Lactobacillus plantarum strains attenuated DSS-induced colitis in mice by modulating the gut microbiota and immune response

Khan, Israr; Wei, Junshu; Li, An‐Ping; Liu, Zhongfan; Yang, Pingrong; Jing, Yaping; Chen, Xinjun; Zhao, Tian; Bai, Yanrui; Zha, Lajia; Li, Chenhui; Che, Tuanjie; Zhang, Chunjiang

doi:10.1007/s10123-022-00243-y

Cited by 16 publications

(10 citation statements)

References 110 publications

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“…The members of the genus Faecalibaculum are known to have antitumor potential in the gut of mice and are capable of producing the metabolites of SCFAs (mainly butyrate) [ 9 , 44 ]. We observed an increase in this genus in mice after four weeks of supplementation with lactic acid bacteria.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have demonstrated significant changes in microbiota after probiotic treatment [ 8 ]. For example, Khan et al showed the beneficial effects of Lactobacillus plantarum on the microbiome of mice, which were demonstrated by an increase in the beneficial bacteria that are associated with the production of short-chain fatty acids and a reduction in pro-inflammatory cytokines [ 9 ]. The addition of L. fermentum prevented hyperlipidemia, inflammation and oxidative stress in the colon and heart tissues of rats that were fed a high-fat diet.…”

Section: Introductionmentioning

confidence: 99%

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Dynamics of Changes in the Gut Microbiota of Healthy Mice Fed with Lactic Acid Bacteria and Bifidobacteria

et al. 2022

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Probiotics are living microorganisms that provide numerous health benefits for their host. Probiotics have various effects on the body; for example, they change gut microbiota, improve the integrity of the epithelial barrier and have anti-inflammatory effects. The use of probiotic supplements that are based on lactic acid bacteria and bifidobacteria is one of the approaches that are used to balance gut microflora. In our study, we evaluated the effects of supplements, which were based on members of the Lactobacillaceae family and bifidobacteria, on the gut microbiome of healthy mice using the 16S rRNA sequencing method. The data that were obtained demonstrated that when mice received the probiotic supplements, statistically significant changes occurred in the composition of the microbiome at the phylum level, which were characterized by an increase in the number of Actinobacteriota, Bacteroidota, Verrucomicrobia and Proteobacteria, all of which have potentially positive effects on health. At the generic level, a decrease in the abundance of members of the Nocardioides, Helicobacter and Mucispirillum genus, which are involved in inflammatory processes, was observed for the group of mice that was fed with lactic acid bacteria. For the group of mice that was fed with bifidobacteria, a decrease was seen in the number of members of the Tyzzerella and Akkermansia genus. The results of our study contribute to the understanding of changes in the gut microbiota of healthy mice under the influence of probiotics. It was shown that probiotics that are based on members of the Lactobacillaceae family have a more positive effect on the gut microbiome than probiotics that are based on bifidobacteria.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Dynamics of Changes in the Gut Microbiota of Healthy Mice Fed with Lactic Acid Bacteria and Bifidobacteria

et al. 2022

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“…For example, antibiotic treatment for up to 4 weeks, especially with vancomycin, increases susceptibility to invasive candidiasis by reducing IL-17A and GM-CSF and promoting non-inflammatory escape of gut bacteria, leading to systemic coinfection and death. 49 This immune response is associated with a decrease in some bacterial families such as Bacteroidales, Deferribacterales, Erysipelotrichales , and Clostridiales , and their short-chain fatty acid-producing species, such as Ruminococcus, Roseburia , and Butyricimonas . 50 These findings were partially confirmed by our study results.…”

Section: Discussionmentioning

confidence: 99%

“…Almost all of them are butyrate-producing bacteria. 49 Studies have demonstrated that short chain fatty acids (SCFAs), have a role in reducing inflammatory immune responses. 51 …”

Section: Discussionmentioning

confidence: 99%

Short- and Long-Term Effects of Different Antibiotics on the Gut Microbiota and Cytokines Level in Mice

Wang¹,

Xiang²,

Gu³

et al. 2022

IDR

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Background Antibiotics are the first line of treatment for infectious diseases. However, their overuse can increase the spread of drug-resistant bacteria. The present study analyzed the impact of different types of antibiotics on the gut microbiome and cytokines level of mice. Methods A total of five groups of 8-week-old male BALB/c mice (n = 35) were treated with piperacillin-tazobactam (TZP), ceftriaxone (CRO), tigecycline (TGC), levofloxacin (LEV) or normal saline (Ctrl), respectively, for up to 4 weeks. Fecal samples were analyzed by bacterial 16S rRNA gene sequencing for bacterial identification. Blood samples were used for the determination of 23 serum cytokines using multiplex immunoassay. Results Exposure to antibiotics was shown to affect the normal weight gain of mice. Significant changes in gut composition caused by TZP, CRO and TGC treatment included the decreased abundance of Bacteroidetes (p < 0.01), Muribaculaceae (p < 0.01) and Lachnospiraceae (p < 0.01), and the increased abundance of Proteobacteria (p < 0.05), Enterobacteriaceae (including Klebsiella and Enterobacter ) (p < 0.01) and Enterococcaceae (including Enterococcus ) (p < 0.01). After 4-week treatment, the TZP, CRO and LEV groups had significantly lower concentrations of several serum cytokines. Correlation analysis of the top 30 bacterial genera and cytokines showed that Enterococcus and Klebsiella were strongly positively correlated with tumor necrosis factor-α (TNF-α), interleukins (IL) IL-12p70 and IL-1β. Desulfovibrio, Candidatus Saccharimonas, norank_f__norank_o__Clostridia_UCG-014, Lactobacillus , and Roseburi a were strongly negatively correlated with these cytokines. Conclusion This study demonstrates the effects of various antibiotics on the intestinal microflora and immune status of mice. Compared with TZP, CRO and TGC, LEV had minimal impact on the gut microbiota. In addition to TGC, long-term TZP, CRO and LEV intervention can lead to a decrease in serum cytokine levels, which may depend on the intestinal microflora, antibiotic used and the duration of treatment.

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“…According to the previous reports, the concentration of LP was selected as 10 9 CFU kg −1 d −1 to ensure that probiotics could achieve sufficient benefits in the body 19 . Based on the recommended dietary allowance (RDA), it is recommended that daily intake of dietary fiber for women is 25 g and for men 38 g 20 .…”

Section: Methodsmentioning

confidence: 99%

Preventive and synbiotic effects of the soluble dietary fiber obtained from Lentinula edodes byproducts and Lactobacillus plantarumLP90 against dextran sulfate sodium‐induced colitis in mice

Xue

Liu

et al. 2022

J Sci Food Agric

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BACKGROUND Soluble dietary fiber (SDF) obtained from Lentinula edodes byproducts has beneficial effects on human intestinal health. This study aimed to examine the combined preventive and ameliorative effects of a kind of synbiotic (SDF with a molecular weight of 1.58 × 102 kDa and Lactobacillus plantarum LP90 (LP) at 1 × 109 CFU kg−1) on dextran sulfate sodium‐induced colitis mice. RESULTS The results demonstrated that synbiotic treatment could alleviate weight loss, decrease the disease activity index level and cause histological amelioration. Synbiotic treatment also promoted the production of goblet cells, increased the expression of tight junction proteins, and adjusted the production of myeloperoxidase, malondialdehyde and superoxide dismutase to repair intestinal epithelial injury. Clinical symptoms were alleviated by maintaining Th17/Treg balance, increasing interleukin 10 and immunoglobulin A levels, reducing interleukin 17a and tumor necrosis factor α production, and promoting mRNA to highly express of Foxp3 and vitamin D receptors. Moreover, synbiotic treatment could upregulate butyric acid production (4.71 ± 0.46 mol g−1 feces, P < 0.05) and diversity of intestinal microbial to maintain intestinal homeostasis. CONCLUSION This study suggested that the combination of LP and SDF as a synbiotic has the potential for use as a nutritional supplement to alleviate colitis. © 2022 Society of Chemical Industry.

show abstract

Lactobacillus plantarum strains attenuated DSS-induced colitis in mice by modulating the gut microbiota and immune response

Cited by 16 publications

References 110 publications

Dynamics of Changes in the Gut Microbiota of Healthy Mice Fed with Lactic Acid Bacteria and Bifidobacteria

Dynamics of Changes in the Gut Microbiota of Healthy Mice Fed with Lactic Acid Bacteria and Bifidobacteria

Short- and Long-Term Effects of Different Antibiotics on the Gut Microbiota and Cytokines Level in Mice

Preventive and synbiotic effects of the soluble dietary fiber obtained from Lentinula edodes byproducts and Lactobacillus plantarumLP90 against dextran sulfate sodium‐induced colitis in mice

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