Lactobacillus strains isolated from oysters improve the production of Crassostrea gigas larvae

Abstract

One of the main problems in laboratories of oyster seed production occurs in the stage of settlement or fixation and metamorphosis of the larvae. The organisms develop drastic morphological and physiological changes at this stage, exposing them to attack by pathogenic microorganisms. Numerous studies indicate that before larval settlement to a substrate, they require bacteria to stimulate and induce the process. In this study, Japanese oyster (Crassostrea gigas) larvae were fed with combinations of microalgae and bacteria previously selected for their probiotic potential; their impact on survival, growth, and settlement was evaluated. Larvae D of 12 days old were provided by a private company and transported to the laboratory under controlled conditions. Probiotic bacteria Lactobacillus plantarum 69Cr, L. fermentum 101Cc, and L. casei 43Cg were reactivated on MRS and applied at a concentration of 1×104 CFU mL-1. Microalgae Isochrysis galbana and Chaetoceros calcitrans were grown in F2 medium and supplied at a concentration of 3×104 cells mL-1. Results showed, in all treatments, a survival range of 39-53%, growth of 300-310 μm, and 34-56.5% settlement. However, the best bacteria combined with I. galbana and C. calcitrans was L. plantarum 69Cr with a 54% survival, 310 μm growth, and 56.5% settlement. It is concluded that the combination of L. plantarum 69Cr with I. galbana and C. calcitrans has the potential to be used in the production of Japanese oyster larvae.

Study of the Impact of Lactiplantibacillus plantarum I on the Health Status of Queen Scallop Aequipecten opercularis

“…In addition, treatment with Lactobacillus spp. isolated from lion's paw scallop has been shown to help maintain the integrity of internal tissues and increase tissue weight [29], which may be reflected in the condition index and meat yield of the scallops in our study.…”

“…Previous studies have reported positive effects of various LAB on the growth performance of both fish and shellfish. In a study conducted by Savin-Amador et al [29], it was observed that Litopenaeus vannamei larvae fed with a diet supplemented with Lactiplantibacillus plantarum 69Cr, Lactiplantibacillus fermentum 101Cc, and Lactiplantibacillus casei, exhibited improved growth performance and survivability. Similarly, the inclusion of the probiotic Lactiplantibacillus acidophilus (FLa) in the diet of Siberian sturgeon (Acipenser baerii) resulted in enhanced growth indices, biochemical content, and blood composition [18].…”

“…Recent studies conducted with Lpb. plantarum demonstrate encouraging outcomes in enhancing the survival [26][27][28][29], growth, and physiological condition of shellfish [30]. While the exact functional role of Lpb.…”

Draft Genome Sequence of Pediococcus pentosaceus Strain PP16CC, Isolated from Oyster Crassostrea corteziensis

“… Crassostrea corteziensis is a potential alternative in oyster farming of Mexican Pacific ( 1 ). However, a main problem has been diseases ( 2 ); therefore, probiotic Pediococcus pentosaceus has been studied to improve oyster survival during seed production ( 3 ).…”