Lactobacillus delbrueckii subsp. bulgaricus strain TCI904 reduces body weight gain, modulates immune response, improves metabolism and anxiety in high fat diet-induced obese mice

Abstract

The multiple probiotic characteristics of strain TCI904 isolated in this study from natural fermented milk were investigated using a mouse model. TCI904 was identified as Lactobacillus delbrueckii subsp. bulgaricu (LDB), a well-known lactic acid starter bacterium found in yogurt. TCI904 exhibited an outstanding pancreatic lipase inhibition activity among several strains of lactic acid bacteria in vitro. Its in vivo effects were further studied. In a comparison of mice fed a high-fat diet (HFD) and those fed a HFD combined with TCI904 for 9 weeks, differences were observed in various aspects of health, and the adverse effects of a HFD were prevented in the latter group. TCI904 effectively prevented fat and body weight accumulation without reducing food intake; it also modulated innate immunity and increased the level of IgA in feces, reversing the increased blood sugar and insulin levels and attenuated the hyperlipidemia caused by a HFD. Based on biochemical test data, compared with the HFD group, a HFD combined with TCI904 induced significant lowering of insulin resistance indicator, homeostasis model assessment-insulin resistance (HOMA-IR) and atherogenic indices of plasma (AIP), the atherogenic coefficient (AC) and cardiac risk ratio (CRR) and increased the cardioprotective index (CPI). In addition, the administration of TCI904 alleviated mood disorders caused by a HFD. Taking the recommended human dose of TCI904 did not affect the liver or kidney function, indicating that TCI904 has sufficient in vivo safety. Taken together, the results of the present study contributed towards validation of the probiotic benefits of lactic acid starter microflora. Orally taken TCI904 exhibited positive immune- and metabolic-modulating, and anxiolytic properties, especially in HFD-induced obesity.

The Efficacy of Lactobacillus delbrueckii ssp. bulgaricus Supplementation in Managing Body Weight and Blood Lipids of People with Overweight: A Randomized Pilot Trial

“…Over the past few years, probiotics have gained recognition as a promising avenue for the development of therapeutic and preventative solutions for addressing metabolic syndrome. Several species of Lactobacillus, including L. casei, L. fermentum, L. acidophilus, L. rhamnosus, L. paracasei, and L. bulgaricus, have been documented to possess various probiotic functions, such as reducing blood lipid levels, safeguarding cardiovascular health, and mitigating the symptoms associated with obesity, in diet-induced obesity models [16,[19][20][21][22]. The present study is the first to investigate the effects of L. bulgaricus on obesity control in human subjects.…”

“…bulgaricus (L. bulgaricus) is one of the most common lactobacilli starters used in the manufacture of a large variety of fermented milk products. L. bulgaricus is reported to exhibit an outstanding inhibitory effect on pancreatic lipase activity in vitro and effectively managed the fat and weight accumulation and reversed the increased blood lipid, sugar, and insulin levels caused by a high-fat diet in mice [16]. Our hypothesis posits that L. bulgaricus can contribute to the reduction in body weight and alleviate health risk factors associated with obesity in overweight individuals.…”