Lactobacillus equi sp. nov., a predominant intestinal Lactobacillus species of the horse isolated from faeces of healthy horses.

Morotomi, Masami; Yuki, Norikatsu; Kado, Yukiko; Kushiro, Akira; Shimazaki, Tomoko; Watanabe, Koichi; Yuyama, Teruhiko

doi:10.1099/00207713-52-1-211

Cited by 49 publications

(35 citation statements)

References 9 publications

(6 reference statements)

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“…These findings would indicate that the Lactobacillus group community in horse feces is not similar to those in any other animal feces. Lactobacillus agilis, L. crispatus, L. mucosae, and L. reuteri have been isolated from feces and gastrointestinal contents of horses by culturing techniques (Bailey et al, 2003;Morotomi et al, 2002), but they were not detected by PCR-DGGE in the present study. This discrepancy may be due to a difference of techniques used or individual differences in horses.…”

Section: Discussioncontrasting

confidence: 69%

“…1 and Table 2. Of the five species detected of the Lactobacillus group, to our knowledge, L. equi has been isolated only from horse feces (Morotomi et al, 2002), a phylogenetic relative of L. salivarius has been detected only on horse manure (Simpson et al, 2004;accession no. AY212750), and a phylogenetic relative of L. gastricus has never been isolated from or detected on any other samples.…”

Section: Discussionmentioning

confidence: 89%

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Molecular profiling of Lactobacillus, Streptococcus, and Bifidobacterium species in feces of active racehorses

Endo

Okada

2007

J. Gen. Appl. Microbiol.

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Section: Discussioncontrasting

confidence: 69%

Section: Discussionmentioning

confidence: 89%

Molecular profiling of Lactobacillus, Streptococcus, and Bifidobacterium species in feces of active racehorses

Endo

Okada

2007

J. Gen. Appl. Microbiol.

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“…Of these, only two bands were found to be common in all five horses. According to BLAST of near full-length 16S rRNA gene sequences (41455 nt) the clones that formed these bands had the highest identity with Lactobacillus equi (AB048833: 99.4%) and Lactobacillus hayakitensis (AB267406: 99.7%), which are considered to be commensals of the equine gastrointestinal tract (Morotomi et al, 2002;Morita et al, 2007). Intensification of the lactobacilli bands was not as marked as for other species, such as the EHSS and E. coli 3, and appeared to be secondary to EHSS proliferation (Figure 1).…”

Section: Anaerovibriomentioning

confidence: 99%

Microbial ecology of the equine hindgut during oligofructose-induced laminitis

et al. 2008

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Alimentary carbohydrate overload is a significant cause of laminitis in horses and is correlated with drastic shifts in the composition of hindgut microbiota. Equine hindgut streptococcal species (EHSS), predominantly Streptococcus lutetiensis, have been shown to be the most common microorganisms culturable from the equine caecum prior to the onset of laminitis. However, the inherent biases of culture-based methods are estimated to preclude up to 70% of the normal caecal microbiota. The objective of this study was to evaluate bacterial population shifts occurring in the equine caecum throughout the course of oligofructose-induced laminitis using several cultureindependent techniques and to correlate these with caecal lactate, volatile fatty acid and degrees of polymerization 3-7 fructo-oligosaccharide concentrations. Our data conclusively show that of the total microbiota present in the equine hindgut, the EHSS S. lutetiensis is the predominant microorganism that proliferates prior to the onset of laminitis, utilizing oligofructose to produce large quantities of lactate. Population shifts in lactobacilli and Escherichia coli subpopulations occur secondarily to the EHSS population shifts, thus confirming that lactobacilli and coliforms have no role in laminitis. A large, curved, Gram-negative rod previously observed during the early phases of laminitis induction was most closely related to the Anaerovibrio genus and most likely represents a new, yet to be cultured, genus and species. Correlation of fluorescence in situ hybridization and quantitative real-time PCR results provide evidence supporting the hypothesis that laminitis is associated with the death en masse and rapid cell lysis of EHSS. If EHSS are lysed, liberated cellular components may initiate laminitis.

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“…To differentiate them, isolates Mizu2-1 T , Gon2-7, Koba6-1, Koyu2-2 and Miya2-2 were analysed by randomly amplified polymorphic DNA-PCR (RAPD-PCR) according to the method of On: Fri, 11 May 2018 18:37:06 Akopyanz et al (1992) using two primers (primer-1, 59-GAGGACAAAG; primer-2, 59-GGCATCGGTT) (Morotomi et al, 2002). RAPD-PCR demonstrated genotypic differences (Fig.…”

mentioning

confidence: 99%

Lactobacillus ozensis sp. nov., isolated from mountain flowers

Kawasaki

Kurosawa

Miyazaki

et al. 2011

International Journal of Systematic and Evolutionary Microbiology

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Five strains (Mizu2-1 T , Gon2-7, Koba6-1, Koyu2-2 and Miya2-2) of lactic acid bacteria (LAB) were isolated from flowers in Oze National Park, Japan, using anaerobic cultivation. The five isolates were found to share identical 16S rRNA gene sequences. The isolates exhibited low levels of 16S rRNA gene sequence similarity to known LAB; the closest recognized relatives of strain Mizu2-1 T were the type strains of Lactobacillus kunkeei (94.9 %), Lactobacillus kefiri (94.1 %) and Lactobacillus buchneri (93.9 %). Comparative analyses of rpoA and pheS gene sequences demonstrated that the novel isolates did not show significant relationships to other Lactobacillus species. The strains were Gram-stain-positive, catalase-negative and heterofermentative. Anaerobic growth was better than aerobic growth. The isolates utilized a narrow range of carbohydrates as sources of carbon and energy, including glucose and fructose. On the basis of phenotypic characteristics and phylogenetic data, the isolates represent a novel species of the genus Lactobacillus, for which the name Lactobacillus ozensis sp. nov. is proposed. The type strain is Mizu2-1 T (5JCM 17196 T 5DSM 23829 T ).During our studies on the distribution of anaerobes in flowers, we have isolated several species of lactic acid bacteria (LAB) from mountain flowers in Japan. We recently reported the isolation of a novel LAB, Lactobacillus floricola, from a mountainous area (Kawasaki et al., 2011). L. floricola showed quite low 16S rRNA gene sequence similarity to known LAB, and also showed a narrow range of carbohydrate utilization, using only glucose and fructose. Here, we report the isolation of further isolates that exhibit low 16S rRNA gene sequence similarity to known LAB and show a narrow range of carbohydrate utilization. During the course of our investigations of anaerobes in flowers from several mountainous areas in Japan since 2006, the novel species described in this study has been isolated only from the area of Oze National Park.Flowers were collected from Oze National Park in [2008][2009]. Flower samples were collected using autoclaved forceps and transferred immediately to sterile tubes. Bacteria were cultivated on MRS agar (Difco) containing 5.0 g calcium carbonate and 15 g agar l 21 at 20-30 u C under anaerobic conditions. After isolation, strains were maintained in MRS broth. The origins of the isolates are shown in Supplementary Fig. S1. The proposed type strain Mizu2-1 T was isolated from a flower of Inula ciliaris var. glandulosa, a chrysanthemum (Japanese common name, oze-mizugiku), that was collected in August 2008. A large number of colonies (10 4 -10 8 colonies per flower) were obtained, and the 16S rRNA gene sequences of randomly selected colonies suggested that these isolates represent the most abundant species in each flower (data not shown).Morphological, physiological and biochemical characteristics were determined according to Okada et al. (1992), Holdeman et al. (1977) and Gerhardt et al. (1981), as described previously (Kawasaki et al., 2011...

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Lactobacillus equi sp. nov., a predominant intestinal Lactobacillus species of the horse isolated from faeces of healthy horses.

Cited by 49 publications

References 9 publications

Molecular profiling of Lactobacillus, Streptococcus, and Bifidobacterium species in feces of active racehorses

Molecular profiling of Lactobacillus, Streptococcus, and Bifidobacterium species in feces of active racehorses

Microbial ecology of the equine hindgut during oligofructose-induced laminitis

Lactobacillus ozensis sp. nov., isolated from mountain flowers

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