Lactobionic acid coupled liposomes: an innovative strategy for targeting hepatocellular carcinoma

Abstract

Introduction: Liver cancer or hepatocellular carcinoma (HCC) is a major cause of death worldwide. Targeted delivery of drug to the carcinoma cell can be achieved by conjugation of ligand on the carrier system. Methods and materials: In this study, oxaliplatin-loaded hepatoma-targeted liposome were designed and prepared using galactosylated distearoylphosphatidylethanolamine. The liposomes were prepared by cast film method and coupled with lactobionic acid (LA-LP) using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide as a coupling agent. The coupling was confirmed by infrared spectroscopy. They were further characterized for various parameters such as vesicle shape and surface morphology, size, entrapment efficiency and in vitro release pattern. Results and discussion: The vesicle size of the uncoupled liposome (256 nm) was found to be less than LA-LP (310 nm). The uptake of LA-LP and uncoupled liposomes by BEL7402 HCC cell lines was visualized using fluorescence microscopy that revealed the dependence of liposomal recognition and higher uptake of the LA-LP. Organ distribution studies provided evidence that coupling of lactobionic acid on liposomal surface significantly enhanced the tumor uptake of drug, which is reflected by recovery of higher percentage of drug from tumor as compared to uncoupled liposomes or free drug. Conclusion: These studies suggest them as effective vectors for HCC targeting.

Effect of ligand density and PEG modification on octreotide-targeted liposome via somatostatin receptor in vitro and in vivo

“…As a result, the distribution and efficiency of drug encapsulated by longcirculating immunoliposome are improved. Moreover, the therapy effect also has been improved due to its specifically discernment function for target tissues (Zalipsky, 1995, Mercadal et al, 1999, Torchilin et al, 2001, Susumu et al, 2007, Bansal et al, 2016, Yuan et al, 2016.…”

The Bright Future of Liposome Mediated Drug Delivery

“…The uptake and organ distribution of the LA-LIP revealed that loaded drug prominently accumulated in the tumor. The results showed that liposome were a successful carrier for HCC targeting [15]. In conclusion, liposome is a promising carrier for targeted drug delivery in treating cancers.…”

“…It was reported that the acute or chronic conditions of hepatic failure is fatal to our lives [16]. We knew that liposomes could be utilized for effective targeting delivery by receptor-mediated endocytosis [15]. Higuchi et al [17] showed that Kupffer cell-selective oligonucleotide could be carried through novel Fucose modified liposomes (Fuc-LIP) due to the recognition of fucose receptors on Kupffer cells.…”

Saccharide‐based nanocarriers for targeted therapeutic and diagnostic applications

“…Additionally, galactose functionality has also been introduced into nanocarriers via lactobionic acid modification of nanocarrier‐forming amphiphiles . Chen et al .…”