Lactobacilli and Streptococci Induce Interleukin-12 (IL-12), IL-18, and Gamma Interferon Production in Human Peripheral Blood Mononuclear Cells

Miettinen, Minja; Matikainen, Sampsa; Vuopio‐Varkila, Jaana; Pirhonen, Jaana; Varkila, K; Kurimoto, Masashi; Julkunen, Ilkka

doi:10.1128/iai.66.12.6058-6062.1998

Cited by 294 publications

(128 citation statements)

References 40 publications

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“…All Lactobacillus strains tested in our study stimulated IL-10, IFN-g and TNF-a production both after 1 and 4 days of culture as well as IL-1b after 1 day of culture. In support of our results, Lactobacillus strains are generally strong inducers of the proinflammatory cytokines TNF-a (Miettinen et al, 1998;Maragkoudakis et al, 2006;Timmerman et al, 2007) and IL-12 (Miettinen et al, 1998;Hessle et al, 1999;Haller et al, 2000;Shida et al, 2006), although several studies observed that specific lactic acid bacteria yield a low or nonsignificant increase in IL-12 production by hPBMC (Niers et al, 2005;Drouault-Holowacz et al, 2006). In our study, all L. plantarum strains tested stimulated IL-12 production, while most L. acidophilus strains failed to do so, which confirms that the difference in IL-12 induction observed in the reported studies is caused by the use of different species and strains.…”

Section: Discussionsupporting

confidence: 89%

Differential effects ofLactobacillus acidophilusandLactobacillus plantarumstrains on cytokine induction in human peripheral blood mononuclear cells

Vissers

Snel

Zuurendonk

et al. 2010

FEMS Immunol Med Microbiol

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Lactic acid bacterial strains have received interest for their immunomodulating activities and potential use in probiotic products. A wide variety of strain-dependent properties have been reported, but comparative studies at the species level are scarce. The objective of this study was to assess the immunomodulatory effect of Lactobacillus species on the cytokine profiles and proliferative response of human peripheral blood mononuclear cells (hPBMC), and in particular, on the comparison between the species Lactobacillus acidophilus and Lactobacillus plantarum. hPBMC from healthy donors were stimulated in the presence or absence of the lactic acid bacteria, and cytokine production, surface marker staining, proliferation and cell death were determined after 1 and 4 days of culture. All Lactobacillus strains tested were capable of inducing the production of interleukin (IL)-1beta, IL-10, interferon-gamma (IFN-gamma) and tumor necrosis factor-alpha (TNF-alpha). The bacterial strains did not differentially influence the amount of proliferating, viable, apoptotic and necrotic cells. Generally, L. plantarum showed a significantly higher induction capacity of IFN-gamma, IL-12 and TNF-alpha compared with L. acidophilus. We conclude that the variation in immunomodulatory effects between species is even larger than the variation between the strains of the same species. In addition, we demonstrate that L. plantarum strains are most potent in skewing the T-cell differentiation toward a putative Th1 response.

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Section: Discussionsupporting

confidence: 89%

Differential effects ofLactobacillus acidophilusandLactobacillus plantarumstrains on cytokine induction in human peripheral blood mononuclear cells

Vissers

Snel

Zuurendonk

et al. 2010

FEMS Immunol Med Microbiol

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“…It is however evident from the obtained results that probably more than one mechanism may be involved in the observed antiviral effect, as previously hypothesized in similar studies (Botić et al, 2007;Ivec et al, 2007). In addition, apart from ROS and attachment, the release of pro-inflammatory cytokines as interleukins (IL) 6 and 12 or the antiviral interferon-γ (IFN-γ), is an important mechanism by which probiotic bacteria may protect host cells (Miettinen et al, 1998;Hessle et al, 1999;Maassen et al, 2000;Ivec et al, 2007). The mechanism of action of the antiviral effects reported in this study needs to be further investigated and so a more detailed, holistic approach would need to be adopted, focusing less on the screening of LAB strains against various cell lines and viruses, and more on in depth studies of the phenomenon.…”

Section: Discussionmentioning

confidence: 52%

Lactic acid bacteria efficiently protect human and animal intestinal epithelial and immune cells from enteric virus infection

Maragkoudakis

Chingwaru

Gradišnik

et al. 2010

International Journal of Food Microbiology

135

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This study aimed to examine the potential antiviral activity of lactic acid bacteria (LAB) using animal and human intestinal and macrophage cell line models of non tumor origin. To this end, LAB strains selected on the basis of previous in vitro trials were co-incubated with cell line monolayers, which were subsequently challenged with rotavirus (RV) and transmissible gastroenteritis virus (TGEV). In order to elucidate the possible mechanism responsible for the antiviral activity, the induction of reactive oxygen species (ROS) release as well as the attachment ability of LAB on the cell lines was investigated. Various strains were found to exhibit moderate to complete monolayer protection against viral RV or TGEV disruption. Highest protection effects were recorded with the known probiotics Lactobacillus rhamnosus GG and Lactobacillus casei Shirota against both RV and TGEV, while notable antiviral activity was also attributed to Enterococcus faecium PCK38, Lactobacillus fermentum ACA-DC179, Lactobacillus pentosus PCA227 and Lactobacillus plantarum PCA236 and PCS22, depending on the cell line and virus combination used. A variable increase (of up to 50%) on the release of NO(-) and H(2)O(2) (ROS) was obtained when LAB strains were co-incubated with the cell lines, but the results were found to be LAB strain and cell line specific, apart from a small number of strains which were able to induce strong ROS release in more than one cell line. In contrast, the ability of the examined LAB strains to attach to the cell line monolayers was LAB strain but not cell line specific. Highest attachment ability was observed with L. plantarum ACA-DC 146, L. paracasei subsp. tolerans ACA-DC 4037 and E. faecium PCD71. Clear indications on the nature of the antiviral effect were evident only in the case of the L. casei Shirota against TGEV and with L. plantarum PCA236 against both RV and TGEV. In the rest of the cases, each interaction was LAB-cell line-virus specific, barring general conclusions. However, it is probable that more than one mechanism is involved in the antiviral effect described here. Further investigations are required to elucidate the underlying mode of action and to develop a cell line model as a system for selection of probiotic strains suited for farm animal applications.

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“…We examined the mechanism by which L. plantarum induces TRAIL. Previous reports showed that TRAIL is expressed on immu- nocytes after stimulation with IFN-a [16], IFN-c [17], and that the administration of lactobacilli induced the release of IFN-c [18]. Therefore, we hypothesized that L. plantarum induces TRAIL expression through IFN-a and/or IFN-c. We examined the involvement of IFN-a and/or IFN-c in the expression of the TRAIL on the surface of PBMC ( Fig.…”

Section: Trail Induction By L Plantarum Partly Depends On the Producmentioning

confidence: 95%

Lactobacillus strains induce TRAIL production and facilitate natural killer activity against cancer cells

et al. 2009

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Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is an endogenous cytokine that induces apoptosis in malignant tumor cells. Here, we show for the first time that lactobacilli induce TRAIL production in human peripheral blood mononuclear cells (PBMC). Treatment with lactobacilli induced TRAIL on the cell surface of PBMC and in culture medium. The TRAIL production induced by lactobacilli partially depends on IFN-alpha and IFN-gamma. Lactobacilli treatment facilitated NK activity of PBMC against prostate cancer cells. Moreover, TRAIL neutralization antibody efficiently prevented the NK activity. Our results indicate that lactobacilli facilitate NK activity through TRAIL production, and raise the possibility of a new TRAIL-based strategy against malignant tumors.

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Lactobacilli and Streptococci Induce Interleukin-12 (IL-12), IL-18, and Gamma Interferon Production in Human Peripheral Blood Mononuclear Cells

Cited by 294 publications

References 40 publications

Differential effects ofLactobacillus acidophilusandLactobacillus plantarumstrains on cytokine induction in human peripheral blood mononuclear cells

Differential effects ofLactobacillus acidophilusandLactobacillus plantarumstrains on cytokine induction in human peripheral blood mononuclear cells

Lactic acid bacteria efficiently protect human and animal intestinal epithelial and immune cells from enteric virus infection

Lactobacillus strains induce TRAIL production and facilitate natural killer activity against cancer cells

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