Lactobacillus acidophilus DDS-1 Modulates the Gut Microbiota and Improves Metabolic Profiles in Aging Mice

Abstract

Recent evidence suggests that gut microbiota shifts can alter host metabolism even during healthy aging. Lactobacillus acidophilus DDS-1, a probiotic strain, has shown promising probiotic character in vitro, as well as in clinical studies. The present study was carried out to investigate whether DDS-1 can modulate the host metabolic phenotype under the condition of age-affected gut microbial shifts in young and aging C57BL/6J mice. Collected fecal samples were analyzed using 16S rRNA gene sequencing for identifying gut microbiota and untargeted gas chromatography-mass spectrometry (GC-MS) metabolomics analysis. Gut microbial shifts were observed in the control groups (young and aging), leading to an alteration in metabolism. Principal coordinate analysis (PCoA) of microbiota indicated distinct separation in both the DDS-1-treated groups. L. acidophilus DDS-1 increased the relative abundances of beneficial bacteria, such as Akkermansia muciniphila and Lactobacillus spp., and reduced the relative levels of opportunistic bacteria such as Proteobacteria spp. Metabolic pathway analysis identified 10 key pathways involving amino acid metabolism, protein synthesis and metabolism, carbohydrate metabolism, and butanoate metabolism. These findings suggest that modulation of gut microbiota by DDS-1 results in improvement of metabolic phenotype in the aging mice.

Lactobacillus acidophilus DDS-1 and Bifidobacterium lactis UABla-12 Improve Abdominal Pain Severity and Symptomology in Irritable Bowel Syndrome: Randomized Controlled Trial

“…The gut microbiota also plays an important role in the balance between immunosuppression and inflammation, involving Toll-like receptor signaling pathways [39]. L. acidophilus DDS-1 was previously shown to help modulate the fecal and mucosal microbiota in young and aging mice, while downregulating the production of inflammatory cytokines in serum and colonic explants [17,18]. Additionally, L. acidophilus DDS-1, in combination with B. lactis UABla-12, displayed improved symptomology associated with atopic dermatitis in a randomized controlled trial, with modulation of blood lymphocyte subsets (i.e., CD4, CD8 and CD25) suggesting an immune regulatory role [15].…”

“…L. acidophilus DDS-1, alone or in combination with B. lactis UABla-12, has previously been shown to normalize bowel habits in functional constipation [12], provide abdominal symptom relief in lactose intolerance [13] and support immune specific outcomes [14,15] in randomized controlled trials. Mechanistically, L. acidophilus DDS-1 has exhibited immunomodulatory capacity in vitro [16], while both modulating the microbiota and downregulating the inflammatory profile in pre-clinical models [17,18]. Additionally, L. acidophilus DDS-1 in combination with B. lactis UABla-12 has demonstrated an immune regulatory role in support of immune specific outcomes clinically [15].…”

Functional microbiome deficits associated with ageing: Chronological age threshold

“…However, the “aged” microbiota in elderly individuals does not produce these molecules. A number of dietary factors and nutritional interventions with other products exert positive effects on the elderly (Ticinesi et al, ; Cortés‐Martín et al, ; Grosicki, Fielding, & Lustgarten, ; Sandgren & Brummer, ; Vemuri et al, ; Wu et al, ; Gao, Zhang, Huang, Shen, & Qin, ; Jones et al, ; Tran et al, ) or extend the lifespan of rodents (Miller et al, ). Thus, we are proposing a new intervention.…”

Modulating the Microbiome and Immune Responses Using Whole Plant Fibre in Synbiotic Combination with Fibre-Digesting Probiotic Attenuates Chronic Colonic Inflammation in Spontaneous Colitic Mice Model of IBD

“…DNA extracted from C57BL/6J wild-type (WT) mice were also used for comparison. The samples underwent high-throughput sequencing on the Illumina MiSeq platform at the Australian Genome Research Facility (University of Queensland, Brisbane, QLD, Australia) according to the methods detailed earlier [ 32 ]. Polymerase chain reaction (PCR) amplicons spanning the 16 S rRNA V1–V3 hypervariable region with 27 F forward primer (5′-AGAGTTTGATCMTGGCTCAG-3′) and 519 R reverse primer (5′-GWATTACCGCGGCKGCTG-3′) were sequenced.…”

“…GC-MS analysis of 100–150 mg fresh weight (stored at −80 °C) of caecal, mucosal-associated and faecal samples ( n = 5 per group) from Winnie and WT mice was conducted for volatile SCFA profiling following the method described previously [ 27 , 32 ]. Briefly, following sample preparation, the GC-MS analysis was performed on an Agilent 6890B GC oven coupled to a 5977B mass spectrometer (MS) detector (Agilent Technologies, Mulgrave, VIC, Australia) fitted with an MPS autosampler (Gerstel GmbH & Co.KG, Mülheim an der Ruhr, Germany).…”