Lactic Acid Bacteria and Gut Health

Li, Haitao; Fang, Zhifeng

doi:10.1007/978-981-13-7832-4_8

Cited by 2 publications

(2 citation statements)

References 150 publications

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“…QS is a unique self-regulatory mechanism in which cells communicate through the release of autosensing molecules. This process induces the expression of relevant metabolic genes, regulating behaviors such as biofilm formation, bacteriocin synthesis, and bacterial autolysis ( Li et al, 2021 ). Bacterial density and autoinduction molecules are interconnected.…”

Section: Resultsmentioning

confidence: 99%

Transcriptomics of Lactobacillus paracasei: metabolism patterns and cellular responses under high-density culture conditions

Li,

Zhang,

Meng

et al. 2023

Front. Bioeng. Biotechnol.

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Lactobacillus paracasei has significant potential for development and application in the environmental field, particularly in addressing malodor pollution. This study aims to investigate the cellular response of L. paracasei B1 under high-density culture conditions. The selected strain has previously shown effective deodorizing and bacteriostatic abilities. Transcriptomics techniques are employed to dissect the nutrient metabolism pattern of L. paracasei B1 and its response mechanism under environmental stress. The study characterizes the functions of key differentially expressed genes during growth before and after optimizing the culture conditions. The optimization of fermentation culture conditions provides a suitable growth environment for L. paracasei B1, inducing an enhancement of its phosphotransferase system for sugar source uptake and maintaining high levels of glycolysis and pyruvate metabolism. Consequently, the strain is able to grow and multiply rapidly. Under acid stress conditions, glycolysis and pyruvate metabolism are inhibited, and L. paracasei B1 generates additional energy through aerobic respiration to meet the energy demand. The two-component system and quorum sensing play roles in the response and regulation of L. paracasei B1 to adverse environments. The strain mitigates oxygen stress damage through glutathione metabolism, cysteine and methionine metabolism, base excision repair, and purine and pyrimidine metabolism. Additionally, the strain enhances lysine synthesis, the alanine, aspartate, and glutamate metabolic pathways, and relies on the ABC transport system to accumulate amino acid-compatible solutes to counteract acid stress and osmotic stress during pH regulation. These findings establish a theoretical basis for the further development and application of L. paracasei B1 for its productive properties.

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Section: Resultsmentioning

confidence: 99%

Transcriptomics of Lactobacillus paracasei: metabolism patterns and cellular responses under high-density culture conditions

Li,

Zhang,

Meng

et al. 2023

Front. Bioeng. Biotechnol.

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“…Research on high-density fermentation in different strains indicates that the most important factors that affect the high-density growth of yeast cells in batch fermentation are the ingredients in the medium and the conditions of fermentation [20][21][22][23][24]. Research has shown that glucose, sucrose, yeast extract, peptone, KH 2 PO 4 , MgSO 4 , and CuSO 4 are the main nutrients that promote yeast cell growth; at the same time, the pH of the medium, inoculum size, rotation speed, liquid volume, fermentation temperature and fermentation time are the key fermentation conditions affecting yeast cell growth [23].…”

Section: Introductionmentioning

confidence: 99%

Optimization of High-Density Fermentation Conditions for Saccharomycopsis fibuligera Y1402 through Response Surface Analysis

Yuan,

Sun,

Wang

et al. 2024

Foods

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Saccharomycopsis fibuligera, which produces enzymes like amylase and protease as well as flavor substances like β-phenyl ethanol and phenyl acetate, plays a crucial role in traditional fermented foods. However, this strain still lacks a high-density fermentation culture, which has had an impact on the strain’s industrial application process. Therefore, this study investigated the optimization of medium ingredients and fermentation conditions for high-density fermentation of S. fibuligera Y1402 through single-factor design, Plackett–Burman design, steepest ascent test, and response surface analysis. The study found that glucose at 360.61 g/L, peptone at 50 g/L, yeast extract at 14.65 g/L, KH2PO4 at 5.49 g/L, MgSO4 at 0.40 g/L, and CuSO4 at 0.01 g/L were the best medium ingredients for S. fibuligera Y1402. Under these conditions, after three days of fermentation, the total colony count reached 1.79 × 108 CFU/mL. The optimal fermentation conditions were determined to be an initial pH of 6.0, an inoculum size of 1.10%, a liquid volume of 12.5 mL/250 mL, a rotation speed of 120 r/min, a fermentation temperature of 21 °C and a fermentation time of 53.50 h. When fermentation was conducted using the optimized medium and conditions, the total colony count achieved a remarkable value of 5.50 × 109 CFU/mL, exhibiting a substantial increase of nearly 31 times the original value in the optimal culture medium. This significant advancement offers valuable insights and a reference for the industrial-scale production of S. fibuligera.

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Lactic Acid Bacteria and Gut Health

Cited by 2 publications

References 150 publications

Transcriptomics of Lactobacillus paracasei: metabolism patterns and cellular responses under high-density culture conditions

Transcriptomics of Lactobacillus paracasei: metabolism patterns and cellular responses under high-density culture conditions

Optimization of High-Density Fermentation Conditions for Saccharomycopsis fibuligera Y1402 through Response Surface Analysis

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