Lactobacillus floricola sp. nov., lactic acid bacteria isolated from mountain flowers

Abstract

Five strains (Ryu1-2 T , Gon2-9, Ryu4-3, Nog8-1 and Aza1-1) of lactic acid bacteria were isolated from flowers in mountainous areas in Japan, Oze National Park, Iizuna mountain and the Nikko area. The five isolates were found to share almost identical (99.6-100 % similar) 16S rRNA gene sequences and were therefore deemed to belong to the same species. These isolates exhibited low levels of 16S rRNA gene sequence similarity to known lactic acid bacteria; the closest recognized relatives to strain Ryu1-2 T were the type strains of Lactobacillus hilgardii (92.8 % similarity), Lactobacillus kefiri (92.7 %), Lactobacillus composti (92.6 %) and Lactobacillus buchneri (92.4 %). Comparative analyses of rpoA and pheS gene sequences demonstrated that the novel isolates did not show significant relationships to other Lactobacillus species. The strains were Gram-stain-positive, catalase-negative and homofermentative. The isolates utilized a narrow range of carbohydrates as sources of carbon and energy, including glucose and fructose. On the basis of phenotypic characteristics and phylogenetic data, these isolates represent a novel species of the genus Lactobacillus, for which the name Lactobacillus floricola sp. nov. is proposed. The type strain is Ryu1-2 T (5NRIC 0774 T 5JCM 16512 T 5DSM 23037 T ).Lactobacillus strains have been isolated from several plant sources such as fruit, grass, leaves, tree sap, flowers, fermented vegetables and fermented beverages such as wine, malt whisky, shochu and beer (summarized by

Lactobacillus ozensis sp. nov., isolated from mountain flowers

“…We recently reported the isolation of a novel LAB, Lactobacillus floricola, from a mountainous area (Kawasaki et al, 2011). L. floricola showed quite low 16S rRNA gene sequence similarity to known LAB, and also showed a narrow range of carbohydrate utilization, using only glucose and fructose.…”

“…glandulosa, a chrysanthemum (Japanese common name, oze-mizugiku), that was collected in August 2008. A large number of colonies (10 4 -10 8 colonies per flower) were obtained, and the 16S rRNA gene sequences of randomly selected colonies suggested that these isolates represent the most abundant species in each flower (data not shown).Morphological, physiological and biochemical characteristics were determined according to Okada et al (1992), Holdeman et al (1977) and Gerhardt et al (1981), as described previously (Kawasaki et al, 2011). Lactobacillus buchneri NRIC 1040 T , L. kefiri NRIC 1693 T , L. kunkeei DSM 12361 T , L. floricola NRIC 0774 T and L. fructivorans NRIC 0224 T were used as reference strains in this study.…”

“…Morphological, physiological and biochemical characteristics were determined according to Okada et al (1992), Holdeman et al (1977) and Gerhardt et al (1981), as described previously (Kawasaki et al, 2011). Lactobacillus buchneri NRIC 1040 T , L. kefiri NRIC 1693 T , L. kunkeei DSM 12361 T , L. floricola NRIC 0774 T and L. fructivorans NRIC 0224 T were used as reference strains in this study.…”

“…Morphological, physiological and biochemical characteristics of the isolates were determined using MRS broth as the basal medium as described previously (Kawasaki et al, 2011). The detailed characteristics of the isolates are described in the species description.…”

Draft Genome Sequences of Two Lactobacillus Strains, L. farraginis JCM 14108 T and L. composti JCM 14202 T , Isolated from Compost of Distilled Shōchū Residue

“…The 16S rRNA gene sequence analyses indicate that L. farraginis is closely related to Lactobacillus hilgardii , isolated from wine, and Lactobacillus buchneri , isolated from silages (1). The 16S rRNA gene sequence identities of L. composti to known Lactobacillus species are <93% (2, 3). …”