Lactobacillus casei DN-114 001 inhibits the increase in paracellular permeability of enteropathogenic Escherichia coli-infected T84 cells

Parassol, Nadège; Freitas, Miguel; Thoreux, Karine; Dalmasso, Guillaume; Bourdet‐Sicard, Raphaëlle; Rampal, Patrick

doi:10.1016/j.resmic.2004.09.013

Cited by 123 publications

(99 citation statements)

References 27 publications

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“…Infection models used by other investigators demonstrated that both probiotic mixtures (such as VSL#3) and additional single strains (e.g., E. coli Nissle 1917 and L. casei DN-114 001) prevent ZO-1 redistribution in response to Salmonella enterica serovar Dublin and enteropathogenic E. coli infections in vitro (30,31). In our study, L. rhamnosus GG pretreatment prior to E. coli O157:H7 infection ameliorated the pathogen-induced redistribution of ZO-1.…”

Section: Discussionsupporting

confidence: 59%

Lactobacillus rhamnosus Strain GG Prevents Enterohemorrhagic Escherichia coli O157:H7-Induced Changes in Epithelial Barrier Function

et al. 2008

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Enterohemorrhagic Escherichia coli (EHEC) O157:H7 intimately attaches to intestinal epithelial monolayers and produces attaching and effacing (A/E) lesions. In addition, EHEC infection causes disruptions of intercellular tight junctions, leading to clinical sequelae that include acute diarrhea, hemorrhagic colitis, and the hemolytic-uremic syndrome. Current therapy remains supportive since antibiotic therapy increases the risk of systemic complications. This study focused on the potential therapeutic effect of an alternative form of therapy, probiotic Lactobacillus rhamnosus strain GG, to attenuate EHEC-induced changes in paracellular permeability in polarized MDCK-I and T84 epithelial cell monolayers. Changes in epithelial cell morphology, electrical resistance, dextran permeability, and distribution and expression of claudin-1 and ZO-1 were assessed using phase-contrast, immunofluorescence, and transmission electron microscopy and macromolecular flux. This study demonstrated that pretreatment of polarized MDCK-I and T84 cells with the probiotic L. rhamnosus GG reduced morphological changes and diminished the number of A/E lesions induced in response to EHEC O157:H7 infection. With probiotic pretreatment there was corresponding attenuation of the EHEC-induced drop in electrical resistance and the increase in barrier permeability assays. In addition, L. rhamnosus GG protected epithelial monolayers against EHEC-induced redistribution of the claudin-1 and ZO-1 tight junction proteins. In contrast to the effects seen with the live probiotic, heat-inactivated L. rhamnosus GG had no effect on EHEC binding and A/E lesion formation or on disruption of the barrier function. Collectively, these findings provide in vitro evidence that treatment with the probiotic L. rhamnosus strain GG could prove to be an effective management treatment for preventing injury of the epithelial cell barrier induced by A/E bacterial enteropathogens.

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Section: Discussionsupporting

confidence: 59%

Lactobacillus rhamnosus Strain GG Prevents Enterohemorrhagic Escherichia coli O157:H7-Induced Changes in Epithelial Barrier Function

et al. 2008

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“…The results of this study provide a highly plausible explanation for the reported protective effects of L. plantarum and other probiotic strains on barrier disruption by inflammatory cytokines, chemicals, and infectious agents (13,18,20,28,32,34,35,48). However, increased mucin production, immunomodulation, pathogen inhibition, and effects on the resident microbiota could also be contributory factors, particularly in vivo.…”

Section: G855mentioning

confidence: 59%

“…In addition, there is evidence from in vitro studies that particular strains of Lactobacillus spp. can protect against intestinal barrier dysfunctions caused by invasive pathogens or proinflammatory cytokines (13,28,34,35).…”

Section: Translational Highlights This Study Demonstrates That In Thmentioning

confidence: 99%

Regulation of human epithelial tight junction proteins by Lactobacillus plantarum in vivo and protective effects on the epithelial barrier

Karczewski

Troost

Konings

et al. 2010

American Journal of Physiology-Gastrointestinal and Liver Physi

528

390

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Karczewski J, Troost FJ, Konings I, Dekker J, Kleerebezem M, Brummer RM, Wells JM. Regulation of human epithelial tight junction proteins by Lactobacillus plantarum in vivo and protective effects on the epithelial barrier. Am J Physiol Gastrointest Liver Physiol 298: G851-G859, 2010. First published March 11, 2010; doi:10.1152/ajpgi.00327.2009.-Lactobacillus plantarum, a commensal bacterium of humans, has been proposed to enhance the intestinal barrier, which is compromised in a number of intestinal disorders. To study the effect of L. plantarum strain WCFS1 on human barrier function, healthy subjects were administered L. plantarum or placebo in the duodenum for 6 h by means of a feeding catheter. The scaffold protein zonula occludens (ZO)-1 and transmembrane protein occludin were found to be significantly increased in the vicinity of the tight-junction (TJ) structures, which form the paracellular seal between cells of the epithelium. In an in vitro model of the human epithelium, L. plantarum induced translocation of ZO-1 to the TJ region; however, the effects on occludin were minor compared with those seen in vivo. L. plantarum was shown to activate Toll-like receptor 2 (TLR2) signaling, and treatment of Caco-2 monolayers with the TLR2 agonist Pam 3 -Cys-SK4(PCSK) significantly increased fluorescent staining of occludin in the TJ. Pretreatment of Caco-2 monolayers with L. plantarum or PCSK significantly attenuated the effects of phorbol ester-induced dislocation of ZO-1 and occludin and the associated increase in epithelial permeability. Our results identifying commensal bacterial stimulation of TLR2 in the gut epithelium as a regulator of epithelial integrity have important implications for understanding probiotic mechanisms and the control of intestinal homeostasis. epithelium; intestine

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“…Studies using intestinal epithelial cells have suggested that probiotic treatment of IEC stimulates mucin secretion (43) and the enhancement of epithelial barrier functions (56), reduces enterocyte apoptosis (32,40), and stabilizes formation of cellular TJs (51,52). Despite recent advances in understanding probiotic actions on IEC, little is known about the mechanisms of protection from in vivo models of gastrointestinal diseases.…”

Section: Discussionmentioning

confidence: 99%

Bifidobacterium bifidum improves intestinal integrity in a rat model of necrotizing enterocolitis

Khailová¹,

Dvorak

Arganbright³

et al. 2009

American Journal of Physiology-Gastrointestinal and Liver Physi

164

159

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Lactobacillus casei DN-114 001 inhibits the increase in paracellular permeability of enteropathogenic Escherichia coli-infected T84 cells

Cited by 123 publications

References 27 publications

Lactobacillus rhamnosus Strain GG Prevents Enterohemorrhagic Escherichia coli O157:H7-Induced Changes in Epithelial Barrier Function

Lactobacillus rhamnosus Strain GG Prevents Enterohemorrhagic Escherichia coli O157:H7-Induced Changes in Epithelial Barrier Function

Regulation of human epithelial tight junction proteins by Lactobacillus plantarum in vivo and protective effects on the epithelial barrier

Bifidobacterium bifidum improves intestinal integrity in a rat model of necrotizing enterocolitis

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