Lactobacillus vini: mechanistic response to stress by medium acidification

Mendonça, Allyson Andrade; Silva, Paula Katharina Nogueira da; Calazans, Tiago Luiz Santana; Souza, Rafael Barros de; Pita, Will de Barros; Elsztein, Carolina; Morais, Marcos Antônio de

doi:10.1099/mic.0.000738

Cited by 8 publications

(14 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, glmU was only upregulated under CO 2 intensi cation culture but downregulated under exposure to pH 3 and NaCl concentrations at 7-10%. In comparison with a previous study in Lactobacillus vini, glmU was also downregulated under acid stress conditions (Mendonca et al 2019).…”

Section: Discussioncontrasting

confidence: 67%

Expression of genes involved in exopolysaccharide synthesis in Lactiplantibacillus plantarum VAL6 under environmental stresses

Nguyen

Nguyen-Ho

et al. 2021

Arch Microbiol

View full text Add to dashboard Cite

Environmental factors can alter exopolysaccharide biosynthesis in LAB. To further clarify this potential relationship, the mRNA expression of exopolysaccharide-related genes such as glmU, pgmB1, cps4E, cps4F, cps4J and cps4H genes in Lactiplantibacillus plantarum VAL6 under different conditions including high temperature, acidic or alkaline pH, sodium chloride (NaCl) and carbon dioxide (CO 2 ) intensi cation culture was studied. The transcriptomic data revealed that the exposure of L. plantarum VAL6 to acid at pH 3 increased the expression level of cps4H but decreased the expression levels of pgmB1 and cps4E. Under pH 8 stress, cps4F and cps4E were signi cantly upregulated, whereas pgmB1 was downregulated.Similarly, the expression levels of cps4F, cps4E and cps4J increased sharply under stresses at 42 or 47 o C. In the case of NaCl stress, glmU, pgmB1, cps4J and cps4H were downregulated in exposure to NaCl at 7-10% concentrations while cps4E and cps4F were upregulated at 1 h of 10% NaCl treatment and at 5 h of 4% NaCl treatment. Remarkably, CO 2 intensi cation culture stimulated the expression of all tested genes.In addition, simultaneous changes in expression of cps4E and cps4F under environmental challenges may elicit the possibility of an association between the two genes. These ndings indicated that the expression level of exopolysaccharide-related genes is responsible for changes in the yield and monosaccharide composition of exopolysaccharides under environmental stresses.

show abstract

Section: Discussioncontrasting

confidence: 67%

Expression of genes involved in exopolysaccharide synthesis in Lactiplantibacillus plantarum VAL6 under environmental stresses

Nguyen

Nguyen-Ho

et al. 2021

Arch Microbiol

View full text Add to dashboard Cite

show abstract

“…However, CFE proteomic data did not demonstrate scavenging of citrate, a component of MRS (0.2% triammonium citrate), which was observed to occur during the growth of strain GCRL163 in modified MRS lacking any added carbohydrate source . Alternatively, increased sugar transport may arise from peptidoglycan and exopolysaccharide turnover, given the documented uptake of peptidoglycan breakdown products during normal growth of several Gram-positive species and the importance of this is stationary phase survival, and similar induction of transporters in lactobacilli in response to several stressors. ,, Peptidoglycan turnover during acid stress was recently reported in Lactobacillus vini, leading to increased lysozyme resistance which reflected altered peptidoglycan structure under stress . Furthermore, surface peptidases, including Clp proteases, and ABC transporters for peptides and polar amino acids (notably glutamine transport) were also more highly abundant, which may be linked either with degradation of medium peptides, as observed in strain GCRL163 in the stationary phase once the primary carbohydrate source was depleted, or transport of peptidoglycan degradation products.…”

Section: Discussionmentioning

confidence: 96%

“…87,102,106 Peptidoglycan turnover during acid stress was recently reported in Lactobacillus vini, leading to increased lysozyme resistance which reflected altered peptidoglycan structure under stress. 112 Furthermore, surface peptidases, including Clp proteases, and ABC transporters for peptides and polar amino acids (notably glutamine transport) were also more highly abundant, which may be linked either with degradation of medium peptides, as observed in strain GCRL163 in the stationary phase once the primary carbohydrate source was depleted, 113 or transport of peptidoglycan degradation products. Taken together, these data suggest considerable alteration in the peptidoglycan structure and its decoration by exopolysaccharides for cells cultured at supra-optimal temperatures.…”

Section: ■ Discussionmentioning

confidence: 97%

Prolonged Heat Stress of Lactobacillus paracasei GCRL163 Improves Binding to Human Colorectal Adenocarcinoma HT-29 Cells and Modulates the Relative Abundance of Secreted and Cell Surface-Located Proteins

et al. 2020

View full text Add to dashboard Cite

Lactobacillus casei group bacteria improve cheese ripening and may interact with host intestinal cells as probiotics, where surface proteins play a key role. Three complementary methods [trypsin shaving (TS), LiCl−sucrose (LS) extraction, and extracellular culture fluid precipitation] were used to analyze cell surface proteins of Lactobacillus paracasei GCRL163 by label-free quantitative proteomics after culture to the mid-exponential phase in bioreactors at pH 6.5 and temperatures of 30−45 °C. A total of 416 proteins, including 300 with transmembrane, cell wall anchoring, and secretory motifs and 116 cytoplasmic proteins, were quantified as surface proteins. Although LS caused significantly greater cell lysis as growth temperature increased, higher numbers of extracytoplasmic proteins were exclusively obtained by LS treatment. Together with the increased positive surface charge of cells cultured at supra-optimal temperatures, proteins including cell wall hydrolases Msp1/p75 and Msp2/p40, α-fucosidase AlfB, SecA, and a PspC-domain putative adhesin were upregulated in surface or secreted protein fractions, suggesting that cell adhesion may be altered. Prolonged heat stress (PHS) increased binding of L. paracasei GCRL163 to human colorectal adenocarcinoma HT-29 cells, relative to acid-stressed cells. This study demonstrates that PHS influences cell adhesion and relative abundance of proteins located on the surface, which may impact probiotic functionality, and the detected novel surface proteins likely linked to the cell cycle and envelope stress.

show abstract

“…Whatever the type of metabolism ( Figure 3 ), LAB will always produce lactic acid as a metabolite associated with bacterial growth. However, this weak organic acid can affect biomass yield due to a kind of self-imposed stress [ 123 ]. In its protonated form (at medium pH below 4), lactic acid crosses the cell membrane by simple diffusion [ 124 , 125 ].…”

Section: From the Physiology Of Production To The Processing Of The P...mentioning

confidence: 99%

“…This resistance can be a consequence of genetic modifications that include mutations in the genes encoding enzymes of the peptidoglycan construction, but it can also be induced by modulating the expression of these genes by environmental conditions. In a model of study, the treatment of L. vini cells with sub-MIC concentrations of organic acids (acetic and lactic) or with HCl promoted a change in the constitution of this component to make it resistant to the action of lysozyme, as observed for cells in the stationary growth phase [ 123 ]. The entry into the stationary phase in the case of LAB is characterized by the accumulation of organic acids excreted into the medium [ 222 ].…”

Section: The Probiotic’s Passage Through Git: To the Mouth And Beyondmentioning

confidence: 99%

Journey of the Probiotic Bacteria: Survival of the Fittest

et al. 2022

Self Cite

View full text Add to dashboard Cite

This review aims to bring a more general view of the technological and biological challenges regarding production and use of probiotic bacteria in promoting human health. After a brief description of the current concepts, the challenges for the production at an industrial level are presented from the physiology of the central metabolism to the ability to face the main forms of stress in the industrial process. Once produced, these cells are processed to be commercialized in suspension or dried forms or added to food matrices. At this stage, the maintenance of cell viability and vitality is of paramount for the quality of the product. Powder products requires the development of strategies that ensure the integrity of components and cellular functions that allow complete recovery of cells at the time of consumption. Finally, once consumed, probiotic cells must face a very powerful set of physicochemical mechanisms within the body, which include enzymes, antibacterial molecules and sudden changes in pH. Understanding the action of these agents and the induction of cellular tolerance mechanisms is fundamental for the selection of increasingly efficient strains in order to survive from production to colonization of the intestinal tract and to promote the desired health benefits.

show abstract

Lactobacillus vini: mechanistic response to stress by medium acidification

Cited by 8 publications

References 51 publications

Expression of genes involved in exopolysaccharide synthesis in Lactiplantibacillus plantarum VAL6 under environmental stresses

Expression of genes involved in exopolysaccharide synthesis in Lactiplantibacillus plantarum VAL6 under environmental stresses

Prolonged Heat Stress of Lactobacillus paracasei GCRL163 Improves Binding to Human Colorectal Adenocarcinoma HT-29 Cells and Modulates the Relative Abundance of Secreted and Cell Surface-Located Proteins

Journey of the Probiotic Bacteria: Survival of the Fittest

Contact Info

Product

Resources

About