Lacticaseibacillus paracasei GM-080 Ameliorates Allergic Airway Inflammation in Children with Allergic Rhinitis: From an Animal Model to a Double-Blind, Randomized, Placebo-Controlled Trial

Abstract

Background: Probiotics may facilitate the clinical management of allergic diseases. However, their effects on allergic rhinitis (AR) remain unclear. We examined the efficacy and safety of Lacticaseibacillus paracasei GM-080 in a mouse model of airway hyper-responsiveness (AHR) and in children with perennial AR (PAR) by using a double-blind, prospective, randomized, placebo-controlled design. Methods: The production of interferon (IFN)-γ and interleukin (IL)-12 was measured by using an enzyme-linked immunosorbent assay. GM-080 safety was evaluated via the whole-genome sequencing (WGS) of virulence genes. An ovalbumin (OVA)-induced AHR mouse model was constructed, and lung inflammation was evaluated by measuring the infiltrating leukocyte content of bronchoalveolar lavage fluid. A clinical trial was conducted with 122 children with PAR who were randomized to receive different doses of GM-080 or the placebo for 3 months, and their AHR symptom severity scores, total nasal symptom scores (TNSSs), and Investigator Global Assessment Scale scores were examined. Results: Among the tested L. paracasei strains, GM-080 induced the highest IFN-γ and IL-12 levels in mouse splenocytes. WGS analysis revealed the absence of virulence factors or antibiotic-resistance genes in GM-080. The oral administration of GM-080 at 1 × 107 colony forming units (CFU)/mouse/day for 8 weeks alleviated OVA-induced AHR and reduced airway inflammation in mice. In children with PAR, the oral consumption of GM-080 at 2 × 109 CFU/day for 3 months ameliorated sneezing and improved Investigator Global Assessment Scale scores significantly. GM-080 consumption led to a nonsignificant decrease in TNSS and also nonsignificantly reduced IgE but increased INF-γ levels. Conclusion: GM-080 may be used as a nutrient supplement to alleviate airway allergic inflammation.

Carbohydrate Hydrolytic Activity, Antibiotic Resistance and Stress Tolerance ofLacticaseibacillus paracaseiBCRC-16100 andLacticaseibacillus paracaseiZFM54 for Probiotics Using Genomic and Biochemical Approaches

“…The genus of the two strains is classified as probiotics but there is very little to know understanding on their health benefits, tolerance to stress and safety. Moreover, the species of the two strains are also recognized as probiotics only known for ameliorating allergic airway, antimicrobial activity, enhancement of intestinal microbiota and stress modulator, among others (Lin et al , 2023; Hill et al , 2018; and Jones, 2017). The intrinsic heterogeneity across strains of Lacticaseibacillus paracasei makes the species a strong group for the selection of probiotics isolates for health benefits and development of drugs or functional food for the abovementioned purpose (Balzaretti et al, 2017).…”

Probiotic Lactobacillus plantarum GUANKE effectively alleviates allergic rhinitis symptoms by modulating functions of various cytokines and chemokines

“…Similarly, oral administration of another Lactiplantibacillus plantarum NR16 reduced airway hyperresponsiveness and leukocyte infiltration in lesions of birch pollen-induced AR mice ( 22 ). Lin et al ( 23 ) applied Lacticaseibacillus paracasei GM-080 in both ovalbumin (OVA)-induced AR mouse model and perennial AR children, and the results indicated that it significantly ameliorates allergic airway inflammation. Here, we are the first to apply Lactobacillus plantarum GUANKE probiotic in AR.…”

Carbohydrate Hydrolytic Activity, Antibiotic Resistance and Stress Tolerance of <em>Lacticaseibacillus paracasei </em>BCRC-16100 and <em>Lacticaseibacillus paracasei</em> ZFM54 for Probiotics Using Genomic and Biochemical Approaches

“…The genus of the two strains is classified as probiotics but there is very little to know understanding on their health benefits, tolerance to stress and safety. Moreover, the species of the two strains are also recognized as probiotics only known for ameliorating allergic airway, antimicrobial activity, enhancement of intestinal microbiota and stress modulator, among others [53][54][55]. The intrinsic heterogeneity across strains of L. paracasei makes the species a strong group for the selection of probiotics isolates for health benefits and development of drugs or functional food for the abovementioned purpose [56].…”