Lactobacillus plantarum and Lactobacillus brevis Alleviate Intestinal Inflammation and Microbial Disorder Induced by ETEC in a Murine Model

Abstract

The purpose of this research is to explore the positive effects of Lactobacillus plantarum and Lactobacillus brevis on the tissue damage and microbial community in mice challenged by Enterotoxigenic Escherichia coli (ETEC). Twenty-four mice were divided into four groups randomly: the CON group, ETEC group, LP-ETEC group and LB-ETEC group. Our results demonstrated that, compared with the ETEC group, the LP-ETEC and LB-ETEC groups experienced less weight loss and morphological damage of the jejunum. We measured proinflammatory factors of colonic tissue and found that L. plantarum and L. brevis inhibited the expression of proinflammatory factors such as IL-β, TNF-α, and IL-6 and promoted that of the tight junction protein such as claudin-1, occludin, and ZO-1. Additionally, L. plantarum and L. brevis altered the impact of ETEC on the intestinal microbial community of mice, significantly increased the abundance of probiotics such as Lactobacillus, and reduced that of pathogenic bacteria such as Proteobacteria, Clostridia, Epsilonproteobacteria, and Helicobacter. Therefore, we believe that L. plantarum and L. brevis can stabilize the intestinal microbiota and inhibit the growth of pathogenic bacteria, thus protecting mice from the gut inflammation induced by ETEC.

Whole Genome Sequencing and CRISPR/Cas9 Gene Editing of Enterotoxigenic Escherichia coli BE311 for Fluorescence Labeling and Enterotoxin Analyses

“…In searching for inhibitors against ETEC as antibiotic alternatives, some infection experimental models are often established based on the ETEC infection [ 51 , 52 , 53 ]. The decreased colonization and proliferation of ETEC in the gastrointestinal tract is a key aim in the control of pathogen infection, making in vivo ETEC tracking particularly important.…”

Agrobacterium sp. ZX09 β-Glucan Attenuates Enterotoxigenic Escherichia coli-Induced Disruption of Intestinal Epithelium in Weaned Pigs

“…The apical brush border structure of intestinal epithelium-specific ZO-1 knockout (KO) mice is disrupted, showing irregular microvillus length and diameter [ 28 ]. The intestinal barrier integrity was impaired upon ETEC challenge [ 29 ], and the serum activity of DAO and the concentration of D-lactic increased following intestinal mucosal injury or epithelial cell shedding [ 30 ]. In our study, their concentrations and the activity increased after ETEC challenge; however, BGL supplementation not only decreased their concentration and activity, but also enhanced the localization of ZO-1 and the concentration and D-xylose in the ETEC-challenged pigs.…”

Combined Microbiome and Metabolome Analysis Reveals a Novel Interplay Between Intestinal Flora and Serum Metabolites in Lung Cancer

“…Correspondingly, the key differential genus in ESLC was Lactobacillus . Lactobacillus itself is a kind of beneficial bacteria, which acts in preventing pathogenic bacteria from invading and colonizing the intestines, enhances the body’s immunity, and has anticancer effects ( Hendler and Zhang, 2018 ; Eslami et al., 2019 ; Han et al., 2021 ). In colorectal cancer, Lactobacillus showed reduced abundance ( Loftus et al., 2021 ).…”