Lactobacillus plantarum lipoteichoic acid inhibits biofilm formation of Streptococcus mutans

Ahn, Kyu Jeung; Baik, Jung Eun; Park, Ok Jin; Yun, Cheol Heui; Han, Sang Seop

doi:10.1371/journal.pone.0192694

Cited by 83 publications

(81 citation statements)

References 59 publications

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“…The antibacterial activity of Lactobacillus has been reported previously; for example, the reported Lactobacillus strains showed antibacterial activity against C. difficile [ 45 ], E. coli [ 46 ], Shigella spp. [ 47 ], P. aeruginosa [ 48 ], and S. aureus [ 49 ]. Recently, Wang et al [ 50 ] isolated L. plantarum from Tibetan yaks and reported an inhibitory effect against Staphylococcus aureus and E. coli .…”

Section: Resultsmentioning

confidence: 99%

Probiotic and Antioxidant Potential of Lactobacillus reuteriLR12 and Lactobacillus lactisLL10 Isolated from Pineapple Puree and Quality Analysis of Pineapple-Flavored Goat Milk Yoghurt during Storage

et al. 2020

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In recent years, studies have focused on the therapeutic properties of probiotics to eliminate pathogenic microorganisms associated with various diseases. Lactobacilli are important probiotics groups that have been found to possess many health-promoting activities. This study was carried out to isolate LactobacillusreuteriLR12 and L. lactisLL10 from pineapple puree. The invitro analysis to evaluate probiotic characteristics of the isolated bacteria included survival in bile and acid tolerance. The cell-free supernatant of L. reuteri LR12 was effective against various pathogenic bacteria and fungi compared with L. lactisLL10. These two bacterial strains have strong anti-biofilm activity (100%) against Enterococcus faecalis, Staphylococcus aureus, and Bacillus cereus. The bacterial strains exhibited adhesion properties to HT-29 cells (human colorectal adenocarcinoma). These bacteria showed DPPH- (2,2-diphenyl-1-picryl-hydrazyl-hydrate) free radical scavenging activity, scavenging of hydroxyl radical activity, superoxide radical scavenging activity, and reducing power activity in the range of 72% ± 3%to 89.3% ± 1.7%, 64% ± 2.7%to 66.8% ± 1.5%, 59.8% ± 4.1%to 63.8% ± 2.1%, and 60.4% ± 1.8%to 66.1% ± 3.3%, respectively. Pineapple puree was used as the starter culture with milk for 2 days for yogurt preparation. Pineapple puree increased flavor and showed the physicochemical properties of yogurt. The finding of the sensory evaluation revealed no significant change compared with the control, except the appearance of yogurt. These findings show that Lactobacilli and pineapple puree have potential use in various probiotic preparations for the fermentation industry.

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Section: Resultsmentioning

confidence: 99%

Probiotic and Antioxidant Potential of Lactobacillus reuteriLR12 and Lactobacillus lactisLL10 Isolated from Pineapple Puree and Quality Analysis of Pineapple-Flavored Goat Milk Yoghurt during Storage

et al. 2020

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“…All of the supernatants tested significantly reduced the biomass, and L. paracasei 25.4 was the strain with the highest capacity to affect S. mutans biofilms. Ahn et al [38] also demonstrated that L. plantarum lipoteichoic acid could inhibit the biofilm formation of S. mutans on polystyrene plates stained with CV and on hydroxyapatite discs.…”

Section: Discussionmentioning

confidence: 97%

Inhibitory effect of probiotic Lactobacillus supernatants from the oral cavity on Streptococcus mutans biofilms

Rossoni

Velloso

Barros

et al. 2018

Microbial Pathogenesis

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Probiotics can release bioactive substances that can inhibit the growth and biofilm formation of pathogenic microorganisms such as Streptococcus mutans. In this context, we evaluated whether the supernatants of Lactobacillus strains isolated from caries-free subjects can inhibit S. mutans, one of the most important bacteria for dental caries. First, the supernatants of 22 Lactobacillus strains were screened for antibacterial activity against S. mutans in planktonic cultures. All 22 Lactobacillus strains studied (100%) showed antibacterial activity. Thereafter, the Lactobacillus strains with the greatest reductions in the planktonic S. mutans cultures were tested on biofilms. The L. fermentum 20.4, L. paracasei 11.6, L. paracasei 20.3 and L. paracasei 25.4 strains could significantly reduce the number of S. mutans cells in biofilms formed in hydroxyapatite (p < 0.05). This reduction was also confirmed by scanning electron microscopy analysis and was not caused by the decreased pH value in the medium (p > 0.05). In addition, the supernatants of these probiotic strains could also reduce the total biomass of S. mutans biofilms (p < 0.05). In conclusion, most of the Lactobacillus strains tested have some antibacterial activity against S. mutans. L. fermentum 20.4, L. paracasei 11.6, L. paracasei 20.3 and L. paracasei 25.4 produce bioactive substances that caused a significant reduction in S. mutans biofilms.

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“…The eradication of polymicrobial biofilms in human vagina is one of the strategies that can be used to impede bacterial virulence and prevent the onset of BV [ 201 ]. Lactobacillus plantarum LTA hampered the formation of S. mutans biofilms on hydroxyapatite discs via the attenuation of the sucrose decomposition [ 35 ]. Moreover, L. plantarum LTA significantly inhibited E. faecalis biofilm formation and preformed biofilm on human dentin slices suggesting that LTA can be employed as a preventive and therapeutic measures for E. faecalis infection [ 202 ].…”

Section: Lipoteichoic Acid (Lta)mentioning

confidence: 99%

“…It has been suggested that these beneficial effects are partly associated with the cell surface-active molecules (SAMs) such as peptidoglycan (PG), lipoteichoic acid (LTA), biosurfactants (BS) and exopolysaccharides (EPS) [ 31 , 32 ]. In fact, Lactobacillus SAMs has been proved to antagonise a plethora of bacterial and fungal pathogens such as Candida albicans, Staphylococcus aureus, Streptococcus mutans , Escherichia coli , Pseudomonas aeruginosa , and Salmonella typhimurium [ 33 – 35 ]. Therefore, further understanding in Lactobacillus and its derivatives (i.e.…”

Section: Introductionmentioning

confidence: 99%

Vaginal microbiota and the potential of Lactobacillus derivatives in maintaining vaginal health

2020

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Human vagina is colonised by a diverse array of microorganisms that make up the normal microbiota and mycobiota. Lactobacillus is the most frequently isolated microorganism from the healthy human vagina, this includes Lactobacillus crispatus, Lactobacillus gasseri, Lactobacillus iners, and Lactobacillus jensenii. These vaginal lactobacilli have been touted to prevent invasion of pathogens by keeping their population in check. However, the disruption of vaginal ecosystem contributes to the overgrowth of pathogens which causes complicated vaginal infections such as bacterial vaginosis (BV), sexually transmitted infections (STIs), and vulvovaginal candidiasis (VVC). Predisposing factors such as menses, pregnancy, sexual practice, uncontrolled usage of antibiotics, and vaginal douching can alter the microbial community. Therefore, the composition of vaginal microbiota serves an important role in determining vagina health. Owing to their Generally Recognised as Safe (GRAS) status, lactobacilli have been widely utilised as one of the alternatives besides conventional antimicrobial treatment against vaginal pathogens for the prevention of chronic vaginitis and the restoration of vaginal ecosystem. In addition, the effectiveness of Lactobacillus as prophylaxis has also been well-founded in long-term administration. This review aimed to highlight the beneficial effects of lactobacilli derivatives (i.e. surface-active molecules) with anti-biofilm, antioxidant, pathogen-inhibition, and immunomodulation activities in developing remedies for vaginal infections. We also discuss the current challenges in the implementation of the use of lactobacilli derivatives in promotion of human health. In the current review, we intend to provide insights for the development of lactobacilli derivatives as a complementary or alternative medicine to conventional probiotic therapy in vaginal health.

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Lactobacillus plantarum lipoteichoic acid inhibits biofilm formation of Streptococcus mutans

Cited by 83 publications

References 59 publications

Probiotic and Antioxidant Potential of Lactobacillus reuteriLR12 and Lactobacillus lactisLL10 Isolated from Pineapple Puree and Quality Analysis of Pineapple-Flavored Goat Milk Yoghurt during Storage

Probiotic and Antioxidant Potential of Lactobacillus reuteriLR12 and Lactobacillus lactisLL10 Isolated from Pineapple Puree and Quality Analysis of Pineapple-Flavored Goat Milk Yoghurt during Storage

Inhibitory effect of probiotic Lactobacillus supernatants from the oral cavity on Streptococcus mutans biofilms

Vaginal microbiota and the potential of Lactobacillus derivatives in maintaining vaginal health

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