Lactobacilli isolated from vaginal vault of dairy and meat cows during progesteronic stage of estrous cycle

Rodríguez, Constanza Montalvo; Cofre, Jaime; Sánchez, M.; Fernández, P; Boggiano, Gioconda; Castro, Erica

doi:10.1016/j.anaerobe.2010.12.001

Cited by 23 publications

(16 citation statements)

References 23 publications

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“…were prevalent, but at low relative abundances among cow and ewe vaginal microbiota. This is consistent with previously reported culture-driven results of the cow vagina ( 6 – 8 ). Lactobacillus delbrueckii was one Lactobacillus species detected in both cow and ewe vaginas (Table 2 ), which has previously been isolated from the cow vagina ( 8 ).…”

Section: Discussionsupporting

confidence: 93%

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Characterization of the Vaginal Microbiota of Ewes and Cows Reveals a Unique Microbiota with Low Levels of Lactobacilli and Near-Neutral pH

et al. 2014

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Although a number of common reproductive disorders in livestock involve bacterial infection, very little is known about their normal vaginal microbiota. Therefore, we sought to determine the species composition of sheep and cattle vaginal microbiota. Twenty Rambouillet ewes and twenty crossbred cows varying in age and reproductive status were sampled by ectocervicovaginal lavage. We amplified and sequenced the V3–V4 region of the 16S ribosomal RNA (rRNA) contents yielding a total of 907,667 high-quality reads. Good’s Coverage estimates indicated that we obtained data on 98 ± 0.01% of the total microbial genera present in each sample. Cow and ewe vaginal microbiota displayed few differences. Cow microbiota exhibited greater (P ≤ 0.05) α-diversity compared to the ewe microbiota. Both livestock species differed (P ≤ 0.05) from all previously reported vaginal communities. While bacteria were numerically dominant, Archaea were detected in 95% of cow and ewe samples, mainly of the order Desulfurococcales. Both ewes and cows were predominately colonized by the bacterial phyla Bacteroidetes, Fusobacteria, and Proteobacteria. The most abundant genera were Aggregatibacter spp., and Streptobacillus spp. Lactobacillus spp. were detected in 80% of ewe and 90% of cow samples, but only at very low abundances. Bacteria previously described from culture-based studies as common to the cow and ewe vaginal tract, except for Escherichia, were variably present, and only in low abundance. Ewe and cow pH differed (P ≤ 0.05), with means (±SD) of 6.7 ± 0.38 and 7.3 ± 0.63, respectively. In conclusion, 16S rRNA sequencing of cow and ewe vaginal ectocervicovaginal lavages showed that cow and ewe vaginal microbiota differ from culture-led results, revealing a microbiota distinct from previously described vaginal ecosystems.

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Section: Discussionsupporting

confidence: 93%

“…Similarly, the few culture-based studies that have been conducted on livestock have reported Lactobacillus spp. at lower abundances than other microbial genera in both the cow and ewe vagina ( 6 – 8 ). Enterococcus spp., Staphylococcus spp., and Streptococcus spp.…”

Section: Introductionmentioning

confidence: 96%

Characterization of the Vaginal Microbiota of Ewes and Cows Reveals a Unique Microbiota with Low Levels of Lactobacilli and Near-Neutral pH

et al. 2014

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“…Few studies have mentioned the culture of lactobacilli from uterine samples of cows [ 5 , 6 ], but their role in the uterine environment has not yet been elucidated. In the bovine vagina, the presence of lactobacilli has been shown during the growth of healthy heifers [ 7 ] and during the luteal phase of the oestrous cycle [ 8 ]. Lactobacilli are predominant in the human vaginal tract [ 9 ], where they play a role in maintaining the vaginal ecosystem in a healthy status by producing hydrogen peroxide, acetic and lactic acid and antibacterial molecules for a first defence against bacteria [ 10 – 12 ].…”

Section: Introductionmentioning

confidence: 99%

Detection and Characterisation of Lactobacillus spp. in the Bovine Uterus and Their Influence on Bovine Endometrial Epithelial Cells In Vitro

Gärtner

Bondzio

Braun³

et al. 2015

PLoS ONE

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Bacterial infections and inflammation of the uterus are common in dairy cattle after parturition. In particular, pathogenic bacteria that cause endometritis have been the focus of research in cattle reproduction in the last ten years. The aim of the present study was to identify commensal lactobacilli in the bovine uterus and to examine their influence on the synthesis of pro-inflammatory factors in bovine endometrial epithelial cells in vitro. Lactobacillus species were isolated from healthy bovine uteri and further characterised. Bovine endometrial epithelial cells in the second passage (n = 5 animals) were co-cultured with the autochthonous isolates L. buchneri, L. ruminis and L. amylovorus as well as with a commercially available L. vaginalis in different multiplicities of infection (MOI = 1, 5 and 10, respectively). Endometrial epithelial cells cultured without bacteria served as controls. At distinct points in time (2, 4 and 6 h) total RNA was extracted from co-cultured epithelial cells and subjected to reverse transcription quantitative PCR of pro-inflammatory factors. Furthermore, the release of such factors by co-cultured epithelial cells was measured by ELISA or EIA after 24 and 48 h. L. ruminis and L. amylovorus induced increased interleukin (IL) IL1A, IL6, IL8 and prostaglandin-endoperoxide synthase 2 mRNA levels and the release of IL8 and prostaglandin F2α in endometrial epithelial cells compared with control cells. In contrast, L. buchneri did not significantly influence the expression and release of these factors. Toll-like receptors 2 and 6 transcripts were found unchanged in co-cultured and untreated epithelial cells in vitro. However, endometrial epithelial cells of each animal showed individual differences in the response to bacterial load. These results suggest that Lactobacillus species are present in the bovine uterus, revealing immunomodulatory properties.

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“…Production of H 2 O 2 is measured as one of the protection mechanisms of lactobacilli against vaginal infections (Pascual et al, 2006). Results of this study revealed that only four LAB isolates (22.2%) were H 2 O 2 -producing, which is lower than the 45% H 2 O 2 -producing vaginal lactobacilli isolated from cattle (Rodriguez et al, 2011) and the 96% (Eschenbach et al, 1989) and 62% (Pascual et al, 2006) isolated from women.…”

Section: Discussionmentioning

confidence: 82%

“…The ability of LAB to produce hydrogen peroxide (H 2 O 2 ) was qualitatively assessed by streaking the isolates on MRS agar containing tetramethyl-benzidine (TMB) and horseradish peroxidase (Sigma-Aldrich, Seelze, Germany). The plates were incubated anaerobically at 37 C for 48 h. Colonies that had produced H 2 O 2 appeared dark blue (Rodriguez et al, 2011). All bacteriological culture media were subjected to quality control before use (Weenk et al, 1992) and uncultured media were included with each test as a negative control to ensure the sterility.…”

Section: Lactobacillus Isolation and Characterizationmentioning

confidence: 99%

Isolation and characterization of vaginal Lactobacillus spp. in dromedary camels (Camelus dromedarius): in vitro evaluation of probiotic potential of selected isolates

El-Deeb

Fayez²,

Elsohaby

et al. 2020

PeerJ

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Lactobacillus spp. is one of the beneficial lactic acid producing microbiota in the vagina, which is important for a healthy vaginal environment. However, little is known about vaginal Lactobacillus in dromedary camels (Camelus dromedarius). Therefore, this study aimed to isolate vaginal lactic acid bacteria (LAB) in dromedary camels and to study the probiotic potential of selected isolates. A total of 75 vaginal swabs were collected from pluriparous, non-pregnant, non-lactating dromedary camels. The LAB were isolated using deMan, Rogosa and Sharpe broth and agar media. Suspected LAB isolates were subjected to catalase testing and Gram staining and examined for indole production, nitrate reduction, hemolytic activity, cell surface hydrophobicity, auto- and coaggregation, antibacterial activity and characterized by 16S rRNA amplification and sequencing. Eighteen LABs were isolated from the 75 vaginal swabs. Among the 18 LAB isolates, six were Lactobacillus plantarum, eight were Lactobacillus fermentum, and four were Lactobacillus rhamnosus. None of the LAB isolates was hemolytic and only four LAB were H2O2 producing. The percentage of hydrophobicity ranged from 0% to 49.6%, 0% to 44.3% and 0% to 41.6% for hexadecane, xylene and toluene, respectively. All isolates showed higher (P < 0.05) autoaggregation after 24 h of incubation compared to 4 h. Furthermore, all LAB showed higher coaggregation (P < 0.05) and antimicrobial activity toward Staphylococcus aureus than to Escherichia coli. All LAB isolates were vancomycin resistant and sensitive to streptomycin, erythromycin, kanamycin and chloramphenicol. Only, three LAB isolates were resistant to tetracycline. The dromedary camel vaginal LAB isolates exhibited varying degrees of in vitro probiotic properties tested in this study and showed promising activity against the most common bacterial causes of endometritis in dromedary camels. Further investigation of the in vivo effect of these isolates is warranted.

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Lactobacilli isolated from vaginal vault of dairy and meat cows during progesteronic stage of estrous cycle

Cited by 23 publications

References 23 publications

Characterization of the Vaginal Microbiota of Ewes and Cows Reveals a Unique Microbiota with Low Levels of Lactobacilli and Near-Neutral pH

Characterization of the Vaginal Microbiota of Ewes and Cows Reveals a Unique Microbiota with Low Levels of Lactobacilli and Near-Neutral pH

Detection and Characterisation of Lactobacillus spp. in the Bovine Uterus and Their Influence on Bovine Endometrial Epithelial Cells In Vitro

Isolation and characterization of vaginal Lactobacillus spp. in dromedary camels (Camelus dromedarius): in vitro evaluation of probiotic potential of selected isolates

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