Lactococcus cremoris subsp. cremoris FC-fermented milk activates protein synthesis and increases skeletal muscle mass in middle-aged mice

Aoi, Wataru; Iwasa, Masayo; Aiso, Chisato; Tabata, Yoshiyuki; Gotoh, Yayoi; Kosaka, Hideki; Suzuki, Takeshi

doi:10.1016/j.bbrc.2022.04.097

Cited by 4 publications

(5 citation statements)

References 33 publications

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“…18 In addition, Lactococcus cremoris subsp can increase muscle mass in middle-aged mice. 19 However, as the typical bacteria related to pro-inflammatory, Family_XIII_AD3011_group and Collinsela are involved in inflammation-related disorders, including sarcopenia. 20,21 In line with these above studies, our correlation analysis also indicated the potential positive effects of Akkermansia, Lactococcus, and Lactobacillus on muscle mass, muscle strength, and muscle function, and the potential negative effect of Family_XIII_AD3011_group and Collinsela on tmuscle mass, muscle strength, and muscle function.…”

Section: Discussionmentioning

confidence: 99%

“…Akkermansia is positively associated with physical function in patients with sarcopenic cirrhosis 18 . In addition, Lactococcus cremoris subsp can increase muscle mass in middle‐aged mice 19 . However, as the typical bacteria related to pro‐inflammatory, Family_XIII_AD3011_group and Collinsela are involved in inflammation‐related disorders, including sarcopenia 20,21 .…”

Section: Discussionmentioning

confidence: 99%

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Faecal microbiota transplantation from young rats attenuates age‐related sarcopenia revealed by multiomics analysis

Shen

Cheng

et al. 2023

J cachexia sarcopenia muscle

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BackgroundGut microbiota plays a key role in the development of sarcopenia via the ‘gut‐muscle’ axis, and probiotics‐based therapy might be a strategy for sarcopenia. Fecal microbiota transplantation from young donors (yFMT) has attracted much attention because of its probiotic function. However, whether or not yFMT is effective for sarcopenia in old recipients is largely unknown. Thus, we aimed to investigate the effect and mechanism of yFMT on age‐related sarcopenia.MethodsThe fecal microbiota of either young (12 weeks) or old (88 weeks) donor rats was transplanted into aged recipient rats for 8 weeks. Then, muscle mass, muscle strength, muscle function, muscle atrophy, and muscle regeneration capacity were measured. Analysis of fecal 16 s rRNA, serum non‐targeted metabolomic, gut barrier integrity, and muscle transcriptome was conducted to elucidate the interaction between gut microbiota and skeletal muscles.ResultsAs evaluated by magnetic resonance imaging examination, grip strength test (P < 0.01), rotarod test (P < 0.05), and exhaustive running test (P < 0.05), we found that yFMT mitigated muscle mass loss, muscle strength weakness, and muscle function impairment in aged rats. yFMT also countered age‐related atrophy and poor regeneration capacity in fast‐ and slow‐switch muscles, which were manifested by the decrease in slow‐switch myofibres (both P < 0.01) and muscle interstitial fibrosis (both P < 0.05) and the increase in the cross‐section area of myofibres (both P < 0.001), fast‐switch myofibres (both P < 0.01), and muscle satellite cells (both P < 0.001). In addition, yFMT ameliorated age‐related dysbiosis of gut microbiota and metabolites by promoting the production of beneficial bacteria and metabolites—Akkermansia, Lactococcus, Lactobacillus, γ‐glutamyltyrosine, 3R‐hydroxy‐butanoic acid, and methoxyacetic acid and inhibiting the production of deleterious bacteria and metabolites—Family_XIII_AD3011_group, Collinsella, indoxyl sulfate, indole‐3‐carboxilic acid‐O‐sulphate, and trimethylamine N‐oxide. Also, yFMT prevented age‐related destruction of gut barrier integrity by increasing the density of goblet cells (P < 0.0001) and the expression levels of mucin‐2 (P < 0.0001) and tight junctional proteins (all P < 0.05). Meanwhile, yFMT attenuated age‐related impairment of mitochondrial biogenesis and function in fast‐ and slow‐switch muscles. Correlation analysis revealed that yFMT‐induced alterations of gut microbiota and metabolites might be closely related to mitochondria‐related genes and sarcopenia‐related phenotypes.ConclusionsyFMT could reshape the dysbiosis of gut microbiota and metabolites, maintain gut barrier integrity, and improve muscle mitochondrial dysfunction, eventually alleviating sarcopenia in aged rats. yFMT might be a new therapeutic strategy for age‐related sarcopenia.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Faecal microbiota transplantation from young rats attenuates age‐related sarcopenia revealed by multiomics analysis

Shen

Cheng

et al. 2023

J cachexia sarcopenia muscle

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“…Few data are currently available regarding the gut-muscle axis of the most efficient of these bacterial taxa, Lactococcus spp., in converting soy isoflavones into absorbable aglycone forms. However, the administration of Lactococcus cremoris fermented milk to middle aged mice promoted muscle protein synthesis and contributed to improve muscle mass [ 157 ]. Furthermore, the prescription of a high-protein diet targeted against sarcopenia to a group of older women was associated with an increased representation of Lactococcus spp.…”

Section: Interaction Between Phenolic Compounds and Microbiota: Possi...mentioning

confidence: 99%

Accounting Gut Microbiota as the Mediator of Beneficial Effects of Dietary (Poly)phenols on Skeletal Muscle in Aging

et al. 2023

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Sarcopenia, the age-related loss of muscle mass and function increasing the risk of disability and adverse outcomes in older people, is substantially influenced by dietary habits. Several studies from animal models of aging and muscle wasting indicate that the intake of specific polyphenol compounds can be associated with myoprotective effects, and improvements in muscle strength and performance. Such findings have also been confirmed in a smaller number of human studies. However, in the gut lumen, dietary polyphenols undergo extensive biotransformation by gut microbiota into a wide range of bioactive compounds, which substantially contribute to bioactivity on skeletal muscle. Thus, the beneficial effects of polyphenols may consistently vary across individuals, depending on the composition and metabolic functionality of gut bacterial communities. The understanding of such variability has recently been improved. For example, resveratrol and urolithin interaction with the microbiota can produce different biological effects according to the microbiota metabotype. In older individuals, the gut microbiota is frequently characterized by dysbiosis, overrepresentation of opportunistic pathogens, and increased inter-individual variability, which may contribute to increasing the variability of biological actions of phenolic compounds at the skeletal muscle level. These interactions should be taken into great consideration for designing effective nutritional strategies to counteract sarcopenia.

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“…Our group reported that α-hydroxy isocaproic acid, a BCHA, prevents atrophy of C2C12 mouse myotubes due to inflammatory cytokine exposure [99], and BCHAs may be a key ingredient to examine regarding the health benefit of yogurt on skeletal muscle. In a study focusing on the anti-inflammatory effects of polysaccharides produced by lactobacillus fermentation of Caspian yogurt containing rich polysaccharides compared with a normal diet in 18-month-old mice for 8 weeks, yogurt treatment reduced TNFα and increased IGF-1 levels in skeletal muscle, and thereby improved muscle mass accompanying increased phosphorylation of mTORC1 and p70S6K [100].…”

Section: Effects Of Prebiotics and Other Ingredientsmentioning

confidence: 99%

Nutritional Value of Yogurt as a Protein Source: Digestibility/Absorbability and Effects on Skeletal Muscle

Sumi,

Tagawa,

Yamazaki

et al. 2023

Nutrients

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Yogurt is a traditional fermented food that is accepted worldwide for its high palatability and various health values. The milk protein contained in yogurt exhibits different physical and biological properties from those of non-fermented milk protein due to the fermentation and manufacturing processes. These differences are suggested to affect the time it takes to digest and absorb milk protein, which in turn will influence the blood levels of amino acids and/or hormones, such as insulin, and thereby, the rate of skeletal muscle protein synthesis via the activation of intracellular signaling, such as the mTORC1 pathway. In addition, based on the relationship between gut microbiota and skeletal muscle conditions, yogurt, including lactic acid bacteria and its metabolites, has been evaluated for its role as a protein source. However, the substantial value of yogurt as a protein source and the additional health benefits on skeletal muscle are not fully understood. The purpose of this review is to summarize the research to date on the digestion and absorption characteristics of yogurt protein, its effect on skeletal muscle, and the contribution of lactic acid bacterial fermentation to these effects.

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Lactococcus cremoris subsp. cremoris FC-fermented milk activates protein synthesis and increases skeletal muscle mass in middle-aged mice

Cited by 4 publications

References 33 publications

Faecal microbiota transplantation from young rats attenuates age‐related sarcopenia revealed by multiomics analysis

Faecal microbiota transplantation from young rats attenuates age‐related sarcopenia revealed by multiomics analysis

Accounting Gut Microbiota as the Mediator of Beneficial Effects of Dietary (Poly)phenols on Skeletal Muscle in Aging

Nutritional Value of Yogurt as a Protein Source: Digestibility/Absorbability and Effects on Skeletal Muscle

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