Lactic acid production from sugarcane bagasse hydrolysates by Lactobacillus pentosus: Integrating xylose and glucose fermentation

Abstract

Lactic acid, traditionally obtained through fermentation process, presents numerous applications in different industrial segments, including production of biodegradable polylactic acid (PLA). Development of low cost substrate fermentations could improve economic viability of lactic acid production, through the use of agricultural residues as lignocellulosic biomass. Studies regarding the use of sugarcane bagasse hydrolysates for lactic acid production by Lactobacillus spp. are reported. First, five strains of Lactobacillus spp. were investigated for one that had the ability to consume xylose efficiently. Subsequently, biomass fractionation was performed by dilute acid and alkaline pretreatments, and the hemicellulose hydrolysate (HH) fermentability by the selected strain was carried out in bioreactor. Maximum lactic acid concentration and productivity achieved in HH batch were 42.5 g/L and 1.02 g/L h, respectively. Hydrolyses of partially delignified cellulignin (PDCL) by two different enzymatic cocktails were compared. Finally, fermentation of HH and PDCL hydrolysate together was carried out in bioreactor in a hybrid process: saccharification and co‐fermentation with an initial enzymatic hydrolysis. The high fermentability of these process herein developed was demonstrated by the total consumption of xylose and glucose by Lactobacillus pentosus, reaching at 65.0 g/L of lactic acid, 0.93 g/g of yield, and 1.01 g/L h of productivity. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 35: e2718, 2019

Complementary analyses of transcriptome and proteome revealed the formation mechanism of ethyl acetate, ethanol and organic acids in Kluyveromyces marxianus L1-1 in Chinese fermented acid rice soup

“…Interestingly, lactic acid has optical isomers: L-lactic acid and D-lactic acid, which can be produced by chemical synthesis (DL-lactic acid) or microbial fermentation (L-lactic acid, D-lactic acid, or DL-lactic acid). Compared to chemical synthesis processes, microbial fermentation processes present more advantages since they make use of renewable substrates from lactic acid bacteria [38]. Consistently, our study demonstrated that fermented rice-acid inoculated with K. marxianus L1-1 could produce more concentration of L-lactic acid in 3 day than 1 day, and the L-lactic acid has some advantages for healthy.…”

Production of Lactic Acid from Carob, Banana and Sugarcane Lignocellulose Biomass

“…of productivity). In another study, conducting simultaneous saccharification and co-fermentation (SSCF) with an initial pretreatment of the sugarcane bagasse using L. pentosus showed total consumption of both, xylose and glucose, producing 65.0 g LA•L −1 , and 0.93 g•g −1 of yield, where the productivity reached 1.01 g LA•L −1 •h −1 [3]. In another work, sugarcane bagasse hydrolysate obtained after pretreatment with dilute acid and alkaline was fermented using SHCF process for LA production by Lactobacillus spp, and resulted in a LA production and productivity of 42.5 g LA•L −1 and 1.02 g LA•L −1 •h, respectively.…”

“…The focus nowadays is on the use of different types of treatments used for the transformation of lignocellulosic biomass into suitable substrates for industrial production. Therefore, several methods of fermentations and genetic modifications are underway in order to increase the production and the scale up of LA process [3,12,13]. The biodegradability and biocompatibility of PLA makes the polymer safe and ideal for its application in a wide range of industries, with increased environmental awareness worldwide promoting the development of such technologies for a sustainable approach [14].…”

“…Lignocellulosic residues are composed of cellulose, hemicellulose, and lignin. Pretreatments are required, followed by enzymatic hydrolysis processes, where the released sugars are utilized for the production of economical products including bioethanol, bacteriocins, lipoteichoic acid, probiotics, biogas and LA [ 1 , 2 , 3 , 4 ]. The bioconversion of lignocellulose to LA is an important alternative for its valorization to produce LA to be utilized in food, cosmetic and pharmaceutical industry [ 4 , 5 ].…”

Kinetic characteristics of long‐term repeated fed‐batch (LtRFb) l‐lactic acid fermentation by a Bacillus coagulans strain

“…As an alternative to chemical methods, l‐ lactic acid can be produced by microbial fermentation, which has various advantages, including the absence of racemates and environmental friendliness [7]. The selection of l ‐lactic acid producers is a basic and vital step for laboratory and industrial production.…”