Lactobacillus delbrueckii CRL 581 Differentially Modulates TLR3-Triggered Antiviral Innate Immune Response in Intestinal Epithelial Cells and Macrophages

Elean, Mariano; Albarracín, Leonardo; Fukuyama, Kohtaro; Zhou, Bingwen; Tomokiyo, Mikado; Kitahara, Shugo; Araki, Shun; Suda, Yoshihito; Saavedra, Lucila; Villena, Julio; Hébert, Elvira María; Kitazawa, Haruki

doi:10.3390/microorganisms9122449

Cited by 5 publications

(6 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar behavior regulating the response to TLR3 activation in Raw 264.7 macrophages was observed with L. delbrueckii spp. lactis CRL 581 that significantly improved the expression of type I IFNs, IFN-γ, antiviral factors, TNF-α, and IL-1β, as well as increased IL-10 [48].…”

Section: Discussionmentioning

confidence: 97%

Lacticaseibacillus rhamnosus CRL1505 Peptidoglycan Modulates the Inflammation-Coagulation Response Triggered by Poly(I:C) in the Respiratory Tract

Zelaya,

Arellano-Arriagada,

Fukuyama

et al. 2023

IJMS

Self Cite

View full text Add to dashboard Cite

Lacticaseibacillus rhamnosus CRL1505 beneficially modulates the inflammation-coagulation response during respiratory viral infections. This study evaluated the capacity of the peptidoglycan obtained from the CRL1505 strain (PG-Lr1505) to modulate the immuno-coagulative response triggered by the viral pathogen-associated molecular pattern poly(I:C) in the respiratory tract. Adult BALB/c mice were nasally treated with PG-Lr1505 for two days. Treated and untreated control mice were then nasally challenged with poly(I:C). Mice received three doses of poly(I:C) with a 24 h rest period between each administration. The immuno-coagulative response was studied after the last administration of poly(I:C). The challenge with poly(I:C) significantly increased blood and respiratory pro-inflammatory mediators, decreased prothrombin activity (PT), and increased von Willebrand factor (vWF) levels in plasma. Furthermore, tissue factor (TF), tissue factor pathway inhibitor (TFPI), and thrombomodulin (TM) expressions were increased in the lungs. PG-Lr1505-treated mice showed significant modulation of hemostatic parameters in plasma (PT in %, Control = 71.3 ± 3.8, PG-Lr1505 = 94.0 ± 4.0, p < 0.01) and lungs. Moreover, PG-Lr1505-treated mice demonstrated reduced TF in F4/80 cells from lungs, higher pro-inflammatory mediators, and increased IL-10 compared to poly(I:C) control mice (IL-10 in pg/mL, Control = 379.1 ± 12.1, PG-Lr1505 = 483.9 ± 11.3, p < 0.0001). These changes induced by PG-Lr1505 correlated with a significant reduction in lung tissue damage. Complementary in vitro studies using Raw 264.7 cells confirmed the beneficial effect of PG-Lr1505 on poly(I:C)-induced inflammation, since increased IL-10 expression, as well as reduced damage, production of inflammatory mediators, and hemostatic parameter expressions were observed. In addition, protease-activated receptor-1 (PAR1) activation in lungs and Raw 264.7 cells was observed after TLR3 stimulation, which was differentially modulated by PG-Lr1505. The peptidoglycan from L. rhamnosus CRL1505 is able to regulate inflammation, the procoagulant state, and PAR1 activation in mice and macrophages in the context of the activation of TLR3 signaling pathways, contributing to a beneficial modulation of inflammation-hemostasis crosstalk.

show abstract

Section: Discussionmentioning

confidence: 97%

Lacticaseibacillus rhamnosus CRL1505 Peptidoglycan Modulates the Inflammation-Coagulation Response Triggered by Poly(I:C) in the Respiratory Tract

Zelaya,

Arellano-Arriagada,

Fukuyama

et al. 2023

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…On the other hand, probiotic bacteria can stimulate macrophages by increasing their phagocytic activities and their capacity to produce cytokines like IFN-γ and TNF-α. This macrophage activity is essential for the protection against infections [ 52 ]. Thus, our results suggest that both CRL681 and CRL1506 strains have the capacity to interact with IECs and macrophages in the gut, reinforcing the epithelial defenses and stimulating immune responses.…”

Section: Discussionmentioning

confidence: 99%

Lactiplantibacillus plantarum Strains Modulate Intestinal Innate Immune Response and Increase Resistance to Enterotoxigenic Escherichia coli Infection

et al. 2022

Self Cite

View full text Add to dashboard Cite

Currently, probiotic bacteria with not transferable antibiotic resistance represent a sustainable strategy for the treatment and prevention of enterotoxigenic Escherichia coli (ETEC) in farm animals. Lactiplantibacillus plantarum is among the most versatile species used in the food industry, either as starter cultures or probiotics. In the present work, the immunobiotic potential of L. plantarum CRL681 and CRL1506 was studied to evaluate their capability to improve the resistance to ETEC infection. In vitro studies using porcine intestinal epithelial (PIE) cells and in vivo experiments in mice were undertaken. Expression analysis indicated that both strains were able to trigger IL-6 and IL-8 expression in PIE cells in steady-state conditions. Furthermore, mice orally treated with these strains had significantly improved levels of IFN-γ and TNF-α in the intestine as well as enhanced activity of peritoneal macrophages. The ability of CRL681 and CRL1506 to beneficially modulate intestinal immunity was further evidenced in ETEC-challenge experiments. In vitro, the CRL1506 and CRL681 strains modulated the expression of inflammatory cytokines (IL-6) and chemokines (IL-8, CCL2, CXCL5 and CXCL9) in ETEC-stimulated PIE cells. In vivo experiments demonstrated the ability of both strains to beneficially regulate the immune response against this pathogen. Moreover, the oral treatment of mice with lactic acid bacteria (LAB) strains significantly reduced ETEC counts in jejunum and ileum and prevented the spread of the pathogen to the spleen and liver. Additionally, LAB treated-mice had improved levels of intestinal IL-10 both at steady state and after the challenge with ETEC. The protective effect against ETEC infection was not observed for the non-immunomodulatory TL2677 strain. Furthermore, the study showed that L. plantarum CRL1506 was more efficient than the CRL681 strain to modulate mucosal immunity highlighting the strain specific character of this probiotic activity. Our results suggest that the improved intestinal epithelial defenses and innate immunity induced by L. plantarum CRL1506 and CRL681 would increase the clearance of ETEC and at the same time, protect the host against detrimental inflammation. These constitute valuable features for future probiotic products able to improve the resistance to ETEC infection.

show abstract

“…mundtii CRL 35, , Lactobacillus delbrueckii subsp. lactis CRL 581, , and Levilactobacillus brevis CRL 2013 . Each LAB strain was cultured in a chemically defined medium (CDM) or in a serum-free and antibiotic-free RPMI 1640 medium.…”

Section: Methodsmentioning

confidence: 99%

“…The LAB strains used in this study, selected for their beneficial properties, belong to the CERELA culture collection (CERELA-CONICET); E. mundtii CRL 35, 23 , 38 Lactobacillus delbrueckii subsp. lactis CRL 581, 21 , 39 and Levilactobacillus brevis CRL 2013. 16 Each LAB strain was cultured in a chemically defined medium (CDM) 40 or in a serum-free and antibiotic-free RPMI 1640 medium.…”

Section: Methodsmentioning

confidence: 99%

“…lactis CRL 581 was included, because of its ability to reduce the expression of inflammatory cytokines in porcine intestinal epithelial cells and modulate cytokine expression in RAW 264.7, a macrophage-like cell line. 21 Enterococcus mundtii CRL 35 was the third strain chosen for this study due to its marked modulation of the cytokine profile produced by enterocytes in a listeriosis model (Saavedra, unpublished results). Recent evidence points out that bacterial or viral infections might be associated with vascular alterations in the onset of AD.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Improvement of Key Molecular Events Linked to Alzheimer’s Disease Pathology Using Postbiotics

Bulacios,

Cataldo,

Naja

et al. 2023

ACS Omega

Self Cite

View full text Add to dashboard Cite

In the past 50 years, life expectancy has increased by more than 20 years. One consequence of this increase in longevity is the rise of age-related diseases such as dementia. Alzheimer’s disease (AD) is the most common form of dementia, accounting for 60–70% of cases. AD pathogenesis is not restricted to the neuronal compartment but includes strong interactions with other brain cells, particularly microglia triggering the release of inflammatory mediators, which contribute to disease progression and severity. There is growing evidence revealing the diverse clinical benefits of postbiotics in many prevalent conditions, including neurodegenerative diseases. Here, we tested the ability of bacterial conditioned media (BCM) derived from selected lactic acid bacteria (LAB) strains to regulate core mechanisms relevant to AD pathophysiology in the microglia cell line BV-2. Levilactobacillus brevis CRL 2013, chosen for its efficient production of the neurotransmitter GABA, and Lactobacillus delbrueckii subsp. lactis CRL 581, known for its anti-inflammatory properties, were selected alongside Enterococcus mundtii CRL 35, a LAB strain that can significantly modulate cytokine production. BCM from all 3 strains displayed antioxidant capabilities, reducing oxidative stress triggered by beta-amyloid oligomers (oAβ 1–42 ). Additionally, BCM effectively mitigated the expression of inflammatory cytokines, namely, TNF-α, IL-1β, and IL-6 triggered by oAβ 1–42 . Furthermore, our study identified that BCM from CRL 581 inhibit the activity of acetylcholinesterase (AChE), a crucial enzyme in AD progression, in both human erythrocytes and mouse brain tissues. Notably, the inhibitory effect was mediated by low-molecular-weight components of the BCM. L. delbrueckii subsp. lactis CRL 581 emerged as a favorable candidate for production of postbiotics with potential benefits for AD therapy since it demonstrated potent antioxidant activity, reduction of cytokine expression, and partial AChE inhibition. On the other hand, E. mundtii CRL 35 showed that the antioxidant activity failed to inhibit AChE and caused induction of iNOS expression, rendering it unsuitable as a potential therapeutic for AD. This study unveils the potential benefits of LAB-derived postbiotics for the development of new avenues for therapeutic interventions for AD.

show abstract

Lactobacillus delbrueckii CRL 581 Differentially Modulates TLR3-Triggered Antiviral Innate Immune Response in Intestinal Epithelial Cells and Macrophages

Cited by 5 publications

References 68 publications

Lacticaseibacillus rhamnosus CRL1505 Peptidoglycan Modulates the Inflammation-Coagulation Response Triggered by Poly(I:C) in the Respiratory Tract

Lacticaseibacillus rhamnosus CRL1505 Peptidoglycan Modulates the Inflammation-Coagulation Response Triggered by Poly(I:C) in the Respiratory Tract

Lactiplantibacillus plantarum Strains Modulate Intestinal Innate Immune Response and Increase Resistance to Enterotoxigenic Escherichia coli Infection

Improvement of Key Molecular Events Linked to Alzheimer’s Disease Pathology Using Postbiotics

Contact Info

Product

Resources

About