Lactobacillus plantarum metabolites reduce deoxynivalenol toxicity on jejunal explants of piglets

Maidana, L; Gerez, Juliana Rubira; Hohmann, Miriam S. N.; Verri, Waldiceu A.; Bracarense, Ana Paula Frederico Rodrigues Loureiro

doi:10.1016/j.toxicon.2021.09.023

Cited by 10 publications

(5 citation statements)

References 62 publications

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“…All the values are expressed as the mean ± SD (n = 3). Compared with the control group, *P < 0.05, **P < 0.01; compared with the DON group, # P < 0.05, ## P < 0.01 (Weaver et al 2013;Ma et al 2022;Maidana et al 2021). At the same time, our previous research also found that S. cerevisiae has a certain repair effect on DON-induced IPEC-J2 cell damage, which can increase cell viability and protect cell integrity (Liu et al 2019).…”

Section: Discussionmentioning

confidence: 74%

“…Yeast and lactic acid bacteria are the most widely used probiotics as animal feed additives. Studies have shown that they can alleviate DON-induced porcine intestinal damage (Weaver et al 2013 ; Ma et al 2022 ; Maidana et al 2021 ). At the same time, our previous research also found that S. cerevisiae has a certain repair effect on DON-induced IPEC-J2 cell damage, which can increase cell viability and protect cell integrity (Liu et al 2019 ).…”

Section: Discussionmentioning

confidence: 99%

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Glycyrrhinic acid and probiotics alleviate deoxynivalenol-induced cytotoxicity in intestinal epithelial cells

Chang

Wang

et al. 2023

AMB Expr

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Deoxynivalenol (DON) is one of the most prevalent mycotoxin contaminants, which posing a serious health threat to animals and humans. Previous studies have found that individually supplemented probiotic or glycyrrhinic acid (GA) could degrade DON and alleviate DON-induced cytotoxicity. The present study investigated the effect of combining GA with Saccharomyces cerevisiae (S. cerevisiae) and Enterococcus faecalis (E. faecalis) using orthogonal design on alleviating IPEC-J2 cell damage induced by DON. The results showed that the optimal counts of S. cerevisiae and E. faecalis significantly promoted cell viability. The optimal combination for increasing cell viability was 400 µg/mL GA, 1 × 106 CFU/mL S. cerevisiae and 1 × 106 CFU/mL E. faecalis to make GAP, which not only significantly alleviated the DON toxicity but also achieved the highest degradation rate of DON (34.7%). Moreover, DON exposure significantly increased IL-8, Caspase3 and NF-κB contents, and upregulated the mRNA expressions of Bax, Caspase 3, NF-κB and the protein expressions of Bax, TNF-α and COX-2. However, GAP addition significantly reduced aforementioned genes and proteins. Furthermore, GAP addition significantly increased the mRNA expressions of Claudin-1, Occludin, GLUT2 and ASCT2, and the protein expressions of ZO-1, Claudin-1 and PePT1. It was inferred that the combination of GA, S. cerevisiae, and E. faecalis had the synergistic effect on enhancing cell viability and DON degradation, which could protect cells from DON-induced damage by reducing DON cytotoxicity, alleviating cell apoptosis and inflammation via inhibiting NF-κB signaling pathway, improving intestinal barrier function, and regulating nutrient absorption and transport. These findings suggest that GAP may have potential as a dietary supplement for livestock or humans exposed to DON-contaminated food or feed.

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Section: Discussionmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 99%

Glycyrrhinic acid and probiotics alleviate deoxynivalenol-induced cytotoxicity in intestinal epithelial cells

Chang

Wang

et al. 2023

AMB Expr

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“…The Lactobacillus spp. mixture comprised an equal quantity of three strains: two isolated from broiler chickens ( L. reuteri and L. plantarum , not deposited in GenBank) and one from wheat ( L. plantarum —accession number CP053912) in previous studies [ 52 , 64 , 65 ]. The strains were grown separately in MRS broth and incubated at 37 °C for 24 h under microaerophilic conditions.…”

Section: Methodsmentioning

confidence: 99%

Modulation of Broiler Intestinal Changes Induced by Clostridium perfringens and Deoxynivalenol through Probiotic, Paraprobiotic, and Postbiotic Supplementation

de Souza,

Baptista,

Menck-Costa

et al. 2024

Toxins

Self Cite

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Deoxynivalenol (DON) is a predisposing factor for necrotic enteritis. This study aimed to investigate the effects of a DON and Clostridium perfringens (CP) challenge on the intestinal morphology, morphometry, oxidative stress, and immune response of broilers. Additionally, we evaluated the potential of a Lactobacillus spp. mixture as an approach to mitigate the damage induced by the challenge. One-day-old broiler chickens (n = 252) were divided into seven treatment groups: Control, DON, CP, CP + DON, VL (DON + CP + viable Lactobacillus spp. mixture), HIL (DON + CP + heat-inactivated Lactobacillus spp. mixture), and LCS (DON + CP + Lactobacillus spp. mixture culture supernatant). Macroscopic evaluation of the intestines revealed that the CP + DON group exhibited the highest lesion score, while the VL and HIL groups showed the lowest scores. Microscopically, all Lactobacillus spp. treatments mitigated the morphological changes induced by the challenge. DON increased levels of reactive oxygen species (ROS) in the jejunum, and CP increased ROS levels in the jejunum and ileum. Notably, the Lactobacillus spp. treatments did not improve the antioxidant defense against CP-induced oxidative stress. In summary, a Lactobacillus spp. mixture, whether used as a probiotic, paraprobiotic, or postbiotic, exerted a partially protective effect in mitigating most of the intestinal damage induced by DON and CP challenges.

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“…This finding supports the potential use of this substance at low dosages without causing harm to the intestinal mucosa of piglets; however, the ability to protect the animal against challenge was not explored. Using the same technique, Maidana et al [20] tested the ability of metabolites from Lactobacillus plantarum to reduce DON toxicity in piglet jejunal explants.…”

Section: Methods Using Simple Apparatusmentioning

confidence: 99%

“…Using the same technique, Maidana et al . [ 20 ] tested the ability of metabolites from Lactobacillus plantarum to reduce DON toxicity in piglet jejunal explants.…”

Section: Methods Using Simple Apparatusmentioning

confidence: 99%

A review on the evolution of methods for intestinal in vitro organ culture and its application in veterinary science

Souza

Guedes

2023

Vet World

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Different techniques have been reported in studies of intestinal in vitro organ culture (IVOC). A robust compilation of all available methods is lacking in the literature, making it difficult to choose a method that corresponds to the study's demands. In this review, readers can assess the most available methods, allowing them to evaluate which is more suitable for their purposes and requirements. A simplified view of culturing intestinal explants is presented, highlighting the approachability of IVOC. Relevant findings from diverse veterinarian studies, where explants played a major role, as well as the technique used in each, are described to illustrate its applications. Finally, the strengths and limitations of the innovative intestinal IVOC methods are discussed. This review provides a collection of methods for intestinal explant culture and their possible applications in veterinary research. In this way, it aims to broaden access to IVOC techniques and aid decision-making regarding the best suited for a study's purposes.

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Lactobacillus plantarum metabolites reduce deoxynivalenol toxicity on jejunal explants of piglets

Cited by 10 publications

References 62 publications

Glycyrrhinic acid and probiotics alleviate deoxynivalenol-induced cytotoxicity in intestinal epithelial cells

Glycyrrhinic acid and probiotics alleviate deoxynivalenol-induced cytotoxicity in intestinal epithelial cells

Modulation of Broiler Intestinal Changes Induced by Clostridium perfringens and Deoxynivalenol through Probiotic, Paraprobiotic, and Postbiotic Supplementation

A review on the evolution of methods for intestinal in vitro organ culture and its application in veterinary science

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