Lactobacillus rhamnosus GG supernatant enhance neonatal resistance to systemic Escherichia coli K1 infection by accelerating development of intestinal defense

Abstract

The objective of this study was to determine whether Lactobacillus rhamnosus GG culture supernatant (LCS) has a preventive effect against gut-derived systemic neonatal Escherichia coli (E. coli) K1 infection. The preventive effects were evaluated in human colonic carcinoma cell line Caco-2 and neonatal rat models. Our in vitro results showed that LCS could block adhesion, invasion and translocation of E. coli K1 to Caco-2 monolayer via up-regulating mucin production and maintaining intestinal integrity. In vivo experiments revealed that pre-treatment with LCS significantly decrease susceptibility of neonatal rats to oral E. coli K1 infection as reflected by reduced bacterial intestinal colonization, translocation, dissemination and systemic infections. Further, we found that LCS treated neonatal rats have higher intestinal expressions of Ki67, MUC2, ZO-1, IgA, mucin and lower barrier permeability than those in untreated rats. These results indicated that LCS could enhance neonatal resistance to systemic E. coli K1 infection via promoting maturation of neonatal intestinal defense. In conclusions, our findings suggested that LCS has a prophylactic effect against systemic E. coli K1 infection in neonates. Future studies aimed at identifying the specific active ingredients in LCS will be helpful in developing effective pharmacological strategies for preventing neonatal E. coli K1 infection.

Lactobacillus casei BL23 Produces Microvesicles Carrying Proteins That Have Been Associated with Its Probiotic Effect

“…It was also shown that p40 of L. rhamnosus GG, stimulates the phosphorylation of the epidermal growth factor receptor in vitro and in vivo in colon epithelial cells and ameliorates intestinal inflammation in mice ( Yan et al, 2011 ; Yoda et al, 2014 ). In accordance with this, He et al (2017) recently showed that the culture supernatant from L. rhamnosus GG enhances resistance to systemic Escherichia coli K1 infection by an increment of intestinal defense in neonates. Although, the authors did not make a direct mention of MVs.…”

Intake of a Mixture of Sake Cake and Rice Malt Increases Mucin Levels and Changes in Intestinal Microbiota in Mice

“…Therefore, the enhancement of mucin production due to SRP intake might increase the population of Lactobacillaceae in the intestine. On the other hand, the application of Lactobacillus rhamnosus GG culture supernatant has been reported to upregulate mucin production and protect from E. coli infection in rats [31]. This result shows another possibility that the intake of SRP increases the intestinal Lactobacillaceae population, which may influence the mucin secretion system and enhance mucin secretion in the intestine.…”

Epithelial-microbial diplomacy: escalating border tensions drive inflammation in inflammatory bowel disease

“…Administration of LGG culture supernatant to neonatal rats was associated with increased IEC proliferation, decreased paracellular barrier permeability, and increased expression of mucin-2, zonula occludens-1 (ZO-1), and IgA, together promoting resistance to neonatal E. coli K1 infection. 97 In addition, LGG enhances wound healing through enterocyte activation of FPR1 and NOX1, similar to A. muciniphila. 98 Direct actions of LGG are performed p40, were found to inhibit proinflammatory cytokine-induced IEC apoptosis.…”