Lactobacillus Probiotics Improve Vaginal Dysbiosis in Asymptomatic Women

Abstract

Vaginal dysbiosis can lead to serious infections in asymptomatic women. Lactobacillus probiotics (LBPs) are being investigated as a promising therapy for reversing vaginal microbiota dysbiosis. This study aimed to investigate whether administering LBPs could improve vaginal dysbiosis and facilitate the colonization of Lactobacillus species in asymptomatic women. 36 asymptomatic women were classified based on the Nugent score as Low-NS (n = 26) and High-NS (n = 10) groups. A combination of Lactobacillus acidophilus CBT LA1, Lactobacillus rhamnosus CBT LR5, and Lactobacillus reuteri CBT LU4 was administered orally for 6 weeks. The study found that among women with a High-NS, 60% showed improved vaginal dysbiosis with a Low-NS after LBP intake, while four retained a High-NS. Among women with a Low-NS, 11.5 % switched to a High-NS. Genera associated with vaginal dysbiosis were positively correlated with the alpha diversity or NS, while a negative correlation was observed between Lactobacillus and the alpha diversity and with the NS. Vaginal dysbiosis in asymptomatic women with an HNS improved after 6 weeks of LBP intake, and qRT-PCR revealed the colonization of Lactobacillus spp. in the vagina. These results suggested that oral administration of this LBP could improve vaginal health in asymptomatic women with an HNS.

Dysbiotic Vaginal Microbiota Induces Preterm Birth Cascade via Pathogenic Molecules in the Vagina

“…Recently, we found asymptomatic BV improved with the treatment of three Lactobacillus spp. combination probiotics [ 31 ].…”

Pilot Study on Next-Generation Sequencing Analysis of Vaginal Microbiota in Clinically Infertile Patients Treated with Probiotics

“…Such probiotic interventions might potentially yield favorable results among infertile individuals, particularly those with RIF. However, despite the promising implications of probiotics’ influence on vaginal health derived from studies [ 24 , 25 , 26 ], their direct impact on enhancing reproductive success remains inconclusive [ 27 , 28 , 29 ]. A randomized controlled trial involving 340 infertile women undergoing frozen embryo transfer and divided into a study group (receiving daily intravaginal lactobacilli tablets for six days) and a control group (no probiotic supplementation) demonstrated comparable biochemical and clinical pregnancy rates between the groups, with a notable reduction in the miscarriage rate within the study group [ 27 ].…”

Simultaneous application of oral and intravaginal probiotics for Helicobacter pylori and its antibiotic-therapy-induced vaginal dysbacteriosis

“…colonization in vagina. Ansari et al 138 Oral Total 89 women diagnosed with bacterial vaginosis and 93 women diagnosed with vulvovaginal candidiasis (VVC) All patients were treated with oral or vaginal probiotic capsules, or placebo capsules for 3 months. BV patients received L. crispatus DSM32717 and L. crispatus DSM32720.…”

“…Their findings revealed that 42.9% of patients in the probiotic group exhibited negative GBS colonization in the vagina, compared to 18.0% in the placebo group, suggesting that oral probiotics could diminish pathogen colonization in the vagina 137 . Moreover, oral administration of L. acidophilus CBT LA1, L. rhamnosus CBT LR5, and L. reuteri CBT LU4 significantly improved vaginal dysbiosis in asymptomatic women and restored the abundance of Lactobacillus spp., resulting in a healthier vaginal microenvironment post-treatment 138 . Additionally, a study investigating probiotic supplementation for bacterial vaginosis and vulvovaginal candidiasis (VVC) demonstrated similar efficacy.…”