Lactobacillus plantarum CCFM639 alleviates aluminium toxicity

Abstract

Aluminium (Al) is the most abundant metal in the earth's crust. Al exposure can cause a variety of adverse physiological effects in humans and animals. Our aim was to demonstrate that specific probiotic bacteria can play a special physiologically functional role in protection against Al toxicity in mice. Thirty strains of lactic acid bacteria (LAB) were tested for their aluminium-binding ability, aluminium tolerance, their antioxidative capacity, and their ability to survive the exposure to artificial gastrointestinal (GI) juices. Lactobacillus plantarum CCFM639 was selected for animal experiments because of its excellent performance in vitro. Forty mice were divided into four groups: control, Al only, Al plus CCFM639, and Al plus deferiprone (DFP). CCFM639 was administered at 10(9) CFU once daily for 10 days, followed by a single oral dose of aluminium chloride hexahydrate at 5.14 mg aluminium (LD50) for each mouse. The results showed that CCFM639 treatment led to a significant reduction in the mortality rates with corresponding decrease in intestinal aluminium absorption and in accumulation of aluminium in the tissues and amelioration of hepatic histopathological damage. This probiotic treatment also resulted in alleviation of hepatic, renal, and cerebral oxidative stress. The treatment of L. plantarum CCFM639 has potential as a therapeutic dietary strategy against acute aluminium toxicity.

Potential of Lactobacillus plantarum CCFM639 in Protecting against Aluminum Toxicity Mediated by Intestinal Barrier Function and Oxidative Stress

“…This study demonstrated that chronic Al exposure can cause the disruption of TJ proteins, increase intestinal permeability and induce oxidative stress and inflammatory response. Moreover, we observed that L. plantarum CCFM639 treatment could increase Al excretion [17,18] and protect the intestinal barrier, which acts as the body’s first line of defense against oral Al exposure. Meanwhile, L. plantarum CCFM639 has the ability to alleviate oxidative stress and inflammatory response.…”

“…Our previous study found that a probiotic L. plantarum CCFM639 could reduce Al absorption in the intestines of mice due to its excellent Al-binding ability [17,18]. The decrease of Al absorption in the intestines in turn protects the intestine from Al-induced injures.…”

“…Al exposure causes oxidative stress with a decrease in SOD and CAT activities and a higher level of MDA [17]. It also stimulates production of pro-inflammatory cytokines [2], including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and IL-6.…”

“…The activities of superoxide dismutase (SOD) and catalase (CAT) and the level of malondialdehyde (MDA) were used to evaluate oxidative stress [17]. The level of MDA and activities of SOD and CAT in HT-29 cells and serums of mice were determined by assay kits (Nanjing Jiancheng Bioengineering, Jiangsu, China).…”

“…Our previous studies demonstrated the protection against Al toxicity in vitro and in vivo by the probiotic strain Lactobacillus plantarum CCFM639 was due to its enhanced Al-binding and antioxidative capabilities [17,18]. It significantly inhibits Al absorption in the intestines of mice, thereby decreasing Al accumulation in tissues and alleviating tissue injury caused by this metal.…”