and Schlegel

Application of attenuated Lactococcus garvieae strain lacking a virulence-associated capsule on its cell surface as a live vaccine in yellowtail Seriola quinqueradiata Temminck and Schlegel

First report on genetic characterization, cell‐surface properties and pathogenicity ofLactococcus garvieae, emerging pathogen isolated from cage‐cultured cobia (Rachycentron canadum)

Pathogenicity and Immunogenicity of Non-agglutinating <i>Lactococcus garvieae</i> with Anti-KG<sup>–</sup> Phenotype Rabbit Serum in <i>Seriola</i> spp.

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Lactococcus garvieae strains from yellowtail Seriola quinqueradiata carry different lengths of fimbriae on their cell surface

Cited by 3 publications

References 15 publications

Application of attenuated Lactococcus garvieae strain lacking a virulence-associated capsule on its cell surface as a live vaccine in yellowtail Seriola quinqueradiata Temminck and Schlegel

Application of attenuated Lactococcus garvieae strain lacking a virulence-associated capsule on its cell surface as a live vaccine in yellowtail Seriola quinqueradiata Temminck and Schlegel

First report on genetic characterization, cell‐surface properties and pathogenicity ofLactococcus garvieae, emerging pathogen isolated from cage‐cultured cobia (Rachycentron canadum)

Pathogenicity and Immunogenicity of Non-agglutinating <i>Lactococcus garvieae</i> with Anti-KG<sup>&ndash;</sup> Phenotype Rabbit Serum in <i>Seriola</i> spp.

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Pathogenicity and Immunogenicity of Non-agglutinating <i>Lactococcus garvieae</i> with Anti-KG<sup>–</sup> Phenotype Rabbit Serum in <i>Seriola</i> spp.