Lactobionic acid: A high value-added lactose derivative for food and pharmaceutical applications

Gutiérrez, Luis Felipe Higuita; Hamoudi, Safia; Belkacemi, Khaled

doi:10.1016/j.idairyj.2012.05.003

Cited by 110 publications

(73 citation statements)

References 107 publications

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“…First, because of its low price and other supreme properties, lactose is hugely used in the food and pharmaceutical industries. [33][34][35] Second, the synthesis of compound 1 can be achieved rather straight forward, a one-pot reaction by condensation of per-O-acetyl-D-lactose with diosgenin and deprotection, unlike the cases of multistep synthesis routes of other diosgenyl saponins bearing 3 or 4 monosaccharide residues. Concerning the advantages of this compound and the urgent need of new compound to treat OSCC, we carried out the current research.…”

Section: Discussionmentioning

confidence: 99%

Apoptotic Effects of Diosgeninlactoside on Oral Squamous Carcinoma Cells in Vitro and in Vivo

Wang

Sun

et al. 2014

Biological & Pharmaceutical Bulletin

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Diosgenyl saponins possess a variety of biological functions. Herein, we demonstrate a new type of diosgenyl saponin derivatives that inhibit cellular proliferation of oral squamous cell carcinoma (OSCC) cell lines. Thereafter, we analyzed these cells' expression of apoptosis-related proteins. Crucial proteins that participate in apoptosis regulation including caspases 8, 9, and 3, and cleaved Bid were activated and upregulated accompanied by increased concentrations of diosgenyl saponins. Meanwhile, Bcl-2 was downregulated and mitochondrial membrane potential decreased. In our mice model of OSCC, compound 1 showed potent inhibition of solid tumor growth and salient antitumor activity. Diosgenyl β-D-galactopyranosyl-(1→4)-β-Dglucopyranoside might induce OSCC cell line apoptosis through extrinsic and intrinsic pathways, and might provide a mechanistic background for the development of this new type of diosgenylsaponin derivatives into anti-oral cancer agents against OSCC.Key words diosgenyl saponin; oral squamous cell carcinoma (OSCC); apoptosis Oral cancer is one kind of the head and neck tumors, and is the eighth most common occurring cancers in the whole world.1) The factors related to this disease are reported to be tobacco usage, betel quid chewing, excessive alcohol drinking, dietary micronutrient deficiency, and human papilloma virus infection, etc. Its high incidence can be found worldwidely, including nearly all the continents, and caused serious public health threats.2,3) More than 90% of all oral cancers are attributed to oral squamous cell carcinoma (OSCC). OSCC most commonly appears in tongue and the floor of the mouth, whereas it less frequently occurs in other locations such as lip, buccal mucosa, retromolar area, gingiva, soft palate and the back of the tongue and hard palate.4) It is generally accepted that OSCC is caused by mutations of oral keratinocyte: DNA mutations increase hyperplasia of keratinocyte and become pre-malignant or potentially malignant disorders (which are commonly manifested as Leukoplakia or Erythroplakia clinically). Next, they will deteriorate further into the stage of solid cancer or even penetrate across the epithelial basement membrane, and finally metastasize to lymph nodes, bone, brain, liver and other sites.5,6) So it will cause severe threat to the individual life. Also the tendency of its incidence is increasing continuously, around 480000 newly diagnosed cancers per year was reported globally.7) Therefore efficient treatment of OSCC is in urgent demand and is expected to play a key role in combating the overall oral cancers. Current treatment of OSCC has primarily relied on surgery, radiation, and chemotherapy or a combination of these methods. Unfortunately, the symptomatic tend to be diagnosed at an advanced stage, and the 5-year survival rates are still as low as 50% for the past decades. [8][9][10] Since OSCC has a relatively high rate of occult metastases, 11) to the majority of patients with advanced cancer, chemotherapy is an indispensable therapy. However,...

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Section: Discussionmentioning

confidence: 99%

Apoptotic Effects of Diosgeninlactoside on Oral Squamous Carcinoma Cells in Vitro and in Vivo

Wang

Sun

et al. 2014

Biological & Pharmaceutical Bulletin

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“…Moreover, lactose can be chemically transformed through enzymatic and microbial processes to obtain lactobionic acid. Lactobionic acid is a high value product with potential applications as a bioactive molecule in the food and pharmaceutical industries [50]. It is also considered a functional ingredient because of its applications as a prebiotic, acidulant, antioxidant, firming agent and as a chelating agent of metal ions such as Fe [51].…”

Section: Biotechnological Alternatives For the Use Of By-product Wastmentioning

confidence: 99%

Biotechnological Alternatives for the Utilization of Dairy Industry Waste Products

Jesus¹,

Ruth²,

Daniel³

et al. 2015

ABB

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“…[4][5][6] It can also be used as part of drug delivery system because of its binding capacity to asialoglycoprotein (ASGPR) receptors present in hepatic cells. 6,7 An example is its use as a vector of the antitumor drug doxorubicin. 8,9 In the food industry, it has been used as acidulant and stabilizer.…”

Section: Introductionmentioning

confidence: 99%

“…8,9 In the food industry, it has been used as acidulant and stabilizer. 6,7 Some reports show the use of the anaerobic bacterium Zymomonas mobilis to produce lactobionic acid / lactobionate. [10][11][12] This microorganism, presents in its periplasm the glucose-fructose oxidoreductase (GFOR, EC 1.1.99.28)/glucono-lactonase (GL, EC 3.1.1.17) complex.…”

Section: Introductionmentioning

confidence: 99%

Bioproduction and characterization of sodium, potassium, and calcium lactobionates

et al. 2017

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publicado na web em 22/08/2017Lactobionic acid and its salts are substances that have several applications in the pharmaceutical area. These products were obtained by bioconversion through the enzymatic complex glucose-fructose-oxidoreductase (GFOR)/gluconolactonase (GL) present in calcium alginate immobilized cells of Zymomonas mobilis. In the reactions catalyzed by this enzyme system, the medium pH must be controlled at slightly acid values. For this purpose, NaOH, KOH, or Ca(OH) 2 were used, and as a result, the respective salts were formed. The kinetic study on the formation of sodium, potassium and calcium lactobionates was followed by the steps of purification and characterization aiming the potential use of these compounds in the pharmaceutical area. In the assays for the bioproduction of sodium, potassium or calcium lactobionates, yields of 74, 77 and 84% were obtained, respectively. In the salts purification step, purity levels of approximately 95% were achieved. The structural identities of the lactobionate salts were determined by high resolution mass spectrometry, in addition to the 13 C and 1 H NMR analysis. The characterization demonstrates the selectivity of the enzymatic reaction of GFOR/GL of Z. mobilis, in the production of lactobionic acid and its salts.

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Lactobionic acid: A high value-added lactose derivative for food and pharmaceutical applications

Cited by 110 publications

References 107 publications

Apoptotic Effects of Diosgeninlactoside on Oral Squamous Carcinoma Cells <i>in Vitro</i> and <i>in Vivo</i>

Apoptotic Effects of Diosgeninlactoside on Oral Squamous Carcinoma Cells <i>in Vitro</i> and <i>in Vivo</i>

Biotechnological Alternatives for the Utilization of Dairy Industry Waste Products

Bioproduction and characterization of sodium, potassium, and calcium lactobionates

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