Lactic acid fermentation for refining proteins from green crops and obtaining a high quality feed product for monogastric animals

Santamaría-Fernández, Maria; Molinuevo-Salces, Beatriz; Kiel, Pauli; Steenfeldt, Sanna; Uellendahl, Hinrich; Lübeck, Mette

doi:10.1016/j.jclepro.2017.06.115

Cited by 55 publications

(54 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The reason for the low yield is, first, due to a high unknown loss of protein concentrate related to the two forced process stoppages caused by accumulation of product in the drying chamber. Second, the concentration of both DM and CP in the brown juice of 6% of juice and 19% of DM, respectively, was more than double the concentrations reported in previous studies . Thus, significant product potentials could have been obtained in the separation.…”

Section: Resultsmentioning

confidence: 67%

“…total sum of measured amino acids) content represented 29% DM. Previously, protein concentrates with slightly higher TP contents were produced from grass clover (35% DM) or red clover (40% DM) in laboratory setups . Moreover, protein concentrates produced by acid precipitation with sulfuric acid (pH = 4.0) at lab scale from the whole white clover plant presented CP content of 45% DM and TP contents of 39% DM, while higher CP content of 53% DM and higher TP content of 44% DM were achieved when the protein concentrate was produced only from white clover leaves .…”

Section: Resultsmentioning

confidence: 99%

“…34 The protein concentrate presented a low pH (4.2) and had some lactic acid (6.1% DM), glucose (1.4% DM), and a considerable amount of inorganics (10.6% DM). The concentration of each amino acid was slightly lower compared with the protein concentrates produced from clover grass and red clover in laboratory setups, 14 as shown in Table 4. Differences in the grass-to-clover ratio could be the reason for the variances between protein concentrates in terms of concentration of amino acids.…”

Section: Composition and Amino Acid Profile Of The Organic Protein Comentioning

confidence: 92%

“…Second, the concentration of both DM and CP in the brown juice of 6% of juice and 19% of DM, respectively, was more than double the concentrations reported in previous studies. 14,23 Thus, significant product potentials could have been obtained in the separation. It was, however, not the aim of the case production to optimize yields and process efficiencies.…”

Section: Operation Time (Hours)mentioning

confidence: 99%

“…13 L. salivarius strains were proven to be the fastest lactic acid bacteria during the fermentation of plant juice sugars into lactic acid, which resulted in a pH decrease to below 4.0 in the fermented juice. 13 Accordingly, lactic acid fermentation of the plant green juice with a fast-growing lactic acid bacteria might be a relevant method for the precipitation of proteins and production of protein concentrates, as indicated by Santamaria-Fernandez et al 14 Compared to heat coagulation, lactic acid fermentation requires less energy, which can improve the sustainability of the whole process. The acid precipitation of proteins by means of lactic acid fermentation also does not involve any chemicals, which allows the protein concentrate to be used in organic farming.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Demonstration‐scale protein recovery by lactic acid fermentation from grass clover – a single case of the production of protein concentrate and press cake silage for animal feeding trials

Santamaría-Fernández

Ambye‐Jensen

Damborg

et al. 2018

Biofuels Bioprod Bioref

View full text Add to dashboard Cite

There is an increasing interest in green biorefineries for the utilization of green biomass to provide a variety of valuable products. Proteins can be extracted from green biomasses such as alfalfa, clover, or grass and separated into protein‐rich concentrates suitable for feeding to monogastric animals. Press cake suitable for feeding ruminant animals is also produced. Full implementation of such a green biorefinery requires knowledge and experience of production at demonstration scale. Moreover, product evaluation in animal‐feeding trials requires a large amount of product. There has been collaboration in Denmark between several partners from industry and academia to assess the practicality and scalability of a green biorefining plant producing protein concentrate and press cake silage from organic grass clover at the scale of ca. 10 tons per hour fresh biomass input. Lactic acid fermentation of the pressed green juice was successfully performed to precipitate proteins and stabilize the juice. Despite minor difficulties, the demonstration‐scale biorefining plant was operative for four days, processed 444 tons of grass clover, and resulted in the overall production of 1 ton of dried protein concentrate, 7 tons of protein paste, and 223 tons of press cake silage. The protein concentrate was rich in essential amino acids and was tested in feeding trials with poultry; the press cake silage, rich in processed fibers and fiber‐associated proteins, was tested in feeding trials with dairy cows. © 2018 Society of Chemical Industry and John Wiley & Sons, Ltd

show abstract

Section: Resultsmentioning

confidence: 67%

Section: Resultsmentioning

confidence: 99%

Section: Composition and Amino Acid Profile Of The Organic Protein Comentioning

confidence: 92%

Section: Operation Time (Hours)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Demonstration‐scale protein recovery by lactic acid fermentation from grass clover – a single case of the production of protein concentrate and press cake silage for animal feeding trials

Santamaría-Fernández

Ambye‐Jensen

Damborg

et al. 2018

Biofuels Bioprod Bioref

View full text Add to dashboard Cite

show abstract

Annual protein yield and extractable protein potentials in three legumes and two grasses

Thers

Eriksen

2021

J Sci Food Agric

View full text Add to dashboard Cite

BACKGROUND New protein sources with low environmental and climatic impact are needed. Perennial crops show advantages as compared to annual crops and the upcoming biorefinery technology can extract proteins from the perennial biomass for protein concentrate production. The search for best‐suited biomass crops needs to include harvest during the full growing season to support economic viability of biorefinery plants. Here we examined two grasses under increasing N fertilizer regime (175, 350 and 525 kg N ha−1) and three legumes, subject to a four‐cut strategy. The well‐defined Cornell Net Carbohydrate and Protein System (CNCPS) was used to estimate potential extractable protein. A key, previously presented in the literature, was applied in order to translate the CNCPS results into potential extracted protein concentrate. RESULTS Crude protein (CP) yield per hectare was highest in red clover in 2015 (2907 kg CP ha−1) and the fertilized (525 kg N ha−1) tall fescue in 2016 (2435 kg CP ha−1). When translating the numbers into potential extraction of protein concentrate, the red clover had the highest protein concentrate yield per hectare in 2015 (835 kg CP ha−1) and lucerne in 2016 (803 kg CP ha−1). CONCLUSION The results revealed that the entire season needs attention for optimization and not only the first cut, since both CP yields and quality peaks in different cuts across the five species and 2 years. Further knowledge of CP yield responses to field management and species mixtures are needed in order to advise farmers on the optimal crop for biorefining. © 2021 Society of Chemical Industry.

show abstract

The composition and nutritional quality of biorefined lucerne protein depend on precipitation method

Stødkilde,

Ingerslev,

Ambye‐Jensen

et al. 2024

J Sci Food Agric

View full text Add to dashboard Cite

BACKGROUNDLucerne protein extract is a novel high‐quality protein source with excellent amino acid (AA) composition of interest for human consumption. In this study, protein from screw‐pressed lucerne juice was extracted by different precipitation methods to evaluate the effect on the chemical composition and nutritional quality of the extracted protein. Methods based on heat, acidification or fermentation were used for protein precipitation, and the nutritional value of protein was evaluated in a rat digestibility trial.RESULTSHeat precipitation at 85 °C produced a protein product with a crude protein (CP) content of 589 g kg−1 dry matter (DM), a balanced AA composition and a high standardized nitrogen (N) digestibility (82.8%). Precipitation by acidification, at a lower temperature (60 °C) or by fermentation, resulted in lower CP content (425–488 g kg−1 DM). Nitrogen digestibility for the pH‐adjusted precipitate was equal to the 85 °C heat‐precipitated protein, while the fermented and 60 °C precipitated proteins showed lower N digestibility (76.5% and 78.6%, respectively). By applying a two‐step heat precipitation method (60 °C followed by 80 °C), a protein content of 712 g kg−1 DM and an N digestibility of 93.6% was reached, which are comparable to high‐quality animal‐based protein sources such as milk, whey, casein, and eggs, covering the AA requirements for children >6 months.CONCLUSIONHigh‐quality protein can be extracted from lucerne, but the future focus should be on increased yield as the current low yields of the refined product will challenge the environmental and economic sustainability of production. © 2023 Society of Chemical Industry.

show abstract

Lactic acid fermentation for refining proteins from green crops and obtaining a high quality feed product for monogastric animals

Cited by 55 publications

References 30 publications

Demonstration‐scale protein recovery by lactic acid fermentation from grass clover – a single case of the production of protein concentrate and press cake silage for animal feeding trials

Demonstration‐scale protein recovery by lactic acid fermentation from grass clover – a single case of the production of protein concentrate and press cake silage for animal feeding trials

Annual protein yield and extractable protein potentials in three legumes and two grasses

The composition and nutritional quality of biorefined lucerne protein depend on precipitation method

Contact Info

Product

Resources

About