Lactobacillus delbrueckii subsp. bulgaricus KLDS 1.0207 Exerts Antimicrobial and Cytotoxic Effects in vitro and Improves Blood Biochemical Parameters in vivo Against Notable Foodborne Pathogens

Abstract

Globally, foodborne diseases (FBDs) result in millions of sicknesses and deaths annually. Cumulative evidence suggests that the use of probiotic lactic acid bacteria (LAB) strains could be a viable alternative in inhibiting the activities of foodborne pathogens. This study aims to evaluate the in vitro antimicrobial, cytotoxic, and tolerance levels of Lactobacillus bulgaricus KLDS 1.0207 against two notable foodborne pathogens-Escherichia coli ATCC25922 and Staphylococcus aureus ATCC25923. Afterward, a 48 BALB/c mice-trial was used to assess its ameliorative effects on weight and serum biochemical parameters. Results showed that the cell-free supernatant (CFS) of this strain significantly inhibited both pathogens, but these effects were abolished at pH 6.5 and 7.0 (P < 0.05). Also, 6.96 ± 0.02 log CFU mL −1 of L. bulgaricus KLDS 1.0207 was still viable after three hours in simulated gastric juice and at pH 3.0, indicating that this strain was a potential probiotic candidate. Also, inflammatory activities in RAW264.7 cells were significantly inhibited using 10 9 CFU mL −1 of L. bulgaricus KLDS 1.0207 cells (P < 0.05). Significant weight losses were also prevented in the T LBSA (from 19.42 ± 1.04 to 19.55 ± 0.55 g) and T LBEC (from 22.86 ± 0.90 to 14.77 ± 9.86 g) groups compared to their respective model groups (T SA-from 21.65 ± 1.80 to 20.14 ± 1.84, and T EC-from 21.45 ± 0.82 to 14.45 ± 9.70 g). Besides, there was a slight weight gain in the S. aureus prevention group (T LBSA) compared to the model group (T SA). Serum biochemical analyses revealed that the total cholesterol (TC), triglycerides (TG), low-density lipoprotein (LDL), and some mineral levels were markedly increased by S. aureus and E. coli administrations but were reversed to normalcy in both prevention groups (T LBSA and T LBEC). Interestingly, high-density lipoprotein

Bioprospecting for Novel Probiotic Strains from Human Milk and Infants: Molecular, Biochemical, and Ultrastructural Evidence

“…These isolates showed the nearest similarity to E. faecium and L. delbrueckii strains. The previous investigation by Evivie et al [ 40 ] confirmed the medicinal usage of L. delbrueckii isolates as a probiotic against foodborne pathogens. Moreover, recent studies have evidenced the role of E. faecium as a promising probiotic candidate for both human and animal use [ 41 , 42 , 43 , 44 ].…”

Genomic Characterization of Lactobacillus delbrueckii Strains with Probiotics Properties

“…The antibacterial effect associated with these L. delbrueckii -producing compounds against some pathogens, such as Salmonella sp. , Enterococcus faecalis , Escherichia coli , Gardnerella vaginalis , Listeria monocytogens , Pseudomonas aeruginosa , has been previously reported ( Evivie et al, 2020 ; De Jesus LCL. et al, 2021 ; Qiu et al, 2021 ).…”

Cranberry Arabino-Xyloglucan and Pectic Oligosaccharides Induce Lactobacillus Growth and Short-Chain Fatty Acid Production

“…Similarly, several studies have investigated the probiotic potential of L. plantarum and L. paraplantarum [ 66 ]; L. delbreuckii subsp. bulgaricus [ 67 , 68 ]; L. buchneri [ 69 ] and L. reuteri [ 70 ]. B. breve 2141 was also shown to grow on cranberry xyloglucan, and several strains of this species have been investigated for treatment of targeted pediatric diseases, including diarrhea, infant colic, celiac disease, obesity, allergies, and neurological disorders [ 71 ].…”