Lactobacillus plantarum (VR1) isolated from an Ayurvedic medicine (Kutajarista) ameliorates in vitro cellular damage caused by Aeromonas veronii

Abstract

BackgroundLactobacillus plantarum is considered as a safe and effective probiotic microorganism. Among various sources of isolation, traditionally fermented foods are considered to be rich in Lactobacillus spp., which can be exploited for their probiotic attribute. Antibacterial property of L. plantarum has been demonstrated against various enteric pathogens in both in vitro and in vivo systems. This study was aimed at characterizing L. plantarum isolated from Kutajarista, an ayurvedic fermented biomedicine, and assessing its antagonistic property against a common enteropathogen Aeromonas veronii.ResultsWe report the isolation of L. plantarum (VR1) from Kutajarista, and efficacy of its cell free supernatant (CFS) in amelioration of cytotoxicity caused by Aeromonas veronii. On the part of probiotic attributes, VR1 was tolerant to pH 2, 0.3% bile salts and simulated gastric juice. Additionally, VR1 also exhibited adhesive property to human intestinal HT-29 cell line. Furthermore, CFS of VR1 was antibacterial to enteric pathogens like Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Aeromonas veronii and clinical isolates of P. aeruginosa and E. coli. Detailed study regarding the effect of VR1 CFS on A. veronii cytotoxicity showed a significant decrease in vacuole formation and detrimental cellular changes in Vero cells. On the other hand, A. veronii CFS caused disruption of tight junction proteins ZO-1 and actin in MDCK cell line, which was prevented by pre-incubation with CFS of VR1.ConclusionsThis is the first study to report isolation of L. plantarum (VR1) from Kutajarista and characterisation for its probiotic attributes. Our study demonstrates the antagonistic property of VR1 to A. veronii and effect of VR1 CFS in reduction of cellular damage caused by A. veronii in both Vero and MDCK cell lines.

Pediococcus acidilactici as a potential probiotic to be used in food industry

“…When bile salts were added (small intestine digestion), no significant differences were found ( P = 0.60) between short and long digestions, as slight decreases (about 0.1 log unit reduction for short digestion and about 0.2 log unit reduction for the long digestion) were found for both micro‐organisms. All the strains from the studies mentioned above showed high survival rates after the addition of 0.3% (w/v) of bile salts (Kumar et al ., ; Abbasiliasi et al ., ; Kaboré et al ., ).…”

“…Kumar et al . () studied the strain L. plantarum VR1, which had an increase of 0.3 log unit after 3 h at pH 2.0, also without pepsin.…”

Isolation and characterization of probiotic properties of Lactobacilli isolated from rat fecal microbiota

“…Tolerance of the isolates to bile salts and simulated gastric juice was assayed as described by Kumar et al . . For the bile tolerance test, MRS broth was supplemented with 0.3% (w/v) bile salts (oxgall; Himedia, Mumbai, India).…”

Microbial Community Structure at Different Fermentation Stages of Kutajarista, a Herbal Formulation

“…isolated from alcoholic fermentation of raffia wine has been examined for its immune stimulation activity in mouse model [7]. Previously, we have also reported probiotic attributes of Lactobacillus plantarum isolated from similar sources [15]. At 8 day of fermentation, presence of Streptococcus and Bacillus spp., indicated vigorous stage of fermentation, and reported to have pectinlolytic activity [12,26].…”