Lactic Acid Bacteria from Gamecock and Goat Originating from Phitsanulok, Thailand: Isolation, Identification, Technological Properties and Probiotic Potential

Abstract

From independent swab samples of the cloaca of indigenous gamecocks (CIG), anus of healthy baby goats (AHG), and vagina of goats (VG) originating from Phitsanulok, Thailand, a total of 263 isolates of lactic acid bacteria (LAB) were collected. Only three isolates, designated C707, G502, and V202, isolated from CIG, AHG, and VG, respectively, exhibited an excellent inhibitory zone diameter against foodborne pathogenic bacteria when evaluated by agar spot test. Isolates C707 and G502 were identified as Enterococcus faecium, whereas V202 was identified as Pediococcus acidilactici, based on 16S rRNA sequence analysis. When foodborne pathogenic bacteria were co-cultured with chosen LAB in mixed BHI-MRS broth at 39°C, their growth was suppressed. These LAB were found to be capable of surviving in simulated stomach conditions. Only the isolate G502 was able to survive in the conditions of simulated intestinal juice. This research suggests that selected LAB could be used as a food/feed supplement to reduce foodborne pathogenic bacteria and improve the safety of animal-based food or feed.

Selection and Characterization of Bacteriocinogenic Lactic Acid Bacteria from the Intestine of Gilthead Seabream (Sparus aurata) and Whiting Fish (Merlangius merlangus): Promising Strains for Aquaculture Probiotic and Food Bio-Preservation

“…monocytogenes , methicillin resistant S. aureus (MRSA), P. larvae , Vibrio alginolyticus , E. faecalis , B. cereus , and B. subtilis , were inhibited by 39, 37, 37, 16, 10, and 8 isolates, respectively. It is well known that LAB exhibit antibacterial activities against Gram-positive bacteria such as L. monocytogenes , S. aureus , E. faecalis , B. cereus , and B. subtilis [ 24 , 26 , 62 ]. Food was identified as the first source of infection of L. monocytogenes in humans, and today it is a public health concern related to septicemia, meningitis, gastroenteritis, pneumonia, and abortion [ 63 ].…”

“…Similarly, all isolates were able to grow in 1% bile salts. These findings are not surprising, indeed several studies, with few exceptions, reported that LAB can survive under gastric conditions (low pH) and resist bile salts; moreover, these traits are particularly observed in LAB species of human and animal microbiota [27,33,62]. Another important criterion to be considered when selecting potential probiotic candidates is the strain surface hydrophobicity; indeed, high hydrophobicity of LAB strains correlate with their attachment to intestinal mucosal and epithelial cells [8].…”