Lactobacillus fermentum UCO-979C strongly inhibited Helicobacter pylori SS1 in Meriones unguiculatus

Abstract

Searching for bacterial probiotics active upon Helicobacter pylori continue to be an important clinical challenge because of the increased prevalence of this highly priority pathogen in humans. In this work, we assess the in vivo anti-H. pylori SS1 (cagA+/vacAs2m2+) properties of a previously isolated human gastric probiotic strain Lactobacillus fermentum UCO-979C by using a Meriones unguiculatus (Mongolian gerbil) model. Animals were administered with a saline suspension of L. fermentum UCO-979C or H. pylori SS1 as negative and positive control for H. pylori colonisation controls, prior to assayed the challenge group that was administered with these two species per animal for detecting protective activity of the probiotic strain against colonisation. The results showed that L. fermentum UCO-979C strongly inhibited the colonisation of H. pylori decreasing up to 87% of the colonisation in the antrum by the pathogen, suggesting that this probiotic strain has a strong probiotic activity against H. pylori in the most valuable animal model for in vivo assays nowadays.

Consumption of a Gelatin Supplemented with the Probiotic Strain Limosilactobacillus fermentum UCO-979C Prevents Helicobacter pylori Infection in a Young Adult Population Achieved

“…The long-term administration of probiotics might have positive results in H. pylori infection, particularly in reducing the risk to develop diseases caused by the high levels of gastric inflammation [ 65 ]. Moreover, the results of the present work confirm previous results reported for this probiotic strain [ 21 , 22 , 26 , 50 , 66 , 67 ], which may even be considered as a potential immunobiotic capable of reducing the adhesion of H. pylori to the gastric mucosa and the inflammatory damage caused by this pathogen, modulating the immune responses at the intestinal mucosa and systemic levels [ 22 ].…”

The Exopolysaccharide of Lactobacillus fermentum UCO-979C Is Partially Involved in Its Immunomodulatory Effect and Its Ability to Improve the Resistance against Helicobacter pylori Infection

“…This probiotic strain was selected between other of lactic acid bacteria isolated from human gastric tissue because of its remarkable anti-H. pylori properties [41]. The UCO-979C strain strongly inhibited the adhesion, growth and urease activity of H. pylori in AGS cells and Mongolian gerbils [16,42]. Moreover, L. fermentum UCO-979C was able to beneficially modulate the cytokine response of AGS cells and THP-1 macrophages after H. pylori challenge [17].…”

Potential Role of Biofilm Formation in the Development of Digestive Tract Cancer With Special Reference to Helicobacter pylori Infection

“…These results were consistent with a gastric microbiome study in humans showing increased abundance of Lactobacillus in Hp -associated intestinal metaplasia and intestinal type of gastric cancer, compared with non-atrophic gastritis (Aviles-Jimenez et al, 2014) as well as the increased gastric Lactobacillus population in INS-GAS mouse model co-infected with Hp and limited commensals ( Bacteroides, Clostridium , and Lactobacillus ) that developed GIN (Lertpiriyapong et al, 2014). However, others have reported a probiotic Lactobacillus strain that inhibited the colonization of Hp in a Mongolian gerbil model (Merino et al, 2018). More relevant to biofilm-associated Hp , Khosravi et al (2014) reported that Streptococcus mitis induced Hp conversion to coccoid cells in co-culture studies and their proteomic analysis revealed a metabolic crosstalk between these two bacteria, suggesting a probable impact on Hp -associated carcinogenesis (Khosravi et al, 2016; Krzyżek, 2017).…”