Lactobacillus paragasseri BBM171 Ameliorates Allergic Airway Inflammation Induced by Ovalbumin in Mice via Modulating the Th1/Th2 Balance

Abstract

Supplementation with specific probiotics has been shown to improve allergic airway symptoms. This study aimed to investigate immunomodulatory effects of a potential probiotic strain isolated from breast milk, Lactobacillus paragasseri BBM171 (BBM171), in an ovalbumin (OVA)-induced allergic mouse model. OVA-sensitized and OVA-challenged BALB/c mice were orally administered live or heat-inactivated BBM171 for 48 consecutive days. After the last allergen challenge, serum immunoglobulin (Ig) levels, inflammatory cell levels in the lungs, and cytokine levels in bronchoalveolar lavage fluid (BALF) were assessed. The results showed that oral administration of live or heat-inactivated BBM171 decreased serum levels of total IgE, OVA-specific IgE, and OVA-specific IgG1, while increasing OVA-specific IgG2a and reducing the extent of airway inflammation in OVA-induced allergic mice. In addition, both live and heat-inactivated BBM171 modulated the cytokine profile in BALF to a type 1 T helper (Th1) response. Furthermore, ex vivo experiments using OVA-induced allergic mouse splenocytes showed that both live and heat-inactivated BBM171 could regulate the Th1/Th2 balance, decrease the proinflammatory cytokine interleukin (IL)-17 level, and increase the anti-inflammatory cytokine IL-10 level. Taken together, these results suggest that oral administration of live or heat-inactivated BBM171 improved allergen-induced airway inflammation symptoms by modulating the host immune response toward Th1 dominance.

Lactiplantibacillusplantarum APsulloc331261 (GTB1™) promotes butyrate production to suppress mucin hypersecretion in a murine allergic airway inflammation model

“…GTB1 was administered to mice-induced airway inflammation. PBS was used as the control in this study to eliminate potential media-related effects ( Cheng et al, 2022 ; Bu et al, 2023 ; Duan et al, 2023 ). In our results, oral administration of the strain suppressed type 2 inflammation markers systemically (IgE in serum) and locally (type 2 cytokines and chemokines in BALF and lungs) proving that our target strain modulated the dysregulated immune system in an allergic airway inflammation model.…”

Relationships between Diabetes and the Intestinal Microbial Population

“…The content of butyrate-producing bacteria Lactobacillus (from the Bacillota phylum) is reduced in patients with T2D. These bacteria have also been reported to have anti-allergic effects [34] and to modulate gene expression in a desirable direction tending toward the alleviation of diabetes [35].…”

Heat-killed Lactobacillus paracasei JY56 can relieve ovalbumin-induced food allergy via regulating immuno-inflammatory responses and gut microbiota

“…Interestingly, the immunoregulatory function of inactivated probiotics has gradually attracted widespread attention. Cheng, et al [7] have demonstrated that HK-Lactobacillus paragasseri BBM171 can reduce airway inflammation in OVA-induced allergic mice by modulating serum immunoglobulin (Ig) levels, regulating the Th1/Th2 balance, decreasing levels of the pro-inflammatory cytokine interleukin (IL)-17, and increasing levels of the anti-inflammatory cytokine IL-10. And live L. paragasseri BBM171 and HK-L. paragasseri BBM171 had comparable alleviating effects.…”