Lactobacillus fermentum 90 TC-4 taxonomic status confirmation using whole genome sequencing and MALDI TOF mass spectrum

Belova, Irina V.; Точилина, А. Г.; Соловьева, И. В.; Ефимов, Е. И.; Gorlova, I. S.; Ivanova, T. P.; Жирнов, В А

doi:10.1134/s1022795416090039

Cited by 5 publications

(2 citation statements)

References 13 publications

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“…It is naturally present in raw milk, dairy products, and many traditional fermented foods and beverages [ 3 , 4 ]. Some probiotic isolates, such as L. fermentum RC-14 and L. fermentum 90 TC-4, are already widely used and produced on an industrial scale [ 5 , 6 ]. The species is listed in the qualified presumption of safety (QPS) published by the European Food Safety Authority (EFSA) [ 7 ], and along with other lactic acid bacteria (LAB), it has been generally recognized as safe (GRAS status) by the U.S. Food and Drug Administration.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Erythromycin and Tetracycline Resistance in Lactobacillus fermentum Strains

Anisimova

Yarullina

2018

International Journal of Microbiology

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Lactobacillus fermentum colonizing gastrointestinal and urogenital tracts of humans and animals is widely used in manufacturing of fermented products and as probiotics. These bacteria may function as vehicles of antibiotic resistance genes, which can be transferred to pathogenic bacteria. Therefore, monitoring and control of transmissible antibiotic resistance determinants in these microorganisms is necessary to approve their safety status. The aim of this study was to characterize erythromycin and tetracycline resistance of L. fermentum isolates and to estimate the potential transfer of resistance genes from lactobacilli to the other Gram-positive and Gram-negative bacteria. Among six L. fermentum strains isolated from human feces and commercial dairy products, five strains demonstrated phenotypic resistance to tetracycline. PCR screening for antibiotic resistance determinants revealed plasmid-located tetracycline resistance genes tet(K) and tet(M) in all strains and erythromycin resistance genes erm(B) in the chromosome of L. fermentum 5-1 and erm(C) in the plasmid of L. fermentum 3-4. All tested lactobacilli lacked conjugative transposon Tn916 and were not able to transfer tetracycline resistance genes to Staphylococcus aureus, Staphylococcus epidermidis, Listeria monocytogenes, Acinetobacter baumannii, Citrobacter freundii, and Escherichia coli by filter mating. Staphylococcus haemolyticus did not accept erythromycin resistance genes from corresponding Lactobacillus strains. Thus, in the present study, L. fermentum was not implicated in the spread of erythromycin and tetracycline resistance, but still these strains pose the threat to the environment and human health because they harbored erythromycin and tetracycline resistance genes in their plasmids and therefore should not be used in foods and probiotics.

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Section: Introductionmentioning

confidence: 99%

Characterization of Erythromycin and Tetracycline Resistance in Lactobacillus fermentum Strains

Anisimova

Yarullina

2018

International Journal of Microbiology

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“…Lyophilized strains of L. plantarum 8 RA 3, L. fermentum 90 TC 4, and L. fermentum 39 were grown according to the method described previously [ 12 ], using liquid and agarized MRS nutrient medium (Syntex, Russia). Recovery and inoculation of strains of B. bifidum 1, B. bifidum 791, and B. longum 379 were carried out according to a similar scheme using the HMM liquid medium (hydrolyzate-milk medium, Syntex, Russia) and Bifidobacterium agar medium (HiMedia, India).…”

Section: Methodsmentioning

confidence: 99%

Genome Features and In Vitro Activity against Influenza A and SARS-CoV-2 Viruses of Six Probiotic Strains

Соловьева

Ilyicheva

Marchenko

et al. 2021

BioMed Research International

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Purpose. The aim of this work was to analyze the complete genome of probiotic bacteria Lactobacillus plantarum 8 RA 3, Lactobacillus fermentum 90 TC-4, Lactobacillus fermentum 39, Bifidobacterium bifidum 791, Bifidobacterium bifidum 1, and Bifidobacterium longum 379 and to test their activity against influenza A and SARS-CoV-2 viruses. Methods. To confirm the taxonomic affiliation of the bacterial strains, MALDI TOF mass spectrometry and biochemical test systems were used. Whole genome sequencing was performed on the Illumina Inc. MiSeq platform. To determine the antiviral activity, A/Lipetsk/1V/2018 (H1N1 pdm09) (EPI_ISL_332798) and A/common gull/Saratov/1676/2018 (H5N6) (EPI_ISL_336925) influenza viruses and SARS-CoV-2 virus strain Australia/VIC01/2020 (GenBank: MT007544.1) were used. Results. All studied probiotic bacteria are nonpathogenic for humans and do not contain the determinants of transmission-type antibiotic resistance and integrated plasmids. Resistance to antibiotics of different classes is explained by the presence of molecular efflux pumps of the MatE and MFS families. Cultures of L. fermentum 90 TC 4, L. plantarum 8 RA 3, and B. bifidum 791 showed a pronounced activity against influenza A viruses in MDCK cells. Activity against the SARS-CoV-2 virus was demonstrated only by the L. fermentum 90 TC 4 strain in VERO cells. Conclusions. The studied probiotic bacteria are safe, have antiviral activity, and are of great importance for the prevention of diseases caused by respiratory viruses that can also infect the human intestine.

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Cold Temperature Regulation of Zoospore Release in Phytophthora sojae: The Genes That Differentially Expressed by Cold Temperature

Xuehui

Rui

et al. 2018

Russ J Genet

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Lactobacillus fermentum 90 TC-4 taxonomic status confirmation using whole genome sequencing and MALDI TOF mass spectrum

Cited by 5 publications

References 13 publications

Characterization of Erythromycin and Tetracycline Resistance in Lactobacillus fermentum Strains

Characterization of Erythromycin and Tetracycline Resistance in Lactobacillus fermentum Strains

Genome Features and In Vitro Activity against Influenza A and SARS-CoV-2 Viruses of Six Probiotic Strains

Cold Temperature Regulation of Zoospore Release in Phytophthora sojae: The Genes That Differentially Expressed by Cold Temperature

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