Lactobacillus acidophilus LA 1 binds to cultured human intestinal cell lines and inhibits cell attachment and cell invasion by enterovirulent bacteria.

Abstract

Four human Lactobacillus acidophilus strains were tested for their ability to adhere onto human enterocyte like Caco-2 cells in culture. The LA 1 strain exhibited a high calcium independent adhesive property. This adhesion onto Caco-2 cells required a proteinaceous adhesion promoting factor, which was present in the spent bacterial broth culture supernatant. LA 1 strain also strongly bound to the mucus secreted by the homogeneous cultured human goblet cell line HT29-MTX. The inhibitory effect of LA 1 organisms against Caco-2 cell adhesion and cell invasion by a large variety of diarrhoeagenic bacteria was investigated. As a result, the following dose dependent inhibitions were obtained: (a) against the cell association of enterotoxigenic, diffusely adhering and enteropathogenic Escherichia coli, and Salmonella typhimurium; (b) against the cell invasion by enteropathogenic Eschericha coli, Yersinia pseudotuberculosis, and Salmonella typhimurium. Incubations of L acidophilus LA 1 before and together with enterovirulent E coli were more effective than incubation after infection by E coli. (Gut 1994; 35: 483-489) Equipe de Pathogenie Microbienne Cellulaire et Moleculaire Intestinale,

Lactic acid bacteria protect human intestinal epithelial cells from Staphylococcus aureus and Pseudomonas aeruginosa infections

“…Therefore, it can be hypothesized that competition for the binding site is a major mechanism through which lactic acid bacteria antagonize S. aureus and P. aeruginosa. Lactic acid bacteria counteract the adhesive and invasive function of pathogenic bacteria by causing a nonspecific steric hindrance action on the pathogenic receptors (Bernet et al, 1993;Bernet et al, 1994), and by producing inhibitory metabolites. Coconnier-Polter et al (2005) have previously reported that the CFS of L. acidophilus could potentially decrease the intracellular ATP content in S. enterica SL1344.…”

Lactic acid bacteria and the human gastrointestinal tract

“…Other studies have demonstrated that lactic acid bacteria stimulate macrophage phagocytosis of viable Salmonella (Hatcher & Lambrecht, 1993), enhance IgA production in intestinal secretions (Perdigon et al, 1990), produce an antimicrobial substance (Shahani & Ayebo, 1980;Silvia et al, 1987), inhibit cell attachment and cell invasion by enterovirulent bacteria (Bernet et al, 1994), and decrease intestinal permeability for macromolecules during rotavirus induced diarrhoea (Isolauri et al, 1993).…”

13C- and 15N-incorporation of doubly stable isotope labelled Lactobacillus johnsonii in humans

“…Lactobacilli are prominent members of the microbiota. Lactobacillus johnsonii (La1), formerly known as L. acidophilus (Finegold et al, 1983) is a Lactobacillus strain of human origin that has been selected because of its ability to adhere to human intestinal mucosa (Bernet et al, 1994). Human in vivo studies have proven that La1 supports the physiological function of the gastrointestinal tract (GIT) and stimulates the immune defense (Pfeiffer & Rosat, 1999).…”