Washed cells of Streptococcus sanguis were used tO' form artificial plaque on the surface of bovine enamel and incubated underneath buffer solutions, initial pH 6, for 36 h at 3 7°C. The decrease in the microhardness of the enamel surface under fermenting "plaque" could be prevented with fluoride. Enamel under a fermenting "plaque" tool up significantly more (/'<0.01) fluoride than enamel under a non-fermenting "plaque" (initial E" in buffer: 10 parts/10'). The artificial plaque did not accumulate fluoride-Within fermenting "plaques", the pH decreased significandy more without fluoride IP