Lactobionic and gluconic acid complexes of FeII and FeIII; control of oxidation pathways by an organ transplantation preservant

Isaacson, Yisrael; Chensny, Lara; Shepherd, Rex E.; Zhang, Songsheng; Kortes, Richard A.; John, Kevin D.

doi:10.1016/0162-0134(93)80046-c

Cited by 27 publications

(2 citation statements)

References 83 publications

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“…In the biomedical context, metal-chelating properties are very important for the reduction of oxidative damage to tissues caused by some metal ions, e.g., iron, during the storage and preservation of organs [4]. Additionally, lactobionic acid has been shown to inhibit hypothermia-induced cellular swelling in various tissues; additionally, it does not penetrate cell membranes at low temperatures due to its size and three-dimensional structure [5]. LBA is also widely used in the dermatology and cosmetic industries in preparations designed to treat such diseases as atopic dermatitis, rosacea, and seborrheic dermatitis because it is a weak skin irritant, which is important for preventing skin problems.…”

Section: Introductionmentioning

confidence: 99%

Immobilisation of Cellobiose Dehydrogenase and Laccase on Chitosan Particles as a Multi-Enzymatic System for the Synthesis of Lactobionic Acid

Sulej

Piątek-Gołda

Grąz

et al. 2023

JFB

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Lactobionic acid (LBA) is a bioactive compound that has become increasingly popular in medicine in recent years due to its unique properties. This chemical can be formed via the enzymatic oxidation of lactose using fungal oxidoreductive enzymes. This study aimed to intensify the synthesis of LBA using immobilised enzymes (cellobiose dehydrogenase from Phanerochaete chrysosporium (PchCDH) and laccase from Cerrena unicolor (CuLAC)) on chitosan microspheres. We used three different crosslinking agents: genipin, glutaraldehyde, and polyethyleneimine to activate the chitosan. The FTIR and CellDrop techniques were used to characterise the activated microspheres. Quantitative (HPLC) and qualitative (TLC) methods were used to determine the obtained LBA. The results show that the type of activator used influences the efficiency of the binding of the enzyme to the matrix. Furthermore, the amount of LBA formed depends on the type of system used. The use of a system in which one of the enzymes is immobilised on a PEI-activated carrier (PchCDH) and the other is free (CuLAC) proved to be the most optimal, as it yielded almost 100% conversion of lactose to lactobionic acid. Summarising the data obtained the following: lactobionic acid immobilised on chitosan microspheres has great potential for medical applications.

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Section: Introductionmentioning

confidence: 99%

Immobilisation of Cellobiose Dehydrogenase and Laccase on Chitosan Particles as a Multi-Enzymatic System for the Synthesis of Lactobionic Acid

Sulej

Piątek-Gołda

Grąz

et al. 2023

JFB

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“…There are reports of its beneficial effect when employed in the preservation of transplanted organs at low temperatures (Isaacson et al 1989;Charloux et al 1995), in the reduction of hypothermically induced cell swelling (Shepherd et al 1993) and as an additive to meat products (Nielsen 2009). LBA is a compound obtained from chemical or microbial oxidation of lactose.…”

Section: Introductionmentioning

confidence: 99%

Effects of freezing and the cryoprotectant lactobionic acid in the structure of GlnK protein evaluated by circular dichroism (CD) and isothermal titration calorimetry (ITC)

et al. 2016

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Freezing is a widely applied method in food preservation. The technique has negative effects on sensory and textural properties of some foods. In this study the effects of the freeze-thaw process and lactobionic acid (LBA) as a cryoprotectant on GlnK protein solution were evaluated by circular dichroism (CD) analysis and isothermal titration calorimetry (ITC). The freeze-thaw cycles caused changes in GlnK conformation and interactions with small ligands (adenosine triphosphate, ATP). CD assay demonstrated changes in the molar ellipticity values of the samples subjected to freezing, indicating conformational changes to the GlnK protein. Additionally, ITC analysis indicated that the freeze-thaw process caused changes in the interaction properties of GlnK with its ligand ATP. LBA cryoprotectant activity was also evaluated and with both of the techniques it was demonstrated that the compound prevented the damage caused by the freeze-thaw process, thereby maintaining the characteristics of the samples.

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Complexation of Lanthanide(III) Ions with Polyhydroxy Carboxylic Acids in Aqueous Solutions

Giroux

Aury

Henry

et al. 2002

Eur. J. Inorg. Chem.

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The complexation of lanthanum(III), europium(III), dysprosium(III), erbium(III), and lutetium(III) with D‐gluconic acid (LH) was studied by pH potentiometry and NMR. The set of complexes formed in aqueous solutions and their stability constants β were determined, and the results were compared to those previously found for praseodymium(III) (S. Giroux et al., Polyhedron 2000, 19, 1567). A regular evolution of the β values was observed along the lanthanide series, particularly for the neutral complex MLH−2 (where two hydroxy functions were deprotonated) with a selectivity similar to that of EDTA. For the ML2+ complex, where the ligand was coordinated via the carboxylate groups, the analysis of the 13C NMR pseudo‐contact shift showed that the carboxylate group was not bidentate and that there was a participation of the α‐hydroxy group in the coordination. The study of the complexation of PrIII with other hydroxy carboxylic acids: glyceric, threonic, 2‐hydroxybutanoic, and 3‐hydroxybutanoic acids confirms that in the complex MLH−2 the deprotonated hydroxy groups, that intervene in the coordination with the metal ion, are those in α and γ positions relative to the carboxylate group. (© Wiley‐VCH Verlag GmbH, 69451 Weinheim, Germany, 2002)

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Lactobionic and gluconic acid complexes of FeII and FeIII; control of oxidation pathways by an organ transplantation preservant

Cited by 27 publications

References 83 publications

Immobilisation of Cellobiose Dehydrogenase and Laccase on Chitosan Particles as a Multi-Enzymatic System for the Synthesis of Lactobionic Acid

Immobilisation of Cellobiose Dehydrogenase and Laccase on Chitosan Particles as a Multi-Enzymatic System for the Synthesis of Lactobionic Acid

Effects of freezing and the cryoprotectant lactobionic acid in the structure of GlnK protein evaluated by circular dichroism (CD) and isothermal titration calorimetry (ITC)

Complexation of Lanthanide(III) Ions with Polyhydroxy Carboxylic Acids in Aqueous Solutions

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